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Author = Wills, Norma M.;
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Displaying Results 1 - 2 of 2 on page 1 of 1
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Stimulation of stop codon readthrough: frequent presence of an extended 3' RNA structural element
(2011)
Firth, Andrew E.; Wills, Norma M.; Gesteland, Raymond F.; Atkins, John F.
Stimulation of stop codon readthrough: frequent presence of an extended 3' RNA structural element
(2011)
Firth, Andrew E.; Wills, Norma M.; Gesteland, Raymond F.; Atkins, John F.
Abstract:
In Sindbis, Venezuelan equine encephalitis and related alphaviruses, the polymerase is translated as a fusion with other non-structural proteins via readthrough of a UGA stop codon. Surprisingly, earlier work reported that the signal for efficient readthrough comprises a single cytidine residue 3'-adjacent to the UGA. However, analysis of variability at synonymous sites revealed strikingly enhanced conservation within the similar to 150 nt 3'-adjacent to the UGA, and RNA folding algorithms revealed the potential for a phylogenetically conserved stem-loop structure in the same region. Mutational analysis of the predicted structure demonstrated that the stem-loop increases readthrough by up to 10-fold. The same computational analysis indicated that similar RNA structures are likely to be relevant to readthrough in certain plant virus genera, notably Furovirus, Pomovirus, Tobravirus, Pecluvirus and Benyvirus, as well as the Drosophilia gene kelch. These results suggest that 3...
http://hdl.handle.net/10468/5023
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Transcriptional frameshifting rescues Citrobacter rodentium Type VI secretion by the production of two length variants from the prematurely interrupted tssM gene
(2014)
Gueguen, Erwan; Wills, Norma M.; Atkins, John F.; Cascales, Eric
Transcriptional frameshifting rescues Citrobacter rodentium Type VI secretion by the production of two length variants from the prematurely interrupted tssM gene
(2014)
Gueguen, Erwan; Wills, Norma M.; Atkins, John F.; Cascales, Eric
Abstract:
The Type VI secretion system (T6SS) mediates toxin delivery into both eukaryotic and prokaryotic cells. It is composed of a cytoplasmic structure resembling the tail of contractile bacteriophages anchored to the cell envelope through a membrane complex composed of the TssL and TssM inner membrane proteins and of the TssJ outer membrane lipoprotein. The C-terminal domain of TssM is required for its interaction with TssJ, and for the function of the T6SS. In Citrobacter rodentium, the tssM1 gene does not encode the C-terminal domain. However, the stop codon is preceded by a run of 11 consecutive adenosines. In this study, we demonstrate that this poly-A tract is a transcriptional slippery site that induces the incorporation of additional adenosines, leading to frameshifting, and hence the production of two TssM1 variants, including a full-length canonical protein. We show that both forms of TssM1, and the ratio between these two forms, are required for the function of the T6SS in C. r...
http://hdl.handle.net/10468/2319
Displaying Results 1 - 2 of 2 on page 1 of 1
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