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Author = STANTON, CATHERINE;
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Displaying Results 1 - 25 of 173 on page 1 of 7
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16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform
(2016)
Fouhy, Fiona; Clooney, Adam G; STANTON, CATHERINE; Claesson, Marcus J.; Cotter, Paul D.
16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform
(2016)
Fouhy, Fiona; Clooney, Adam G; STANTON, CATHERINE; Claesson, Marcus J.; Cotter, Paul D.
Abstract:
Background Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer sequences and sequencing platform employed, can affect the accuracy of the results achieved. The aim of this study was to determine the impact of these three factors on 16S rRNA gene sequencing results, using mock communities and mock community DNA. Results The use of different primer sequences (V4-V5, V1-V2 and V1-V2 degenerate primers) resulted in differences in the genera and species detected. The V4-V5 primers gave the most comparable results across platforms. The three Ion PGM primer sets detected more of the 20 mock community species than the equivalent MiSeq primer sets. Data generated from DNA extracted using ...
http://hdl.handle.net/11019/1018
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16S rRNA gene sequencing of mock microbial populations-impact of DNA extraction method, primer choice and sequencing platform
(2016)
Fouhy, Fiona; Clooney, Adam G.; Stanton, Catherine; Claesson, Marcus J.; Cotter, Paul D.
16S rRNA gene sequencing of mock microbial populations-impact of DNA extraction method, primer choice and sequencing platform
(2016)
Fouhy, Fiona; Clooney, Adam G.; Stanton, Catherine; Claesson, Marcus J.; Cotter, Paul D.
Abstract:
Background: Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer sequences and sequencing platform employed, can affect the accuracy of the results achieved. The aim of this study was to determine the impact of these three factors on 16S rRNA gene sequencing results, using mock communities and mock community DNA. Results: The use of different primer sequences (V4-V5, V1-V2 and V1-V2 degenerate primers) resulted in differences in the genera and species detected. The V4-V5 primers gave the most comparable results across platforms. The three Ion PGM primer sets detected more of the 20 mock community species than the equivalent MiSeq primer sets. Data generated from DNA extracted using the 2 extraction methods were very similar. Conclusions: Microbiota composit...
http://hdl.handle.net/10468/4126
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48 Altered gut microbiota in stable patients with cystic fibrosis (CF) compared to controls and its relationship with intravenous (IV) antibiotic usage and lung function
(2020)
Burke, D.G.; Fouhy, Fiona; Rea, Mary; Harrison, M.J.; STANTON, CATHERINE; O’Sullivan, O...
48 Altered gut microbiota in stable patients with cystic fibrosis (CF) compared to controls and its relationship with intravenous (IV) antibiotic usage and lung function
(2020)
Burke, D.G.; Fouhy, Fiona; Rea, Mary; Harrison, M.J.; STANTON, CATHERINE; O’Sullivan, Orla; Murphy, D. M.; O'Callaghan, G. P.; Eustace, J. A.; Shanahan, F.; Fleming, C.; McCarthy, M.; Shortt, C.; Ross, R Paul; Plant, B. J.
Abstract:
Objective CF is associated with altered digestive function and thus nutrient availability for gut microbes in addition to altered gut microbiota, compared with healthy controls. Equally intensive antibiotic and nutritional therapy may further compound this. We present results from the largest CF gut microbiota study to date.
http://hdl.handle.net/11019/1898
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A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and ß-lactam resistance genes in the gut microbiota
(2014)
Fouhy, Fiona; Ross, R P; Fitzgerald, Gerald F; Stanton, Catherine; Cotter, Paul D
A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and ß-lactam resistance genes in the gut microbiota
(2014)
Fouhy, Fiona; Ross, R P; Fitzgerald, Gerald F; Stanton, Catherine; Cotter, Paul D
Abstract:
Abstract Background The potential for the human gut microbiota to serve as a reservoir for antibiotic resistance genes has been the subject of recent discussion. However, this has yet to be investigated using a rapid PCR-based approach. In light of this, here we aim to determine if degenerate PCR primers can detect aminoglycoside and β-lactam resistance genes in the gut microbiota of healthy adults, without the need for an initial culture-based screen for resistant isolates. In doing so, we would determine if the gut microbiota of healthy adults, lacking recent antibiotic exposure, is a reservoir for resistance genes. Results The strategy employed resulted in the identification of numerous aminoglycoside (acetylation, adenylation and phosphorylation) and β-lactam (including bla OXA, bla TEM, bla SHV and bla CTX-M) resistance gene homologues. On the basis of homology, it would appear that ...
http://dx.doi.org/10.1186/1471-2180-14-25
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A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and ß-lactam resistance genes in the gut microbiota
(2014)
Fouhy, Fiona; Ross, R Paul; Fitzgerald, Gerald F; STANTON, CATHERINE; Cotter, Paul D.
A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and ß-lactam resistance genes in the gut microbiota
(2014)
Fouhy, Fiona; Ross, R Paul; Fitzgerald, Gerald F; STANTON, CATHERINE; Cotter, Paul D.
Abstract:
Background: The potential for the human gut microbiota to serve as a reservoir for antibiotic resistance genes has been the subject of recent discussion. However, this has yet to be investigated using a rapid PCR-based approach. In light of this, here we aim to determine if degenerate PCR primers can detect aminoglycoside and β-lactam resistance genes in the gut microbiota of healthy adults, without the need for an initial culture-based screen for resistant isolates. In doing so, we would determine if the gut microbiota of healthy adults, lacking recent antibiotic exposure, is a reservoir for resistance genes. Results: The strategy employed resulted in the identification of numerous aminoglycoside (acetylation, adenylation and phosphorylation) and β-lactam (including bla OXA, bla TEM, bla SHV and bla CTX-M) resistance gene homologues. On the basis of homology, it would appear that these genes originated from different bacterial taxa, with members of the Enterobacteriaceae being a pa...
http://hdl.handle.net/11019/733
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A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota
(2014)
Fouhy, Fiona; Ross, R. Paul; Fitzgerald, Gerald F.; Stanton, Catherine; Cotter, Paul D.
A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota
(2014)
Fouhy, Fiona; Ross, R. Paul; Fitzgerald, Gerald F.; Stanton, Catherine; Cotter, Paul D.
Abstract:
Background: The potential for the human gut microbiota to serve as a reservoir for antibiotic resistance genes has been the subject of recent discussion. However, this has yet to be investigated using a rapid PCR-based approach. In light of this, here we aim to determine if degenerate PCR primers can detect aminoglycoside and β-lactam resistance genes in the gut microbiota of healthy adults, without the need for an initial culture-based screen for resistant isolates. In doing so, we would determine if the gut microbiota of healthy adults, lacking recent antibiotic exposure, is a reservoir for resistance genes. Results: The strategy employed resulted in the identification of numerous aminoglycoside (acetylation, adenylation and phosphorylation) and β-lactam (including bla OXA, bla TEM, bla SHV and bla CTX-M) resistance gene homologues. On the basis of homology, it would appear that these genes originated from different bacterial taxa, with members of the Enterobacteriaceae being a pa...
http://hdl.handle.net/10468/2253
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A pilot study demonstrating the altered gut microbiota functionality in stable adults with Cystic Fibrosis
(2020)
Fouhy, Fiona; Ronan, N. J.; O'Sullivan, Orla; McCarthy, Y.; Walsh, Aaron M.; Murph...
A pilot study demonstrating the altered gut microbiota functionality in stable adults with Cystic Fibrosis
(2020)
Fouhy, Fiona; Ronan, N. J.; O'Sullivan, Orla; McCarthy, Y.; Walsh, Aaron M.; Murphy, D.M.; Daly, M.; Flanagan, E. T.; Fleming, C.; McCarthy, M.; Shortt, C.; Eustace, J. A.; Shanahan, F.; Rea, Mary; Ross, R Paul; STANTON, CATHERINE; Plant, B. J.
Abstract:
Cystic Fibrosis (CF) and its treatment result in an altered gut microbiota composition compared to non-CF controls. However, the impact of this on gut microbiota functionality has not been extensively characterised. Our aim was to conduct a proof-of-principle study to investigate if measurable changes in gut microbiota functionality occur in adult CF patients compared to controls. Metagenomic DNA was extracted from faecal samples from six CF patients and six non-CF controls and shotgun metagenomic sequencing was performed on the MiSeq platform. Metabolomic analysis using gas chromatography-mass spectrometry was conducted on faecal water. The gut microbiota of the CF group was significantly different compared to the non-CF controls, with significantly increased Firmicutes and decreased Bacteroidetes. Functionality was altered, with higher pathway abundances and gene families involved in lipid (e.g. PWY 6284 unsaturated fatty acid biosynthesis (p = 0.016)) and xenobiotic metabolism (e...
http://hdl.handle.net/11019/1937
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Actinomyces produces defensin-like bacteriocins (Actifensins) with a highly degenerate structure and broad antimicrobial activity
(2020)
Sugrue, Ivan; O'Connor, Paula M.; Hill, Colin; Stanton, Catherine; Ross, R. Paul
Actinomyces produces defensin-like bacteriocins (Actifensins) with a highly degenerate structure and broad antimicrobial activity
(2020)
Sugrue, Ivan; O'Connor, Paula M.; Hill, Colin; Stanton, Catherine; Ross, R. Paul
Abstract:
We identified a strain of Actinomyces ruminicola which produces a potent bacteriocin with activity against a broad range of Gram-positive bacteria, many of which are pathogenic to animals and humans. The bacteriocin was purified and found to have a mass of 4,091 ± 1 Da with a sequence of GFGCNLITSNPYQCSNHCKSVGYRGGYCKLRTVCTCY containing three disulfide bridges. Surprisingly, near relatives of actifensin were found to be a series of related eukaryotic defensins displaying greater than 50% identity to the bacteriocin. A pangenomic screen further revealed that production of actifensin-related bacteriocins is a common trait within the genus, with 47 being encoded in 161 genomes. Furthermore, these bacteriocins displayed a remarkable level of diversity with a mean amino acid identity of only 52% between strains/species. This level of redundancy suggests that this new class of bacteriocins may provide a very broad structural basis on which to deliver and design new broad-spectrum antimicro...
http://hdl.handle.net/10468/9795
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Adding value to milk by increasing its protein and CLA contents
(2015)
Murphy, J.J.; STANTON, CATHERINE; O'Donovan, Michael; Kavanagh, S.; Maher, J.; Pat...
Adding value to milk by increasing its protein and CLA contents
(2015)
Murphy, J.J.; STANTON, CATHERINE; O'Donovan, Michael; Kavanagh, S.; Maher, J.; Patton, Joe; Mohammed, Riaz
Abstract:
End of project report
The mid-summer milk protein study was undertaken on 34 commercial dairy farms in 2005 to evaluate the influence of dietary and management variables on milk protein content in mid-season. Data on grass composition, genetic merit of the herds and milk protein content were collected and analysed by multiple regression. Both calving date and genetic merit for milk protein content were significantly associated with milk protein content and were used as adjustment factors when evaluating the association between measures of grass quality and milk protein content. Milk protein content was associated with grass OMD (P = 0.04) and NDF content (P = 0.02) but not with CP content (P = 0.80). It is concluded that herds calving earlier, with a greater genetic merit for milk protein content and consuming better quality pasture would have greater milk protein contents in mid-season.
http://hdl.handle.net/11019/830
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Adding value to milk by increasing its protein and CLA contents.
(2017)
Murphy, J.J.; STANTON, CATHERINE; O'Donovan, Michael; Kavanagh, S.; Maher, J.; Pat...
Adding value to milk by increasing its protein and CLA contents.
(2017)
Murphy, J.J.; STANTON, CATHERINE; O'Donovan, Michael; Kavanagh, S.; Maher, J.; Patton, Joe; Mohammed, Riaz
Abstract:
End of Project Report
Five experiments were undertaken in this project; one on mid-summer milk protein and four on milk CLA content. Thus the two main objectives of this project were to determine the factors associated with milk protein concentration in mid-summer and to investigate potential further strategies to increase the CLA content of pasture produced milk.
http://hdl.handle.net/11019/1186
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Application of Probiotic Bacteria to Functional Foods
(2017)
STANTON, CATHERINE; Ross, R Paul; Fitzgerald, Gerald F; Collins, K.; McBrearty, S.; Gar...
Application of Probiotic Bacteria to Functional Foods
(2017)
STANTON, CATHERINE; Ross, R Paul; Fitzgerald, Gerald F; Collins, K.; McBrearty, S.; Gardiner, Gillian E.; Desmond, C.; Kelly, J.; Bouchier, Paul J.; Lawless, Fergal; Auty, Mark; Corcoran, M.O.; Mullins, C.; Mullholand, E.; Eason, D.
Abstract:
End of Project Report
Probiotic cultures are described as live microbial feed supplements that improve intestinal microbial balance and are intended for maintenance of health or prevention, rather than the curing of disease. The demand for probiotic foods is increasing in Europe, Japan and the U.S. reflecting the heightened awareness among the public of the relationship between diet and health. Traditionally, the most popular food delivery systems for these cultures have been freshly fermented dairy foods, such as yogurts and fermented milks, as well as unfermented milks with cultures added. However, in the development of functional foods, the technological suitability of probiotic strains poses a serious challenge since their survival and viability may be adversely affected by processing conditions as well as by the product environment and storage conditions. This is a particular concern, given that high levels (at least 107 per gram or ml) of live micro-organisms are recommend...
http://hdl.handle.net/11019/1280
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Bacterial conjugated linoleic acid production and their applications
(2020)
Yang, Bo; Gao, He; STANTON, CATHERINE; Ross, R Paul; Zhang, Hao; Chen, Yong Q.; Chen, H...
Bacterial conjugated linoleic acid production and their applications
(2020)
Yang, Bo; Gao, He; STANTON, CATHERINE; Ross, R Paul; Zhang, Hao; Chen, Yong Q.; Chen, Haiqin; Chen, Wei
Abstract:
Conjugated linoleic acid (CLA) has been shown to exert various potential physiological properties including anti-carcinogenic, anti-obesity, anti-cardiovascular and anti-diabetic activities, and consequently has been considered as a promising food supplement. Bacterial biosynthesis of CLA is an attractive approach for commercial production due to its high isomer-selectivity and convenient purification process. Many bacterial species have been reported to convert free linoleic acid (LA) to CLA, hitherto only the precise CLA-producing mechanisms in Propionibacterium acnes and Lactobacillus plantarum have been illustrated completely, prompting the development of recombinant technology used in CLA production. The purpose of the article is to review the bacterial CLA producers as well as the recent progress on describing the mechanism of microbial CLA-production. Furthermore, the advances and potential in the heterologous expression of CLA genetic determinants will be presented.
http://hdl.handle.net/11019/2007
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Behavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the rat
(2016)
Hoban, Alan E.; Moloney, Rachel D.; Golubeva, Anna V.; McVey Neufeld, Karen A.; O'...
Behavioural and neurochemical consequences of chronic gut microbiota depletion during adulthood in the rat
(2016)
Hoban, Alan E.; Moloney, Rachel D.; Golubeva, Anna V.; McVey Neufeld, Karen A.; O'Sullivan, Orla; Patterson, Elaine; Stanton, Catherine; Dinan, Timothy G.; Clarke, Gerard; Cryan, John F.
Abstract:
Gut microbiota colonization is a key event for host physiology that occurs early in life. Disruption of this process leads to altered brain development which ultimately manifests as changes in brain function and behaviour in adulthood. Studies using germ-free mice highlight the extreme impact on brain health that results from life without commensal microbes, however the impact of microbiota disturbances occurring in adulthood is less studied. To this end, we depleted the gut microbiota of 10-week-old male Sprague Dawley rats via chronic antibiotic treatment. Following this marked, sustained depletion of the gut bacteria, we investigated behavioural and molecular hallmarks of gut-brain communication. Our results reveal that depletion of the gut microbiota during adulthood results in deficits in spatial memory as tested by Morris water maze, increased visceral sensitivity and a greater display of depressive-like behaviours in the forced swim test. In tandem with these clear behavioura...
http://hdl.handle.net/10468/3290
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Bifidobacterium and Lactobacillus composition at species level and gut microbiota diversity in infants before 6 weeks
(2019)
Yang, Bo; Chen, Yingqi; Stanton, Catherine; Ross, R. Paul; Lee, Yuan-Kun; Zhao, Jianxin...
Bifidobacterium and Lactobacillus composition at species level and gut microbiota diversity in infants before 6 weeks
(2019)
Yang, Bo; Chen, Yingqi; Stanton, Catherine; Ross, R. Paul; Lee, Yuan-Kun; Zhao, Jianxin; Zhang, Hao; Chen, Wei
Abstract:
Our objective was to investigate the effects of different delivery and feeding modes on the gut microbiota composition of early infants with special emphasis on Bifidobacterium and Lactobacillus profiles at species level. 16S rRNA V3-V4 regions, bifidobacterial, and lactobacilli groEL genes from infant feces were sequenced by Illumina MiSeq. Gut microbiota abundance was significantly different, where standard vaginally delivered (SVD) and breast-fed (BF) groups were higher in comparison with caesarean section (CS), milk-powder-fed (MPF), and mixed-fed (MF) groups. The genus unclassified Enterobacteriaceae was dominant, followed by Bifidobacterium, which was highly abundant in SVD and BF groups. The dominant Bifidobacterium species in all groups were B. longum subsp. longum, B. longum subsp. infantis and B. animalis subsp. lactis. B. dentium and the diversity of Bifidobacterium in SVD and BF groups were significantly higher. For Lactobacillus profiles, L. rhamnosus and L. gasseri wer...
http://hdl.handle.net/10468/8591
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Bifidobacterium breve with a-linolenic acid alters the composition, distribution and transcription factor activity associated with metabolism and absorption of fat
(2017)
Patterson, Elaine; Wall, Rebecca; Lisai, Sara; Ross, R. Paul; Dinan, Timothy G.; Cryan,...
Bifidobacterium breve with a-linolenic acid alters the composition, distribution and transcription factor activity associated with metabolism and absorption of fat
(2017)
Patterson, Elaine; Wall, Rebecca; Lisai, Sara; Ross, R. Paul; Dinan, Timothy G.; Cryan, John F.; Fitzgerald, Gerald F.; Banni, Sebastiano; Quigley, Eamonn M.; Shanahan, Fergus; Stanton, Catherine
Abstract:
This study focused on the mechanisms that fatty acid conjugating strains - Bifidobacterium breve NCIMB 702258 and Bifidobacterium breve DPC 6330 - influence lipid metabolism when ingested with α-linolenic acid (ALA) enriched diet. Four groups of BALB/c mice received ALA enriched diet (3% (w/w)) either alone or in combination with B. breve NCIMB 702258 or B. breve DPC 6330 (109 CFU/day) or unsupplemented control diet for six weeks. The overall n-3 PUFA score was increased in all groups receiving the ALA enriched diet. Hepatic peroxisomal beta oxidation increased following supplementation of the ALA enriched diet with B. breve (P < 0.05) and so the ability of the strains to produce c9t11 conjugated linoleic acid (CLA) was identified in adipose tissue. Furthermore, a strain specific effect of B. breve NCIMB 702258 was found on the endocannabinoid system (ECS). Liver triglycerides (TAG) were reduced following ALA supplementation, compared with unsupplemented controls (P < 0.01) wh...
http://hdl.handle.net/10468/3785
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Bifidobacterium breve with α-Linolenic Acid and Linoleic Acid Alters Fatty Acid Metabolism in the Maternal Separation Model of Irritable Bowel Syndrome
(2015)
Barrett, Eoin; Fitzgerald, Patrick; Dinan, Timothy G.; Cryan, John F.; Ross, R Paul; Qu...
Bifidobacterium breve with α-Linolenic Acid and Linoleic Acid Alters Fatty Acid Metabolism in the Maternal Separation Model of Irritable Bowel Syndrome
(2015)
Barrett, Eoin; Fitzgerald, Patrick; Dinan, Timothy G.; Cryan, John F.; Ross, R Paul; Quigley, Eamonn M.; Shanahan, Fergus; Kiely, Barry; Fitzgerald, Gerald F; O'Toole, Paul W.; STANTON, CATHERINE
Abstract:
The aim of this study was to compare the impact of dietary supplementation with a Bifidobacterium breve strain together with linoleic acid & α-linolenic acid, for 7 weeks, on colonic sensitivity and fatty acid metabolism in rats. Maternally separated and non-maternally separated Sprague Dawley rats (n = 15) were orally gavaged with either B. breve DPC6330 (109 microorganisms/day) alone or in combination with 0.5% (w/w) linoleic acid & 0.5% (w/w) α-linolenic acid, daily for 7 weeks and compared with trehalose and bovine serum albumin. Tissue fatty acid composition was assessed by gas-liquid chromatography and visceral hypersensitivity was assessed by colorectal distension. Significant differences in the fatty acid profiles of the non-separated controls and maternally separated controls were observed for α-linolenic acid and arachidonic acid in the liver, oleic acid and eicosenoic acid (c11) in adipose tissue, and for palmitoleic acid and docosahexaenoic acid in serum (p<0....
http://hdl.handle.net/11019/770
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Bile acids at the cross-roads of gut microbiome–host cardiometabolic interactions
(2017)
Ryan, Paul M.; Stanton, Catherine; Caplice, Noel M.
Bile acids at the cross-roads of gut microbiome–host cardiometabolic interactions
(2017)
Ryan, Paul M.; Stanton, Catherine; Caplice, Noel M.
Abstract:
While basic and clinical research over the last several decades has recognized a number of modifiable risk factors associated with cardiometabolic disease progression, additional and alternative biological perspectives may offer novel targets for prevention and treatment of this disease set. There is mounting preclinical and emerging clinical evidence indicating that the mass of metabolically diverse microorganisms which inhabit the human gastrointestinal tract may be implicated in initiation and modulation of cardiovascular and metabolic disease outcomes. The following review will discuss this gut microbiome–host metabolism axis and address newly proposed bile-mediated signaling pathways through which dysregulation of this homeostatic axis may influence host cardiovascular risk. With a central focus on the major nuclear and membrane-bound bile acid receptor ligands, we aim to review the putative impact of microbial bile acid modification on several major phenotypes of metabolic syn...
http://hdl.handle.net/10468/5402
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Bile acids at the cross-roads of gut microbiome–host cardiometabolic interactions
(2018)
Ryan, Paul M; STANTON, CATHERINE; Caplice, Noel M
Bile acids at the cross-roads of gut microbiome–host cardiometabolic interactions
(2018)
Ryan, Paul M; STANTON, CATHERINE; Caplice, Noel M
Abstract:
This work is supported in part by the APC Microbiome Institute (under Science Foundation Ireland [SFI] Grant Number: SFI/12/RC/2273) and by Enterprise Ireland Commercialization Fund (Contract Reference: CF/2013/3030A/B).
While basic and clinical research over the last several decades has recognized a number of modifiable risk factors associated with cardiometabolic disease progression, additional and alternative biological perspectives may offer novel targets for prevention and treatment of this disease set. There is mounting preclinical and emerging clinical evidence indicating that the mass of metabolically diverse microorganisms which inhabit the human gastrointestinal tract may be implicated in initiation and modulation of cardiovascular and metabolic disease outcomes. The following review will discuss this gut microbiome–host metabolism axis and address newly proposed bile-mediated signaling pathways through which dysregulation of this homeostatic axis may influence host ca...
http://hdl.handle.net/11019/1529
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Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health
(2020)
Lyons, Katríona E.; Ryan, C. Anthony; Dempsey, Eugene M.; Ross, R Paul; STANTON, CATHERINE
Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health
(2020)
Lyons, Katríona E.; Ryan, C. Anthony; Dempsey, Eugene M.; Ross, R Paul; STANTON, CATHERINE
Abstract:
Human breast milk is considered the optimum feeding regime for newborn infants due to its ability to provide complete nutrition and many bioactive health factors. Breast feeding is associated with improved infant health and immune development, less incidences of gastrointestinal disease and lower mortality rates than formula fed infants. As well as providing fundamental nutrients to the growing infant, breast milk is a source of commensal bacteria which further enhance infant health by preventing pathogen adhesion and promoting gut colonisation of beneficial microbes. While breast milk was initially considered a sterile fluid and microbes isolated were considered contaminants, it is now widely accepted that breast milk is home to its own unique microbiome. The origins of bacteria in breast milk have been subject to much debate, however, the possibility of an entero-mammary pathway allowing for transfer of microbes from maternal gut to the mammary gland is one potential pathway. Huma...
http://hdl.handle.net/11019/2182
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c9, t11, c15-CLNA and c9, t11, t15-CLNA from Lactobacillus plantarum ZS2058 ameliorate DSS-induced colitis in mice
(2020)
Ren, Qing; Yang, Bo; Zhang, Hao; Ross, R. Paul; Stanton, Catherine; Chen, Haiqin; Chen,...
c9, t11, c15-CLNA and c9, t11, t15-CLNA from Lactobacillus plantarum ZS2058 ameliorate DSS-induced colitis in mice
(2020)
Ren, Qing; Yang, Bo; Zhang, Hao; Ross, R. Paul; Stanton, Catherine; Chen, Haiqin; Chen, Wei
Abstract:
The probiotic bacterial strain Lactobacillus plantarum ZS2058 has been proven to alleviate colitis due to its ability to synthesize conjugated fatty acids (CFAs). To investigate the specific functions of CFAs produced by this probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated α-linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and c9, t11, t15-CLNA (CLNA2). The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored. CLNA significantly inhibited weight loss, the disease activity index, colon shortening. Additionally, CLNA improved histological damage, protected colonic mucous layer integrity and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, and claudin-3). CLNA significantly attenuated the expression of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-i...
http://hdl.handle.net/10468/9746
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Characterization of protein hydrolysates from blue whiting (Micromesistius poutassou) and their application in beverage fortification
(2017)
Egerton, Sian; Culloty, Sarah C.; Whooley, Jason; Stanton, Catherine; Ross, R. Paul
Characterization of protein hydrolysates from blue whiting (Micromesistius poutassou) and their application in beverage fortification
(2017)
Egerton, Sian; Culloty, Sarah C.; Whooley, Jason; Stanton, Catherine; Ross, R. Paul
Abstract:
Enzymatic hydrolysis of fish proteins has been employed as a principle method for converting under-utilised fish into valuable products for the pharmaceutical and health food industries. In this study, six commercial enzymes were tested for their ability to make fish protein hydrolysate powders from whole blue whiting. The chemical and functional properties of these powders were compared. The powders all had high solubility (>80%) across a wide pH range in water and their solubility improved further within a vitamin-tea beverage matrix (>85%). Varying degrees of anti-oxidant activities were recorded for the powders using three model systems (DPPH, ferrous chelating and reducing power). This study demonstrates that commercial enzymes are useful for the extraction and alteration of fish protein from a low value source to produce highly digestible, low molecular weight peptide powders that could be used as a fortifying health ingredient, especially in beverages.
http://hdl.handle.net/10468/5001
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Characterization of protein hydrolysates from blue whiting (Micromesistius poutassou) and their application in beverage fortification
(2020)
Egerton, Sian; Culloty, Sarah; Whooley, Jason; STANTON, CATHERINE; Ross, R Paul
Characterization of protein hydrolysates from blue whiting (Micromesistius poutassou) and their application in beverage fortification
(2020)
Egerton, Sian; Culloty, Sarah; Whooley, Jason; STANTON, CATHERINE; Ross, R Paul
Abstract:
Enzymatic hydrolysis of fish proteins has been employed as a principle method for converting under-utilised fish into valuable products for the pharmaceutical and health food industries. In this study, six commercial enzymes were tested for their ability to make fish protein hydrolysate powders from whole blue whiting. The chemical and functional properties of these powders were compared. The powders all had high solubility (>80%) across a wide pH range in water and their solubility improved further within a vitamin-tea beverage matrix (>85%). Varying degrees of anti-oxidant activities were recorded for the powders using three model systems (DPPH, ferrous chelating and reducing power). This study demonstrates that commercial enzymes are useful for the extraction and alteration of fish protein from a low value source to produce highly digestible, low molecular weight peptide powders that could be used as a fortifying health ingredient, especially in beverages.
http://hdl.handle.net/11019/2024
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Choosing Healthy Eating for Infant Health (CHErIsH) study: protocol for a feasibility study
(2019)
Matvienko-Sikar, Karen; Toomey, Elaine; Queally, Michelle; Flannery, Caragh; O'Nei...
Choosing Healthy Eating for Infant Health (CHErIsH) study: protocol for a feasibility study
(2019)
Matvienko-Sikar, Karen; Toomey, Elaine; Queally, Michelle; Flannery, Caragh; O'Neill, Kate; Dinan, Timothy G.; Doherty, Edel; Harrington, Janas M.; Hayes, Catherine; Heary, Caroline; Hennessy, Marita; Kelly, Colette; Mc Hugh, Sheena M.; McSharry, Jenny; Stanton, Catherine; Heffernan, Tony; Byrne, Molly; Kearney, Patricia M.
Abstract:
Introduction: Childhood obesity is a public health challenge. There is evidence for associations between parents’ feeding behaviours and childhood obesity risk. Primary care provides a unique opportunity for delivery of infant feeding interventions for childhood obesity prevention. Implementation strategies are needed to support infant feeding intervention delivery. The Choosing Healthy Eating for Infant Health (CHErIsH) intervention is a complex infant feeding intervention delivered at infant vaccination visits, alongside a healthcare professional (HCP)-level implementation strategy to support delivery. Methods and analysis: This protocol provides a description of a non-randomised feasibility study of an infant feeding intervention and implementation strategy, with an embedded process evaluation and economic evaluation. Intervention participants will be parents of infants aged ≤6 weeks at recruitment, attending a participating HCP in a primary care practice. The intervention will b...
http://hdl.handle.net/10468/8760
Marked
Mark
Choosing Healthy Eating for Infant Health (CHErIsH) study: protocol for a feasibility study
(2020)
Matvienko-Sikar, Karen; Toomey, Elaine; Queally, Michelle; Flannery, Caragh; O Neill, K...
Choosing Healthy Eating for Infant Health (CHErIsH) study: protocol for a feasibility study
(2020)
Matvienko-Sikar, Karen; Toomey, Elaine; Queally, Michelle; Flannery, Caragh; O Neill, Kate; Dinan, Ted G; Doherty, Edel; Harrington, Janas M; Hayes, Catherine; Heary, Caroline; Hennessy, Marita; Kelly, Colette; Mc Hugh, Sheena M; McSharry, Jenny; STANTON, CATHERINE; Heffernan, Tony; Byrne, Molly; Kearney, Patricia M
Abstract:
Introduction: Cildhood obesity is a public health challenge. There is evidence for associations between parents’ feeding behaviours and childhood obesity risk. Primary care provides a unique opportunity for delivery of infant feeding interventions for childhood obesity prevention. Implementation strategies are needed to support infant feeding intervention delivery. The Choosing Healthy Eating for Infant Health (CHErIsH) intervention is a complex infant feeding intervention delivered at infant vaccination visits, alongside a healthcare professional (HCP)-level implementation strategy to support delivery. Methods and analysis: This protocol provides a description of a non-randomised feasibility study of an infant feeding intervention and implementation strategy, with an embedded process evaluation and economic evaluation. Intervention participants will be parents of infants aged ≤6 weeks at recruitment, attending a participating HCP in a primary care practice. The intervention will ...
http://hdl.handle.net/11019/1962
Marked
Mark
Choosing healthy eating for infant health (CHErIsH) study: protocol for a feasibility study
(2019)
Matvienko-Sikar, Karen; Toomey, Elaine C.; Queally, Michelle; Flannery, Caragh; O'...
Choosing healthy eating for infant health (CHErIsH) study: protocol for a feasibility study
(2019)
Matvienko-Sikar, Karen; Toomey, Elaine C.; Queally, Michelle; Flannery, Caragh; O'Neill, Kate; Dinan, Ted G.; Doherty, Edel; Harrington, Janas M.; Hayes, Catherine B.; Heary, Caroline; Hennessy, Marita; Kelly, Colette; McHugh, Sheena M.; McSharry, Jenny; Stanton, Catherine; Heffernan, Tony; Byrne, Molly; Kearney, Patricia M.
Abstract:
Introduction Childhood obesity is a public health challenge. There is evidence for associations between parents’ feeding behaviours and childhood obesity risk. Primary care provides a unique opportunity for delivery of infant feeding interventions for childhood obesity prevention. Implementation strategies are needed to support infant feeding intervention delivery. The Choosing Healthy Eating for Infant Health (CHErIsH) intervention is a complex infant feeding intervention delivered at infant vaccination visits, alongside a healthcare professional (HCP)-level implementation strategy to support delivery. Methods and analysis This protocol provides a description of a non-randomised feasibility study of an infant feeding intervention and implementation strategy, with an embedded process evaluation and economic evaluation. Intervention participants will be parents of infants aged ≤6 weeks at recruitment, attending a participating HCP in a primary care practice. The intervention will be ...
http://hdl.handle.net/10344/9441
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