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Limited By:	Subject = Endometritis;

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Displaying Results 1 - 5 of 5 on page 1 of 1

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Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle (2018)

Adnane, Mounir; Kelly, Paul M; Chapwanya, Aspinas; Meade, Kieran G; O'Farrelly, Cl...

Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle (2018)

Adnane, Mounir; Kelly, Paul M; Chapwanya, Aspinas; Meade, Kieran G; O'Farrelly, Cliona

Abstract:

Background In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved method for processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and α1-acid glycoprotein (AGP) were measured by ELISA. Results We found that use of reducing agents to ...

http://hdl.handle.net/11019/1603

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Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle (2018)

O'Farrelly, Cliona; Adnane, Mounir; Kelly, Paul; Chapwanya, Aspinas; Meade, Kieran

Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle (2018)

O'Farrelly, Cliona; Adnane, Mounir; Kelly, Paul; Chapwanya, Aspinas; Meade, Kieran

Abstract:

Background: In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved methodfor processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and?1-acid glycoprotein (AGP) were measured by ELISA. Results: We found that use of reducing agents to liquefy CVM affects protein yield and the accuracy of biomarker detection. Our...

http://hdl.handle.net/2262/89581

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Integrated analysis of the local and systemic changes preceding the development of post-partum cytological endometritis. (2015)

O'FARRELLY, CLIONA

Integrated analysis of the local and systemic changes preceding the development of post-partum cytological endometritis. (2015)

O'FARRELLY, CLIONA

Abstract:

BACKGROUND: The regulation of endometrial inflammation has important consequences for the resumption of bovine fertility postpartum. All cows experience bacterial influx into the uterus after calving; however a significant proportion fail to clear infection leading to the development of cytological endometritis (CE) and compromised fertility. We hypothesised that early immunological changes could not only act as potential prognostic biomarkers for the subsequent development of disease but also shed light on the pathogenesis of endometritis in the postpartum dairy cow. METHODS: Endometrial biopsy RNA was extracted from 15 cows at 7 and 21 days postpartum (DPP), using the Qiagen RNeasy(?) Plus Mini kit and quality determined using an Agilent 2100 bioanalyser. Disease status was determined by histpathology based on inflammatory cell infiltrate. RNA-seq of both mRNA and miRNA libraries were performed on an Illumina? HiSeq(?) 2000. Paired reads were aligned to the bovine genome with Bo...

http://hdl.handle.net/2262/77381

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Non-canonical inflammasome-mediated IL-1?? production by primary endometrial epithelial and stromal fibroblast cells is NLRP3 and caspase-4 dependent (2019)

O'Farrelly, Cliona; Kelly, Paul; Meade, Kieran G.

Non-canonical inflammasome-mediated IL-1?? production by primary endometrial epithelial and stromal fibroblast cells is NLRP3 and caspase-4 dependent (2019)

O'Farrelly, Cliona; Kelly, Paul; Meade, Kieran G.

Abstract:

Inflammation of the post-partum uterus is a normal physiological event, crucial for tissue involution and repair. However, in the bovine, some cows fail to resolve this inflammation, resulting in endometritis, which compromises fertility. Earlier work has identified upregulated expression of the potent inflammatory cytokine IL-1? early post-partum, in cows which subsequently develop endometritis. As a result, targeting IL-1? expression holds potential as a novel treatment for this disease, yet the regulatory mechanisms contributing to IL-1? expression in the bovine endometrium remain unknown. To investigate this, endometrial tissue samples were obtained 7 and 21 days post-partum (DPP) from cows that were diagnosed with endometritis at 21 DPP and cows that experienced a physiological level of inflammation throughout involution. IL-1? was measured by qPCR, ELISA, and immunohistochemistry. Seven DPP, endometrial IL-1? protein levels were significantly higher in animals that proceeded t...

http://hdl.handle.net/2262/89548

Marked Mark

Non-canonical Inflammasome-Mediated IL-1β Production by Primary Endometrial Epithelial and Stromal Fibroblast Cells Is NLRP3 and Caspase-4 Dependent (2020)

Kelly, Paul; Meade, Kieran G; O'Farrelly, Cliona

Non-canonical Inflammasome-Mediated IL-1β Production by Primary Endometrial Epithelial and Stromal Fibroblast Cells Is NLRP3 and Caspase-4 Dependent (2020)

Kelly, Paul; Meade, Kieran G; O'Farrelly, Cliona

Abstract:

Inflammation of the post-partum uterus is a normal physiological event, crucial for tissue involution and repair. However, in the bovine, some cows fail to resolve this inflammation, resulting in endometritis, which compromises fertility. Earlier work has identified upregulated expression of the potent inflammatory cytokine IL-1β early post-partum, in cows which subsequently develop endometritis. As a result, targeting IL-1β expression holds potential as a novel treatment for this disease, yet the regulatory mechanisms contributing to IL-1β expression in the bovine endometrium remain unknown. To investigate this, endometrial tissue samples were obtained 7 and 21 days post-partum (DPP) from cows that were diagnosed with endometritis at 21 DPP and cows that experienced a physiological level of inflammation throughout involution. IL-1β was measured by qPCR, ELISA, and immunohistochemistry. Seven DPP, endometrial IL-1β protein levels were significantly higher in animals that proceeded t...

http://hdl.handle.net/11019/2114

Displaying Results 1 - 5 of 5 on page 1 of 1