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Subject = Cervico-vaginal mucus;
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Displaying Results 1 - 2 of 2 on page 1 of 1
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Cervico-vaginal mucus (CVM) – an accessible source of immunologically informative biomolecules
(2020)
Adnane, Mounir; Meade, Kieran G; O’Farrelly, Cliona
Cervico-vaginal mucus (CVM) – an accessible source of immunologically informative biomolecules
(2020)
Adnane, Mounir; Meade, Kieran G; O’Farrelly, Cliona
Abstract:
Cervico-vaginal mucus (CVM), the product of epithelial cells lining the uterus, cervix and vagina, is secreted to facilitate uterine lubrication and microbial clearance. Predominantly composed of water and mucins, CVM also contains high levels of immuno-active proteins such as immunoglobulin A (IgA), lactoferrin and lysozyme which protect against infection by blocking adhesion and mediating microbial killing. The repertoire of cytokines, chemokines and antimicrobial peptides is predominantly generated by the secretions of endometrial epithelial cells into the uterine lumen and concentrated in the CVM. The quantity and relative proportions of these inflammatory biomarkers are affected by diverse factors including the estrus cycle and health status of the animal and therefore potentially provide important diagnostic and prognostic indicators. We propose that measuring molecular signatures in bovine CVM could be a useful approach to identifying and monitoring genital tract pathologies ...
http://hdl.handle.net/11019/2305
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Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle
(2018)
Adnane, Mounir; Kelly, Paul M; Chapwanya, Aspinas; Meade, Kieran G; O'Farrelly, Cl...
Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle
(2018)
Adnane, Mounir; Kelly, Paul M; Chapwanya, Aspinas; Meade, Kieran G; O'Farrelly, Cliona
Abstract:
Background In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved method for processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and α1-acid glycoprotein (AGP) were measured by ELISA. Results We found that use of reducing agents to ...
http://hdl.handle.net/11019/1603
Displaying Results 1 - 2 of 2 on page 1 of 1
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