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Author = Meade, Kieran;
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Displaying Results 1 - 2 of 2 on page 1 of 1
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?-Defensins: Farming the microbiome for homeostasis and health
(2019)
O'Farrelly, Cliona; Meade, Kieran
?-Defensins: Farming the microbiome for homeostasis and health
(2019)
O'Farrelly, Cliona; Meade, Kieran
Abstract:
Diverse commensal populations are now regarded as key to physiological homeostasis and protection against disease. Although bacteria are the most abundant component of microbiomes, and the most intensively studied, the microbiome also consists of viral, fungal, archael, and protozoan communities, about which comparatively little is known. Host-defense peptides (HDPs), originally described as antimicrobial, now have renewed significance as curators of the pervasive microbial loads required to maintain homeostasis and manage microbiome diversity. Harnessing HDP biology to transition away from non-selective, antibiotic-mediated treatments for clearance of microbes is a new paradigm, particularly in veterinary medicine. One family of evolutionarily conserved HDPs, ?-defensins which are produced in diverse combinations by epithelial and immune cell populations, are multifunctional cationic peptides which manage the cross-talk between host and microbes and maintain a healthy yet dynamic e...
http://hdl.handle.net/2262/89582
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Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle
(2018)
O'Farrelly, Cliona; Adnane, Mounir; Kelly, Paul; Chapwanya, Aspinas; Meade, Kieran
Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle
(2018)
O'Farrelly, Cliona; Adnane, Mounir; Kelly, Paul; Chapwanya, Aspinas; Meade, Kieran
Abstract:
Background: In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved methodfor processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and?1-acid glycoprotein (AGP) were measured by ELISA. Results: We found that use of reducing agents to liquefy CVM affects protein yield and the accuracy of biomarker detection. Our...
http://hdl.handle.net/2262/89581
Displaying Results 1 - 2 of 2 on page 1 of 1
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