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Subject = Cervico-vaginal mucus (CVM);
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Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle
(2018)
O'Farrelly, Cliona; Adnane, Mounir; Kelly, Paul; Chapwanya, Aspinas; Meade, Kieran
Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle
(2018)
O'Farrelly, Cliona; Adnane, Mounir; Kelly, Paul; Chapwanya, Aspinas; Meade, Kieran
Abstract:
Background: In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved methodfor processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and?1-acid glycoprotein (AGP) were measured by ELISA. Results: We found that use of reducing agents to liquefy CVM affects protein yield and the accuracy of biomarker detection. Our...
http://hdl.handle.net/2262/89581
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