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Author = SCHIPANI, ROSSANA;
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Displaying Results 1 - 2 of 2 on page 1 of 1
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3D bioprinting of cartilage-mimetic implants for biological joint resurfacing
(2020)
SCHIPANI, ROSSANA
3D bioprinting of cartilage-mimetic implants for biological joint resurfacing
(2020)
SCHIPANI, ROSSANA
Abstract:
A major challenge in the field of tissue engineering and regenerative medicine is the development of effective therapies for treating large cartilage or osteochondral defects and ultimately regenerating whole osteoarthritic joints. The objective of this thesis was to 3D bioprint cell-laden biomaterials with biomimetic mechanical properties as implants for regenerating large osteochondral defects. To this end, a finite element modelling (FEM) strategy was first developed to design the 3D printed polycaprolactone (PCL) networks with user-defined mechanical properties. These PCL networks were then combined with an alginate-gelatin methacryloyl (gelMA) interpenetrating network (IPN) hydrogel to develop 3D bioprinted constructs that were both mechanically functional and supportive of mesenchymal stromal cells (MSCs) chondrogenesis. When the IPN hydrogels were reinforced with a PCL network characterized by relatively high tension-compression nonlinearity, the resulting composites possesse...
http://hdl.handle.net/2262/93022
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Fiber Reinforced Cartilage ECM Functionalized Bioinks for Functional Cartilage Tissue Engineering
(2019)
Moebius, Matthias; Kelly, Daniel; Rathan, Swetha; Dejob, L?a; Schipani, Rossana; Haffne...
Fiber Reinforced Cartilage ECM Functionalized Bioinks for Functional Cartilage Tissue Engineering
(2019)
Moebius, Matthias; Kelly, Daniel; Rathan, Swetha; Dejob, L?a; Schipani, Rossana; Haffner, Benjamin
Abstract:
Focal articular cartilage (AC) defects, if left untreated, can lead to debilitating diseases such as osteoarthritis. While several tissue engineering strategies have been developed to promote cartilage regeneration, it is still challenging to generate functional AC capable of sustaining high load-bearing environments. We developed a new class of cartilage extracellular matrix (cECM)-functionalized alginate bioink for the bioprinting of cartilaginous tissues. The bioinks were 3D-printable, supported mesenchymal stem cell (MSC) viability post-printing and robust chondrogenesis in vitro, with the highest levels of COLLII and ACAN expression observed in bioinks containing the highest concentration of cECM. Enhanced chondrogenesis in cECM-functionalized bioinks was also associated with progression along an endochondral-like pathway, as evident by increases in RUNX2 expression and calcium deposition in vitro. The bioinks loaded with MSCs and TGF-?3 were also found capable of supporting ro...
http://hdl.handle.net/2262/91275
Displaying Results 1 - 2 of 2 on page 1 of 1
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Doctoral thesis (1)
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2020 (1)
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