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Author = Sreenan, Joseph M.;
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Displaying Results 1 - 3 of 3 on page 1 of 1
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Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ests
(2018)
Casey, Orla M.; Fitzpatrick, Richard; McInerney, James O.; Morris, Dermot G.; Powell, R...
Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ests
(2018)
Casey, Orla M.; Fitzpatrick, Richard; McInerney, James O.; Morris, Dermot G.; Powell, Richard; Sreenan, Joseph M.
http://hdl.handle.net/10379/8951
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Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ESTs
(2004)
Casey, Orla M.; Fitzpatrick, Richard; McInerney, James O.; Morris, Dermot G.; Powell, R...
Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ESTs
(2004)
Casey, Orla M.; Fitzpatrick, Richard; McInerney, James O.; Morris, Dermot G.; Powell, Richard; Sreenan, Joseph M.
Abstract:
To gain new insights into gene identity and gene expression in the bovine corpus luteum (CL) a directionally cloned CL cDNA library was constructed, screened with a total CL cDNA probe and clones representing abundant and rare mRNA transcripts isolated. The 5Vterminal DNA sequence of 960 cDNA clones, composed of 192 abundant and 768 rare mRNA transcripts was determined and clustered into 351 non-redundant expressed sequence tag (EST) groups. Bioinformatic analysis revealed that 309 (88%) of the ESTs showed significant homology to existing sequences in the protein and nucleotide public databases. Several previously unidentified bovine genes encoding proteins associated with key aspects of CL function including extracellular matrix remodelling, lipid metabolism/steroid biosynthesis and apoptosis, were identified. Forty-two (12%) of the ESTs showed homology with human or with other uncharacterised ESTs, some of these were abundantly expressed and may therefore play an important role in...
http://mural.maynoothuniversity.ie/412/
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Effect of acute nutritional restriction on incidence of anovulation and periovulatory estradiol and gonadotropin concentrations in beef heifers1
(2018)
Mackey, David R.; Sreenan, Joseph M.; Roche, James F.; Diskin, Michael G.
Effect of acute nutritional restriction on incidence of anovulation and periovulatory estradiol and gonadotropin concentrations in beef heifers1
(2018)
Mackey, David R.; Sreenan, Joseph M.; Roche, James F.; Diskin, Michael G.
Abstract:
The effects of acute nutritional restriction on follicular dynamics, incidence of anovulation, and periovulatory estradiol and gonadotropin concentrations were studied in two replicates using beef heifers exhibiting regular estrous cycles. Heifers fed a diet supplying 1.2 maintenance (1.2 Mn) were synchronized using an intravaginal progesterone-releasing device for 8 days. One day before device removal, heifers were allocated randomly, within replicate, to a diet supplying 0.4 Mn (n = 20), or kept at 1.2 Mn (n = 21). On the sixth day after detected ovulation, heifers received 500 mu g of synthetic prostaglandin F-2 alpha (PGF(2 alpha)) to induce luteolysis, estrus, and ovulation of the first dominant follicle (DF). Animals were inseminated and returned to a diet of 1.2 Mn. Pregnancy diagnosis was performed 30 days later. The maximum diameter subsequently attained by the DF present at progesterone withdrawal was smaller (P < 0.01) in heifers fed 0.4 Mn. Two heifers fed 0.4 Mn ...
http://hdl.handle.net/10379/9463
Displaying Results 1 - 3 of 3 on page 1 of 1
Bibtex
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Institution
Maynooth University (1)
NUI Galway (2)
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Peer-reviewed (1)
Unknown (2)
Year
2018 (2)
2004 (1)
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