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Limited By:	Subject = 3D bioprinting;

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3D bioprinting of cartilage-mimetic implants for biological joint resurfacing (2020)

SCHIPANI, ROSSANA

3D bioprinting of cartilage-mimetic implants for biological joint resurfacing (2020)

SCHIPANI, ROSSANA

Abstract:

A major challenge in the field of tissue engineering and regenerative medicine is the development of effective therapies for treating large cartilage or osteochondral defects and ultimately regenerating whole osteoarthritic joints. The objective of this thesis was to 3D bioprint cell-laden biomaterials with biomimetic mechanical properties as implants for regenerating large osteochondral defects. To this end, a finite element modelling (FEM) strategy was first developed to design the 3D printed polycaprolactone (PCL) networks with user-defined mechanical properties. These PCL networks were then combined with an alginate-gelatin methacryloyl (gelMA) interpenetrating network (IPN) hydrogel to develop 3D bioprinted constructs that were both mechanically functional and supportive of mesenchymal stromal cells (MSCs) chondrogenesis. When the IPN hydrogels were reinforced with a PCL network characterized by relatively high tension-compression nonlinearity, the resulting composites possesse...

http://hdl.handle.net/2262/93022

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3D Bioprinting of Developmentally Inspired Templates for Bone and Joint Regeneration (2018)

Abstract:

There is an urgent need to develop alternatives to synthetic joint prosthesis to promote the regeneration of diseased osteoarthritic joints. Cell based therapies have shown promise for repairing cartilage and bone; however, existing approaches are designed to repair small focal defects, and are not suitable for treating large injuries or for regenerating osteoarthritic joints. The objective of this thesis was to bioprint cell laden constructs capable of recapitulating key aspects of limb development as implants for large bone defect healing and joint regeneration. To this end, a novel biofabrication strategy for engineering whole bone organs was first developed by bioprinting hypertrophic cartilage templates with the capacity to undergo endochondral ossification following implantation in vivo. These soft cartilaginous templates could be mechanically reinforced with a network of co-printed polycaprolactone (PCL) microfibers, resulting in a dramatic increase in construct compressive ...

http://hdl.handle.net/2262/82727

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3D-Printed Peptide-Hydrogel Nanoparticle Composites for Surface-Enhanced Raman Spectroscopy Sensing (2019)

Almohammed, Sawsan; Alruwaili, Maha; Reynaud, Emmanuel G.; Redmond, Gareth; Rice, James...

3D-Printed Peptide-Hydrogel Nanoparticle Composites for Surface-Enhanced Raman Spectroscopy Sensing (2019)

Almohammed, Sawsan; Alruwaili, Maha; Reynaud, Emmanuel G.; Redmond, Gareth; Rice, James H.; Rodriguez, Brian J.

Abstract:

Precise control over the arrangement of plasmonic nanomaterials is critical for label-free single-molecule surface-enhanced Raman spectroscopy (SERS)-based sensing applications. SERS templates should provide high sensitivity and reproducibility and be cost-effective and easy to prepare. Additive manufacturing by extrusion-based three-dimensional (3D) printing is an emerging technique for the spatial arrangement of nanomaterials and is a method that may satisfy these SERS template requirements. In this work, we use 3D printing to produce sensitive and reproducible SERS templates using a fluorenylmethyloxycarbonyl diphenylalanine (Fmoc-FF) hydrogel loaded with silver or gold nanoparticles. The Fmoc-FF template allows the detection of low Raman cross-section molecules such as adenine at concentrations as low as 100 pM.
Enterprise Ireland
European Commission Horizon 2020
Science Foundation Ireland
The Ministry of Higher Education of Saudi Arabia under the King Abdullah S...

http://hdl.handle.net/10197/10962

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Liquid-phase 3D bioprinting of gelatin alginate hydrogels: influence of printing parameters on hydrogel line width and layer height (2019)

Alruwaili, Maha; Lopez, Jose A.; McCarthy, Kevin; Reynaud, Emmanuel G.; Rodriguez, Bria...

Liquid-phase 3D bioprinting of gelatin alginate hydrogels: influence of printing parameters on hydrogel line width and layer height (2019)

Alruwaili, Maha; Lopez, Jose A.; McCarthy, Kevin; Reynaud, Emmanuel G.; Rodriguez, Brian J.

Abstract:

Extrusion-based 3D bioprinting is a direct deposition approach used to create three-dimensional (3D) tissue scaffolds typically comprising hydrogels. Hydrogels are hydrated polymer networks that are chemically or physically cross-linked. Often, 3D bioprinting is performed in air, despite the hydrated nature of hydrogels and the potential advantage of using a liquid phase to provide cross-linking and otherwise functionalize the hydrogel. In this work, we print gelatin alginate hydrogels directly into a cross-linking solution of calcium chloride and investigate the influence of nozzle diameter, distance between nozzle and surface, calcium chloride concentration, and extrusion rate on the dimensions of the printed hydrogel. The hydrogel layer height was generally found to increase with increasing extrusion rate and nozzle distance, according to the increased volume extruded and the available space, respectively. In addition, the hydrogel width was generally found to increase with decre...

http://hdl.handle.net/10197/10925

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Reinforcing interpenetrating network hydrogels with 3D printed polymer networks to engineer cartilage mimetic composites (2020)

Kelly, Daniel

Reinforcing interpenetrating network hydrogels with 3D printed polymer networks to engineer cartilage mimetic composites (2020)

Kelly, Daniel

Abstract:

Engineering constructs that mimic the complex structure, composition and biomechanics of the articular cartilage represents a promising route to joint regeneration. Such tissue engineering strategies require the development of biomaterials that mimic the mechanical properties of articular cartilage whilst simultaneously providing an environment supportive of chondrogenesis. Here three-dimensional (3D) bioprinting is used to develop polycaprolactone (PCL) fibre networks to mechanically reinforce interpenetrating network (IPN) hydrogels consisting of alginate and gelatin methacryloyl (GelMA). Inspired by the significant tension-compression nonlinearity of the collagen network in articular cartilage, we printed reinforcing PCL networks with different ratios of tensile to compressive modulus. Synergistic increases in compressive modulus were observed when IPN hydrogels were reinforced with PCL networks that were relatively soft in compression and stiff in tension. The resulting composit...

http://hdl.handle.net/2262/93558

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The Development of a Novel 3D Bioprinting Strategy to Drive Vascularisation, and its Application for Bone Tissue Engineering (2020)

NULTY, JESSICA

The Development of a Novel 3D Bioprinting Strategy to Drive Vascularisation, and its Application for Bone Tissue Engineering (2020)

NULTY, JESSICA

Abstract:

One of the major challenges facing the field of tissue engineering today is vascularisation. Without a system in place to circulate oxygen, nutrients and metabolites, the size of many tissue engineered constructs is restricted, as embedded cells must rely on the diffusion of these factors for their survival. This has limited the clinical translation of engineered tissues and organs. The inclusion of a vascular network within tissue engineered constructs could overcome this constraint, thereby enabling the scaling up of such tissues to previously unattainable, clinically relevant sizes. One branch of tissue engineering which has a considerable need for more effective vascularisation strategies is the field of bone tissue engineering. Bone tissue engineering endeavours to produce bone substitutes and provide a viable alternative to autologous bone grafts. To achieve this, new approaches to produce large, viable bone tissues must be identified. The overall aim of this thesis was to dev...

http://hdl.handle.net/2262/93000

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Towards 3D bioprinting of anatomically accurate, mechanically reinforced cartilage templates for biological joint resurfacing (2018)

CRITCHLEY, SUSAN

Towards 3D bioprinting of anatomically accurate, mechanically reinforced cartilage templates for biological joint resurfacing (2018)

CRITCHLEY, SUSAN

Abstract:

Osteoarthritis (OA) is a pervasive disease worldwide which affects the articular cartilage and the underlying bone in synovial joints such as the knee. Currently, the only treatment for a severely degenerated knee joint is a total or partial joint replacement with a metal and polymer prosthesis. Whilst these procedures are well established, failures are not uncommon resulting in a more complicated revision surgery. The aging worldwide population and the increase in the instances of younger patients being diagnosed with OA are primary motivations behind the pursuit of new treatment options. Tissue engineering approaches have been gaining traction in recent years, having being successfully translated to the clinic to treat small focal defects. These therapies combine cells, scaffolds and signalling molecules to drive tissue formation and maturation to regenerate damaged tissues. 3D printing technology can be used in tandem with tissue engineering strategies to fabricate constructs tha...

http://hdl.handle.net/2262/82805

Displaying Results 1 - 7 of 7 on page 1 of 1