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Displaying Results 1 - 25 of 214 on page 1 of 9
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A Microfluidic Anti-Factor Xa Assay Device for Point-of-Care Monitoring of Anticoagulation Therapy
(2013)
Harris, Leanne F; Rainey, Paul; Castro-Lopez, Vanessa; O'Donnell, James S; Killard...
A Microfluidic Anti-Factor Xa Assay Device for Point-of-Care Monitoring of Anticoagulation Therapy
(2013)
Harris, Leanne F; Rainey, Paul; Castro-Lopez, Vanessa; O'Donnell, James S; Killard, Anthony J
Abstract:
The development of new point-of-care coagulation assay devices is necessary due to the increasing number of patients requiring long-term anticoagulation in addition to the desire for appropriate, targeted anticoagulant therapy and a more rapid response to optimization of treatment. However, the majority of point-of-care devices currently available for hemostasis testing rely on clot-based endpoints which are variable and unreliable and are limited to measuring only certain portions of the coagulation pathway. There is a need for the introduction of point-of-care devices that can execute a broader range of tests; for example, the diagnosis of factor-specific diseases, as well as more reliable assays of anticoagulant status, particularly in response to the emergence of new anticoagulant drugs. Here we present a novel fluorescence-based anti-Factor Xa (FXa) microfluidic assay device for monitoring the effect of anticoagulant therapy at the point-of-care. The device is a disposable, lam...
https://arrow.dit.ie/scschbioart/147
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A Novel Microfluidic Anti-Factor Xa Assay Device for Monitoring Anticoagulant Therapy at the Point-Of-Care
(2013)
Harris, Leanne; Rainey, Paul; Castro-Lopez, Vanessa; O'Donnell, James S; Killard, ...
A Novel Microfluidic Anti-Factor Xa Assay Device for Monitoring Anticoagulant Therapy at the Point-Of-Care
(2013)
Harris, Leanne; Rainey, Paul; Castro-Lopez, Vanessa; O'Donnell, James S; Killard, Anthony J
Abstract:
Millions of patients worldwide are receiving anticoagulant therapy to treat hypercoagulable diseases. While standard testing is still performed in the central laboratory, point-of-care (POC) diagnostics are being developed due to the increasing number of patients requiring long-term anticoagulation and with a need for more personalized and targeted therapy. Many POC devices on the market focus on clot measurement, a technique which is limited in terms of variability, highlighting the need for more reliable assays of anticoagulant status. The anti-Xa assay, a factor specific optical assay, was developed to measure the extent to which exogenous factor Xa (FXa) is inhibited by heparinantithrombin complexes. We have developed a novel microfluidic device and assay for monitoring the effect of heparin anticoagulant therapy at the point-of-care. The assay which was also developed in our institute is based on the anti-Xa assay principle but uses fluorescence as the method of detection. Our ...
https://arrow.dit.ie/scschbioart/146
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A novel platinum complex of the histone deacetylase inhibitor belinostat: rational design, development and in vitro cytotoxicity.
(2013)
Parker, James P; Nimir, Hassan; Griffith, Darren M; Duff, Brian; Chubb, Anthony J; Bren...
A novel platinum complex of the histone deacetylase inhibitor belinostat: rational design, development and in vitro cytotoxicity.
(2013)
Parker, James P; Nimir, Hassan; Griffith, Darren M; Duff, Brian; Chubb, Anthony J; Brennan, Marian P; Morgan, Maria; Egan, Denise; Marmion, Celine
Abstract:
<p>NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Inorganic Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Inorganic Biochemistry. 2013;124:70-7.</p> <p>DOI 10.1016/j.jinorgbio.2013.03.011</p> <p><a href="http://www.sciencedirect.com/science/article/pii/S016201341300069X" target="_blank">http://www.sciencedirect.com/science/article/pii/S016201341300069X</a></p>
<p>The successful design and synthesis of a novel Pt complex of the histone deacteylase inhibitor belinostat are reported. Molecular modelling assisted in the identification of a suitable malonate derivative o...
https://epubs.rcsi.ie/mctart/66
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A novel role for the fibrinogen Asn-Gly-Arg (NGR) motif in platelet function.
(2014)
Moriarty, Róisín; McManus, Ciara A; Lambert, Matthew; Tilley, Thea; Devocelle, Marc; Br...
A novel role for the fibrinogen Asn-Gly-Arg (NGR) motif in platelet function.
(2014)
Moriarty, Róisín; McManus, Ciara A; Lambert, Matthew; Tilley, Thea; Devocelle, Marc; Brennan, Marian; Kerrigan, Steven W; Cox, Dermot
Abstract:
<p>This article is not an exact copy of the original published article in Thrombosis and Haemostasis. The definitive publisher-authenticated version of Moriarty R, McManus CA, Lambert M, Tiley T, Devocelle M, Brennan M, Kerrigan W, Cox D. A novel role for the fibrinogen Asn-Gly-Arg (NGR) motif in platelet function. Thrombosis and Haemostasis. 2014;113(2) is available online at: <a href="http://th.schattauer.de/en/contents/archive/issue/special/manuscript/23713.html">http://th.schattauer.de/en/contents/archive/issue/special/manuscript/23713.html</a></p>
<p>The integrin αIIbβ3 on resting platelets can bind to immobilised fibrinogen resulting in platelet spreading and activation but requires activation to bind to soluble fibrinogen. αIIbβ3 is known to interact with the general integrin-recognition motif RGD (arginine-glycine-aspartate) as well as the fibrinogen-specific γ-chain dodecapeptide; however, it is not known how fibrinogen binding tr...
https://epubs.rcsi.ie/mctart/67
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A Peroxisomal Acyltransferase in Mouse Identifies a Novel Pathway for Taurine Conjugation of Fatty Acids.
(2007)
Reilly, Sarah-Jayne; O'Shea, Eithne; James, O'Byrne; Alexson, Stefan; Hunt, Mary
A Peroxisomal Acyltransferase in Mouse Identifies a Novel Pathway for Taurine Conjugation of Fatty Acids.
(2007)
Reilly, Sarah-Jayne; O'Shea, Eithne; James, O'Byrne; Alexson, Stefan; Hunt, Mary
Abstract:
A wide variety of endogenous carboxylic acids and xenobiotics are conjugated with amino acids, before excretion in urine or bile. The conjugation of carboxylic acids and bile acids with taurine and glycine has been widely characterized and de-novo synthesized bile acids are conjugated to either glycine or taurine in peroxisomes. Peroxisomes are also involved in the oxidation of several other lipid molecules, such as very long chain acyl-CoAs, branched chain acyl-CoAs and prostaglandins. In this study we have now identified a novel peroxisomal enzyme called acyl-CoA:amino acid N-acyltransferase (ACNAT1). Recombinantly expressed ACNAT1 acts as an acyltransferase that efficiently conjugates very long-chain and long-chain fatty acids to taurine. The enzyme shows no conjugating activity with glycine, showing that it is a specific taurine conjugator. Acnat1 is mainly expressed in liver and kidney and the gene is localized in a gene cluster, together with two further acyltransferases, one ...
https://arrow.dit.ie/scschbioart/13
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A polymorphism in ACE2 is associated with a lower risk for fatal cardiovascular events in females: the MORGAM project.
(2011)
Vangjeli, Ciara; Dicker, Patrick; Tregouet, David-Alexandre; Shields, Denis C; Evans, A...
A polymorphism in ACE2 is associated with a lower risk for fatal cardiovascular events in females: the MORGAM project.
(2011)
Vangjeli, Ciara; Dicker, Patrick; Tregouet, David-Alexandre; Shields, Denis C; Evans, Alun; Stanton, Alice V
Abstract:
<p>Angiotensin II, a vasoconstrictor and the main effector molecule of the renin-angiotensin system, is known to influence inflammation, thrombosis, low-density lipoprotein oxidation and growth factors, all of which contribute to cardiovascular disease. The associations of polymorphisms in the angiotensin-converting enzyme 2 (ACE2) gene with cardiovascular risk have not been fully determined. Single nucleotide polymorphisms (SNPs) in ACE2 were genotyped in participants of the prospective MORGAM study (n = 5092) from five cohorts: ATBC, FINRISK, Northern Sweden, PRIME/Belfast and PRIME/France. Using a case-cohort design, associations were sought between SNPs and haplotypes with cardiovascular events during follow-up (Cox proportional hazards model). The comparison group were a subset of all MORGAM participants who were selected to ensure similar age and sex distributions among the cases and controls. The A allele of the rs2285666 SNP (HR = 0.3, p = 0.04) was significantly assoc...
https://epubs.rcsi.ie/mctart/49
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A Roadmap for navigating the Life Sciences Linked Open Data Cloud
(2015)
Hasnain, Ali; Sana e Zainab, Syeda; Kamdar, Maulik; Mehmood, Qaiser; Deus, Helena; Mehd...
A Roadmap for navigating the Life Sciences Linked Open Data Cloud
(2015)
Hasnain, Ali; Sana e Zainab, Syeda; Kamdar, Maulik; Mehmood, Qaiser; Deus, Helena; Mehdi, Muntazir; Decker, Stefan
Abstract:
Conference paper
Multiple datasets that add high value to biomedical research have been exposed on the web as a part of the Life Sciences Linked Open Data (LSLOD) Cloud. The ability to easily navigate through these datasets is crucial for personalized medicine and the improvement of drug discovery process. However, navigating these multiple datasets is not trivial as most of these are only available as isolated SPARQL endpoints with very little vocabulary reuse. The content that is indexed through these endpoints is scarce, making the indexed dataset opaque for users. In this paper, we propose an approach for the creation of an active Linked Life Sciences Data Roadmap, a set of con gurable rules which can be used to discover links (roads) between biological entities (cities) in the LSLOD cloud. We have catalogued and linked concepts and properties from 137 public SPARQL endpoints. Our Roadmap is primarily used to dynamically assemble queries retrieving data from multiple SPARQL ...
http://hdl.handle.net/10379/4846
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A role for intravenous immunoglobulin in the treatment of Acquired Von Willebrand Syndrome associated with IgM gammopathy.
(2018)
Lavin, Michelle; Ryan, Kevin; White, Barry; Byrne, Mary; O'Connell, Niamh M; O...
A role for intravenous immunoglobulin in the treatment of Acquired Von Willebrand Syndrome associated with IgM gammopathy.
(2018)
Lavin, Michelle; Ryan, Kevin; White, Barry; Byrne, Mary; O'Connell, Niamh M; O'Donnell, James S
Abstract:
<p>This is the peer reviewed version of the following article: Lavin M, Ryan K, White B, Byrne M, O'Connell NM, O'Donnell JS. A role for intravenous immunoglobulin in the treatment of Acquired Von Willebrand Syndrome associated with IgM gammopathy. Haemophilia. 2018;24(1):e22-e25, which has been published in final form at doi: 10.1111/hae.13374. This article may be used for non-commercial purposes in accordance with <a href="http://olabout.wiley.com/WileyCDA/Section/id-828039.html#terms">Wiley Terms and Conditions for Self-Archiving</a>."<em></em></p>
<p>Acquired Von Willebrand Syndrome (AVWS) is a rare bleeding disorder resulting from reduction in plasma von Willebrand Factor (VWF) in patients with no previous personal or family history of Von Willebrand Disease (VWD). AVWS may present with significant bleeding, complicated by short-lived responses to DDAVP or VWF-containing concentrate. AVWS is commonly associa...
https://epubs.rcsi.ie/mctart/93
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A Study Examining the Effects of Tissue Processing on Human Tissue Sections using Vibrational Spectroscopy
(2005)
O'Faolain, Eoghan; Hunter, Mary; Byrne, Joe; Kellehan, Peter; McNamara, Mary; Byrn...
A Study Examining the Effects of Tissue Processing on Human Tissue Sections using Vibrational Spectroscopy
(2005)
O'Faolain, Eoghan; Hunter, Mary; Byrne, Joe; Kellehan, Peter; McNamara, Mary; Byrne, Hugh; Lyng, Fiona
Abstract:
The use of vibrational spectroscopy in the detection of cancer is a newly emerging diagnostic field, which has shown great potential to date. Many investigations have been carried out on frozen tissue samples, which by their very nature are hard to obtain. However, histology departments have archives of thousands of tissue samples, preserved and mounted in wax blocks. If this archival material can be shown to yield good Raman and IR spectra capable of differentiating between normal and cancerous tissue, it would improve the diagnostic capabilities of spectroscopy even further. Results from these formalin-fixed paraffin processed (FFPP) tissue sections, will give a better understanding of the effects of processing and could unlock the potential diagnostic capabilities of FFPP sections. This study investigated the effect of freezing, formalin fixation, wax embedding and de-waxing. Spectra were recorded from parallel tissue sections of placenta to examine biochemical changes before, du...
https://arrow.dit.ie/materart/13
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A study of the genotoxic implications and enhancement technologies for solar disinfection (SODIS) of drinking water
(2009)
Ubomba-Jaswa, Eunice M
A study of the genotoxic implications and enhancement technologies for solar disinfection (SODIS) of drinking water
(2009)
Ubomba-Jaswa, Eunice M
Abstract:
<p>Solar disinfection (SODIS) is a household water treatment method that uses sunlight to inactivate pathogens in water. The work presented in this thesis aimed to show that SODIS is an<strong> </strong>appropriate, effective and acceptable intervention against waterborne disease. This was demonstrated by the inactivation of a highly infectious bacterial pathogen, increasing the volume of SODIS treatedwater using solar continuous-flow reactors, enhancing SODIS during sunny, cloudy and turbid water conditions by the use of compound parabolicconcentrator (CPC) mirrors and finally determining the genotoxicity of SODIS water.</p> <p>This project identified the inactivation kinetics of <em>E. coli<strong> </strong></em>0157 under simulated light by following a natural temperature profile which would be comparable to inactivation under real sunlight conditions.</p> <p>The use of CPC mirrors proved to be enhancement to SODIS...
https://epubs.rcsi.ie/phdtheses/12
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A Walk Down the Red Carpet: Students as Producers of Digital Video-Based Knowledge.
(2013)
Ryan, Barry
A Walk Down the Red Carpet: Students as Producers of Digital Video-Based Knowledge.
(2013)
Ryan, Barry
Abstract:
Disengaged and apathetic students are common in many undergraduate classrooms. Learning to these students is a passive process, typified by a consumer-like attitude. One approach to engage students, and enhance the learning experience, is to integrate active learning into the curriculum. The purpose of the pedagogical evaluative study described here was to investigate if student researched, designed and created digital video could act as a viable reusable peer learning resource. Although the use and integration of technology was central to the scope of this project, other ideas such as threshold concepts, the requirement for both active and authentic social constructivist learning, and student empowerment are pivotal to the rationalisation of this research. Overall, students appreciated an alternative method of learning; however, they were more reserved about their perceived learning and the usefulness of the peer generated videos as learning resources. This pedagogical evaluative s...
https://arrow.dit.ie/schfsehart/164
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Academic staff perspectives on delivering a shared undergraduate medical module on three transnational campuses: Practical considerations and lessons learned.
(2019)
Morgan, Maria P; Thomas, Warren; Rashid-Doubell, Fiza
Academic staff perspectives on delivering a shared undergraduate medical module on three transnational campuses: Practical considerations and lessons learned.
(2019)
Morgan, Maria P; Thomas, Warren; Rashid-Doubell, Fiza
Abstract:
<p>The Version of Scholarly Record of this Article is published in Medical Teacher. 2019 available online at: <a href="http://tandfonline.com/">http://www.tandfonline.com/</a> DOI: <a href="https://doi.org/10.1080/0142159X.2019.1649380" target="_blank">10.1080/0142159X.2019.1649380</a></p>
The Royal College of Surgeons in Ireland (RCSI) was among the first medical institutions to establish a global education community which now provides high-quality transnational health professions education aligned across three locations: Europe, the Middle East and South-East Asia. The successful implementation of a shared modularized curriculum in this context can be complex and challenging. Here we describe our insights, gained from a decade of working together as shared module Academic Leads to deliver a system-based medical module to an international student cohort. The themes covered are some of the areas where we consider ...
https://epubs.rcsi.ie/mctart/134
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Accurately assessing the risk of schizophrenia conferred by rare copy-number variation affecting genes with brain function.
(2010)
Raychaudhuri, Soumya; Korn, Joshua M; McCarroll, Steven A A; The International Schizoph...
Accurately assessing the risk of schizophrenia conferred by rare copy-number variation affecting genes with brain function.
(2010)
Raychaudhuri, Soumya; Korn, Joshua M; McCarroll, Steven A A; The International Schizophrenia Consortium; Altshuler, David; Sklar, Pamela; Purcell, Shaun; Daly, Mark J
Abstract:
<p>John Waddington, Department of Molecular and Cellular Therapeutics, RCSI is a member of The International Schizophrenia Consortium.<br />This article is also available at http://www.plosgenetics.org/.</p>
<p>Investigators have linked rare copy number variation (CNVs) to neuropsychiatric diseases, such as schizophrenia. One hypothesis is that CNV events cause disease by affecting genes with specific brain functions. Under these circumstances, we expect that CNV events in cases should impact brain-function genes more frequently than those events in controls. Previous publications have applied "pathway" analyses to genes within neuropsychiatric case CNVs to show enrichment for brain-functions. While such analyses have been suggestive, they often have not rigorously compared the rates of CNVs impacting genes with brain function in cases to controls, and therefore do not address important confounders such as the large size of brain genes and...
https://epubs.rcsi.ie/mctart/35
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Acquired factor xiii deficiency: An uncommon but easily missed cause of severe bleeding.
(2018)
Fogarty, Helen; Byrne, Mary; O'Connell, Niamh M; Ryan, Kevin; White, Barry; O'...
Acquired factor xiii deficiency: An uncommon but easily missed cause of severe bleeding.
(2018)
Fogarty, Helen; Byrne, Mary; O'Connell, Niamh M; Ryan, Kevin; White, Barry; O'Donnell, James S; Lavin, Michelle
Abstract:
<p>The original article is available at <a href="http://imj.ie/">www.imj.ie</a></p>
<p>Factor XIII (FXIII) is a plasma clotting protein involved in clot stabilization. Severe FXIII deficiency may present with severe, even fatal bleeding. Critically however, routine coagulation assays may be normal and only specific FXIII assays will detect the abnormality. Herein we discuss a case report of a patient with acquired FXIII deficiency in order to highlight the clinical challenges associated with establishing the diagnosis and discuss the treatment approach. A 70-year-old man presented with a gluteal haematoma despite no preceding personal history of bleeding. Extensive initial haemostatic investigations were normal until a specific FXIII assay showed a marked reduction in FXIII levels. With directed treatment, bleeding episodes ceased and remission was achieved. Clinical awareness of FXIII deficiency is important, so appropriate testing can be...
https://epubs.rcsi.ie/mctart/110
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ADAM22 as a predictive marker for endocrine resistant metastatic breast cancer and an LGI1 mimetic as a companion therapeutic
(2018)
Doherty, Ben
ADAM22 as a predictive marker for endocrine resistant metastatic breast cancer and an LGI1 mimetic as a companion therapeutic
(2018)
Doherty, Ben
Abstract:
<p>Approximately 70 % of breast cancer patients are classified as oestrogen receptor positive. While initial prognosis is favourable, the risk of recurrence remains long after diagnosis. Furthermore, the ability to treat recurrent tumours becomes harder over time as resistance to endocrine therapy develops. This resistant phenotype is caused in part by overexpression of the nuclear receptor co-activator SRC-1. Moreover, SRC-1 has been shown to promote metastatic development in several breast cancer models.</p> <p>Previously, our lab identified the neuronal protein ADAM22 as an SRC-1 target gene involved in endocrine resistant breast cancer metastases. Here, this metastatic role was further characterised through knockdown, knockout and overexpression studies along with a high throughput proteomic study. This study demonstrates the potential of ADAM22 as a biomarker for predicting metastatic development in endocrine resistant patients. Finally, an ADAM22 targeting pe...
https://epubs.rcsi.ie/phdtheses/231
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Aiding the Data Integration in Medicinal Settings by Means of Semantic Technologies
(2009)
Nováček, Vít; Handschuh, Siegfried
Aiding the Data Integration in Medicinal Settings by Means of Semantic Technologies
(2009)
Nováček, Vít; Handschuh, Siegfried
Abstract:
The paper introduces basic features of a novel ontology integration framework that explicitly takes the dynamics and data-intensiveness of many practical application scenarios into account. We motivate our research partially by the needs of bio-medicine scenarios that have been recently identified within the search for semantics-enabled solutions. In this context, we show a concrete example of the integration process in the life-sciences settings. Moreover, we elaborate a possible bio-medicine industry application domain of the presented framework and explain the benefits of the proposed semantic solution.
http://hdl.handle.net/10379/566
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Aldosterone-mediated apical targeting of ENaC subunits is blunted in rats with streptozotocin-induced diabetes mellitus.
(2008)
O'Neill, Heidi; Lebeck, Janne; Collins, Patrick B; Kwon, Tae-Hwan; Frøkiaer, Jørge...
Aldosterone-mediated apical targeting of ENaC subunits is blunted in rats with streptozotocin-induced diabetes mellitus.
(2008)
O'Neill, Heidi; Lebeck, Janne; Collins, Patrick B; Kwon, Tae-Hwan; Frøkiaer, Jørgen; Nielsen, Søren
Abstract:
Published by Oxford University Press http://ndt.oxfordjournals.org/cgi/reprint/23/5/1546
BACKGROUND: Diabetes mellitus (DM) is associated with a significant polyuria and natriuesis as well as increased plasma aldosterone and anti-diuretic hormone arginine vasopressin (AVP). This study aimed to determine whether diabetic kidneys compensate for the urinary sodium and water losses by increasing apical targeting of epithelial sodium channel (ENaC) subunits and aquaporin-2 (AQP2) in the collecting duct, in addition to the previously observed changes in ENaC subunit protein expression in different kidney zones. METHODS: Female rats were investigated 2 weeks after induction of DM by streptozotocin administration. Kidneys were examined by immunohistochemisty and semiquantitative immunoblotting. RESULTS: We demonstrated that the protein expression of renal AQP2, Ser-256 phosphorylated AQP2, AQP3, beta- and gamma-ENaC (but not alpha-ENaC) increased consistently with an increased AVP respo...
https://epubs.rcsi.ie/mctart/15
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An Assessment of the Antioxidant and Antimicrobial Activity of Six Species of Edible Irish Seaweeds
(2010)
Cox, Sabrina; Abu-Ghannam, Nissreen; Gupta, Shilpi
An Assessment of the Antioxidant and Antimicrobial Activity of Six Species of Edible Irish Seaweeds
(2010)
Cox, Sabrina; Abu-Ghannam, Nissreen; Gupta, Shilpi
Abstract:
Six species of edible Irish seaweeds; Laminaria digitata, Laminaria saccharina, Himanthalia elongata, Palmaria palmata, Chondrus crispus and Enteromorpha spirulina were screened for potential bioactivity. Extraction of secondary metabolites was carried out using different solvents to determine antioxidant and antimicrobial properties of the dried extracts. The total phenolic contents of dried methanolic extracts were significantly different (p < 0.05). H. elongata exhibited highest phenolic content at 151.3 mg GAE/g of seaweed extract and also had the highest DPPH scavenging activity (p < 0.05) with a 50% inhibition (EC50) level at 0.125μg/ml of extract. H. elongata also had the highest total tannin and total flavonoid contents (p < 0.05) of 38.34 mg CE/g and 42.5 mg QE/g, respectively. Antimicrobial activity was determined using a microtitre method which allowed detection of bacterial growth inhibition at low levels. All methanolic seaweed extracts inhibited the food spoil...
https://arrow.dit.ie/schfsehart/33
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An Evaluation of MIRU-VNTR Analysis and Spoligotyping for Genotyping of M. Bovis Isolates and a Comparison with RFLP Typing
(2009)
McLernon, Joanne; Costello, Eamon; Flynn, Orla; Madigan, Gillian; Ryan, Fergus
An Evaluation of MIRU-VNTR Analysis and Spoligotyping for Genotyping of M. Bovis Isolates and a Comparison with RFLP Typing
(2009)
McLernon, Joanne; Costello, Eamon; Flynn, Orla; Madigan, Gillian; Ryan, Fergus
Abstract:
Common strain typing methods for differentiation of Mycobacterium bovis isolates include restriction endonuclease analysis (REA), restriction fragment length polymorphism (RFLP) analysis, spoligotyping and more recently, mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing. Strain typing of Mycobacterium bovis isolates based on the variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR) and on spoligotyping was evaluated in this study and these typing methods were compared with restriction fragment length polymorphism (RFLP) typing. A total of 386 M. bovis isolates from cattle, badgers and deer in the Republic of Ireland that had previously been typed by IS6110, polymorphic GC-rich sequence (PGRS) and direct repeat (DR) RFLP were included in the study. Spoligotyping and analysis of six VNTR loci (2163a, 2163b, 2165, 4052, 2996 and 1895) was performed on the samples. RFLP was the method that gave the greatest di...
https://arrow.dit.ie/scschbioart/72
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An Example of Developing Food Based Dietary Guidelines
(2001)
Kearney, John M; Gibney, Michael
An Example of Developing Food Based Dietary Guidelines
(2001)
Kearney, John M; Gibney, Michael
https://arrow.dit.ie/scschbiocon/8
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An Investigation of the Safety of High Risk Sandwich Bar Foods Through the Application of Microbial Analysis, Temperature Monitoring and Microbial Predictive Modelling
(2002)
Maher, Marie-Clare, (Thesis)
An Investigation of the Safety of High Risk Sandwich Bar Foods Through the Application of Microbial Analysis, Temperature Monitoring and Microbial Predictive Modelling
(2002)
Maher, Marie-Clare, (Thesis)
Abstract:
Rising incidents of food poisoning may be related to growing evidence of unsatisfactory temperature and hygiene practices in preparing and storing ready-to-eat foods. Four sandwich bar outlets in Dublin city center were observed regarding the quality of food handling and storage practices of chilled vacuum packed cooked chicken breast. A pilot microbial sampling plan proved Staphylococcus aureus was the major microorganism of concern. Microbial counts revealed a S. aureus count of ) cfu/g on chicken straight form the vacuum pack and a count of 10(3)-10(4) cfu/g after handling/preparation (cutting, slicing and blending) had occurred. This compared poorly to the upper satisfactory limit of 10(2)-10(4) cfu/g. a full temperature history of the chill-display units showed fluctuations between 6-12°C, when the recommended temperature is -1 to +5°C. The potential of microbial predictive modelling was also investigated to asses the outlets degree of safety in the operating systems. Pathogen ...
https://arrow.dit.ie/tourmas/9
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Analysis of Enzyme, Carbohydrate and Mineral Distribution In the Foot of Abalone Shellfish.
(2006)
Harris, Leanne F; O'Byrne-Ring, Nuala; Lambkin, Helen
Analysis of Enzyme, Carbohydrate and Mineral Distribution In the Foot of Abalone Shellfish.
(2006)
Harris, Leanne F; O'Byrne-Ring, Nuala; Lambkin, Helen
https://arrow.dit.ie/scschbioart/156
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Analysis of Progesterone-regulated Target Genes in Mammary Gland and Uterine Development
(2009)
Walsh, Claire A.
Analysis of Progesterone-regulated Target Genes in Mammary Gland and Uterine Development
(2009)
Walsh, Claire A.
Abstract:
Progesterone is an ovarian steroid hormone which plays a very important part in the regulation of pregnancy-related changes. Progesterone functions by binding to its specific nuclear receptor, PR, which regulates defined genes in a ligand dependent manner. The aim of my thesis is to explore and elucidate some of the important mediators of progesterone action with regards to pregnancy-related mammary gland development and also uterine development. Knockout mouse models and comparative wild type mice were used in order to explore the expression patterns of specific genes of interest. Quantitative PCR using cDNA derived from mammary gland tissue was used to analyse the differences in gene expression of progesterone’s downstream target molecules. Established target genes such as RANKL, Wnt4 and Amphiregulin, TGF-β1, Sfrp2 and Mig6 were analysed to determine their mRNA expression levels in the late stages of pregnancy where growth and proliferation is at its most intense. Comparisons wer...
https://arrow.dit.ie/scienmas/48
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Application of Baranyi function to model the antibacterial properties of solvent extract from Irish York cabbage against food spoilage and pathogenic bacteria
(2011)
Jaiswal, Amit; Abu-Ghannam, Nissreen; Gupta, Shilpi; Cox, Sabrina
Application of Baranyi function to model the antibacterial properties of solvent extract from Irish York cabbage against food spoilage and pathogenic bacteria
(2011)
Jaiswal, Amit; Abu-Ghannam, Nissreen; Gupta, Shilpi; Cox, Sabrina
Abstract:
Cabbage is a rich source of a number of bioactive compounds such as flavonoids, glucosinolates and their breakdown products which may have antibacterial, antioxidant and anticancer properties. Outer green leaves of York cabbage were extracted with 60% methanol, ethanol or acetone. Antibacterial activities of vacuum dried crude extracts were estimated against a number of Gram-positive and Gram-negative food spoilage and food pathogenic bacteria. The crude extracts showed a broad spectrum of antibacterial activities but 60% methanol extract exhibited the highest antibacterial effect. Complete growth inhibition for Listeria monocytogenes was achieved with an extract concentration of 1.4%, whereas a two-fold concentration was required to achieve a reduction of 75% and 64% for Salmonella abony and Pseudomonas aeruginosa, respectively. For Enterococcus faecalis methanolic extract showed a weak inhibition only (31%). The lower concentrations of methanolic extract from York cabbage prolonge...
https://arrow.dit.ie/schfsehart/88
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Arginine-to-lysine Substitutions Influence Recombinant Horseradish Peroxidase Stability and Immobilisation Effectiveness.
(2007)
Ryan, Barry; Fagan, Ciarán
Arginine-to-lysine Substitutions Influence Recombinant Horseradish Peroxidase Stability and Immobilisation Effectiveness.
(2007)
Ryan, Barry; Fagan, Ciarán
Abstract:
Background: Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. Often, it is used in immobilised form. With conventional immobilisation techniques, the enzyme adheres in random orientation: the active site may face the solid phase rather than bulk medium, impeding substrate access and leading to sub-optimal catalytic performance. The ability to immobilise HRP in a directional manner, such that the active site would always face outwards from the insoluble matrix, would maximise the immobilised enzyme’s catalytic potential and could increase HRP’s range of actual and potential applications. Results: We have replaced arginine residues on the face of glycan-free recombinant HRP opposite to the active site by lysines. Our strategy differs from previous reports of specific HRP immobilisation via an engineered affinity tag or single reactive residue. These conservative Arg-to-Lys substitutions provide a mea...
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