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Subject = Next Generation Medical Devices;
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Displaying Results 51 - 75 of 239 on page 3 of 10
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Degradation of arterial collagen with applied strain: critical influence of matrix content and collagen crimp
(2018)
GAUL, ROBERT; NOLAN, DAVID; LALLY, CAITRIONA
Degradation of arterial collagen with applied strain: critical influence of matrix content and collagen crimp
(2018)
GAUL, ROBERT; NOLAN, DAVID; LALLY, CAITRIONA
http://hdl.handle.net/2262/85127
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Chondrogenesis and integration of mesenchymal stem cells within an in vitro cartilage defect repair model
(2009)
BUCKLEY, CONOR; KELLY, DANIEL; VINARDELL, TATIANA
Chondrogenesis and integration of mesenchymal stem cells within an in vitro cartilage defect repair model
(2009)
BUCKLEY, CONOR; KELLY, DANIEL; VINARDELL, TATIANA
Abstract:
Integration of repair tissue is a key indicator of the long-term success of cell-based therapies for cartilage repair. The objective of this study was to compare the in vitro chondrogenic differentiation and integration of agarose hydrogels seeded with either chondrocytes or bone marrow derived mesenchymal stem cells (MSCs) in defects created in cartilage explants. Chondrocytes and MSCs were isolated from porcine donors, suspended in 2% agarose and then injected into cylindrical defects within the explants. These constructs were maintained in a chemically defined medium supplemented with 10 ng/ml of TGF-?3. Cartilage integration was assessed by histology and mechanical push-out tests. After 6 weeks in culture, chondrocyte seeded constructs demonstrated a higher integration strength (64.4 ? 8.3 kPa) compared to MSC seeded constructs (22.7 ? 5.9 kPa). GAG (1.27 ? 0.3 kPa vs 0.19 ? 0.03 kPa) and collagen (0.31 ? 0.08 kPa vs 0.09 ? 0.01 kPa) accumulation in chondrocyte seeded constructs...
http://hdl.handle.net/2262/39342
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Clinical clustering of eight orthostatic haemodynamic patterns in The Irish Longitudinal Study on Ageing (TILDA)
(2020)
Romero-Ortuno, Roman; Kenny, Rose; Hern?ndez, Belinda
Clinical clustering of eight orthostatic haemodynamic patterns in The Irish Longitudinal Study on Ageing (TILDA)
(2020)
Romero-Ortuno, Roman; Kenny, Rose; Hern?ndez, Belinda
Abstract:
Background: Orthostatic hypotension (OH) can be assessed with non-invasive continuous beat-to-beat haemodynamic monitoring during active stand (AS) testing; this yields large volumes of data outside the scope of the traditional OH definition. We explored clinical associations of different AS patterns in participants from Wave 1 of The Irish Longitudinal Study on Ageing (TILDA). Methods: AS patterns were generated based on three sequential binary systolic blood pressure features: drop ?40mmHg within 10 seconds post-stand (?immediate deficit?), failure to return to within 20mmHg of supine level at 40 seconds after standing (?stabilisation deficit?), and drop ?20mmHg between >40 and 120 seconds post-stand (?late deficit?). Eight AS groups resulted from combining the presence/absence of these three features. The groups were cross-sectionally characterised, and their ability to independently predict orthostatic intolerance (OI) during AS, and falls or syncope in t...
http://hdl.handle.net/2262/92920
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Measuring Synchrony in Dialog Transcripts
(2012)
VOGEL, CARL
Measuring Synchrony in Dialog Transcripts
(2012)
VOGEL, CARL
Abstract:
A finite register method of processing dialog transcripts is used to measure interlocutor synchrony. Successive contributions by participants are measured for word n-gram repetitions and temporal overlaps. The Zipfian distribution of words in language use leads to a natural expectation that random re-orderings of dialog contributions will unavoidably exhibit repetition -- one might reasonably expect that the frequency of repetition in actual dialog is in fact best explained as a random effect. Accordingly, significance is assessed with respect to randomized contrast values. The contrasts are obtained from averages over randomized reorderings of dialog contributions with temporal spans of the revised dialogs guided by the original durations. Benchmark distributions for allo-repetition and self-repetition are established from existing dialog transcripts covering a pair of pragmatically different circumstances: ATR English language ``lingua franca'' discussions, Air-Traffic c...
http://hdl.handle.net/2262/67167
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Collagen fibre orientation and dispersion govern ultimate tensile strength, stiffness and the fatigue performance of bovine pericardium
(2019)
WHELAN, ALISON; Lally, Caitriona; Murphy, Bruce; Gaul, Robert; Nolan, David; Duffy, Joh...
Collagen fibre orientation and dispersion govern ultimate tensile strength, stiffness and the fatigue performance of bovine pericardium
(2019)
WHELAN, ALISON; Lally, Caitriona; Murphy, Bruce; Gaul, Robert; Nolan, David; Duffy, John; O'Reilly, David; Gunning, Paul
Abstract:
The durability of bovine pericardium leaflets employed in bioprosthetic heart valves (BHVs) can significantly limit the longevity of heart valve prostheses. Collagen fibres are the dominant load bearing component of bovine pericardium, however fibre architecture within leaflet geometries is not explicitly controlled in the manufacture of commercial devices. Thus, the purpose of this study was to ascertain the influence of pre-determined collagen fibre orientation and dispersion on the mechanical performance of bovine pericardium. Three tissue groups were tested in uniaxial tension: cross-fibre tissue (XD); highly dispersed fibre-orientations (HD); or preferred-fibre tissue (PD). Both the XD and PD tissue were tested under cyclic loading at 1.5?Hz and a stress range of 2.7?MPa. The results of the static tensile experiments illustrated that collagen fibre orientation and degree of alignment significantly influenced the material's response, whereby, there was a statistically sig...
http://hdl.handle.net/2262/91424
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Combined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cells.
(2014)
Ahearne, Mark; Lysaght, Joanne
Combined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cells.
(2014)
Ahearne, Mark; Lysaght, Joanne
Abstract:
BACKGROUND: Interest in adipose-derived stem cells (ASCs) has increased in recent years due to their multi-linage differentiation capabilities. While much work has been done to optimize the differentiation media, few studies have focused on examining the influence of different expansion media on cell behavior. In this study, three basal media (low glucose Dulbecco's modified Eagle's medium (DMEM), high glucose DMEM and DMEM-F12) supplemented with or without fibroblast growth factor 2 (FGF) were examined to assess their suitability for expanding ASCs. FINDINGS: Flow cytometry, colony-forming unit assays (CFU-Fs) and differentiation assays were utilized to study cell behavior. High glucose media CFU-Fs produced fewest colonies while the addition of FGF increased colony size. By passage 2, the majority of cells were positive for CD44, 45, 73, 90 and 105 and negative for CD14, 31 and 45, indicating a mesenchymal phenotype. A sub-population of CD34 positive cells was present ...
http://hdl.handle.net/2262/75539
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Combining BMP-6, TGF-β3 and hydrostatic pressure stimulation enhances the functional development of cartilage tissues engineered using human infrapatellar fat pad derived stem cells
(2013)
Kelly, Daniel; Buckley, Conor
Combining BMP-6, TGF-β3 and hydrostatic pressure stimulation enhances the functional development of cartilage tissues engineered using human infrapatellar fat pad derived stem cells
(2013)
Kelly, Daniel; Buckley, Conor
Abstract:
The objective of this study was to identify a combination of growth factors that could be used with hydrostatic pressure (HP) stimulation to enhance the functional development of cartilaginous grafts engineered using human infrapatellar fat pad derived stem cells (FPSCs) isolated from osteoarthritic patients. Agarose hydrogels were first seeded with FPSCs at different seeding densities and maintained in a chondrogenic media supplemented with TGF-?3. It was found that chondrogenesis of human FPSCs in hydrogel culture is dependent on the cell seeding density (10 versus 30 million cells per ml), with greater sulphated glycosaminoglycan (sGAG) and collagen synthesis (normalised to DNA content) observed at higher seeding densities. Additional supplementation with BMP-6 was found to augment cartilage-specific matrix synthesis, also in a cell seeding density dependent manner, increasing both cell proliferation and sGAG synthesis in constructs seeded at higher densities, but having no signi...
http://hdl.handle.net/2262/67733
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Combining freshly isolated chondroprogenitor cells from the infrapatellar fat pad with a growth factor delivery hydrogel as a putative single stage therapy for articular cartilage repair
(2014)
AHEARNE, MARK; KELLY, DANIEL
Combining freshly isolated chondroprogenitor cells from the infrapatellar fat pad with a growth factor delivery hydrogel as a putative single stage therapy for articular cartilage repair
(2014)
AHEARNE, MARK; KELLY, DANIEL
Abstract:
Growth factor delivery systems incorporating chondroprogenitor cells are an attractive potential treatment option for damaged cartilage. The rapid isolation, processing, and implantation of therapeutically relevant numbers of autologous chondroprogenitor cells, all performed "in-theatre" during a single surgical procedure, would significantly accelerate the clinical translation of such tissue engineered implants by avoiding the time, financial and regulatory challenges associated with in vitro cell expansion, and differentiation. The first objective of this study was to explore if rapid adherence to a specific substrate could be used as a simple means to quickly identify a subpopulation of chondroprogenitor cells from freshly digested infrapatellar fat pad (IFP) tissue. Adhesion of cells to tissue culture plastic within 30?min was examined as a mechanism of isolating subpopulations of cells from the freshly digested IFP. CD90, a cell surface marker associated with cell adh...
http://hdl.handle.net/2262/75544
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Comparative Imaging Study in Ultrasound, MRI, CT and DSA using a Multi-Modality Renal Artery Phantom
(2011)
FAGAN, ANDREW
Comparative Imaging Study in Ultrasound, MRI, CT and DSA using a Multi-Modality Renal Artery Phantom
(2011)
FAGAN, ANDREW
Abstract:
Purpose: A range of anatomically-realistic multi-modality renal artery phantoms consisting of vessels with varying degrees of stenosis was developed and evaluated using four imaging techniques currently used to detect renal artery stenosis (RAS). The spatial resolution required to visualize vascular geometry and the velocity detection performance required to adequately characterize blood flow in patients suffering from RAS is currently ill-defined, with the result that no one imaging modality has emerged as a gold standard technique for screening for this disease. Methods: The phantoms, which contained a range of stenosis values (0, 30, 50, 70 and 85%), were designed for use with ultrasound, magnetic resonance imaging, X ray computed tomography and X-ray digital subtraction angiography. The construction materials used were optimized with respect to their ultrasonic speed of sound and attenuation coefficient, MR relaxometry (T1, T2) properties, and Hounsfield number / X-ray attenu...
http://hdl.handle.net/2262/56864
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Comparing SpamAssassin with CBDF email filtering
(2004)
VOGEL, CARL
Comparing SpamAssassin with CBDF email filtering
(2004)
VOGEL, CARL
Abstract:
In this paper, we compare the email filtering software SpamAssassin with a statistical email filter, known as chi by degrees of freedom. We examine SpamAssassin?s filtering techniques and ascertain their effectiveness. By comparing the two filtering methods, we will show that a rule based filter such as SpamAssassin is in fact the wrong approach for something as inconstant as unsolicited bulk email.
http://hdl.handle.net/2262/32956
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Comparing the Effects of Intracellular and Extracellular Magnetic Hyperthermia on the Viability of BxPC-3 Cells
(2020)
HANNON, GARY; Bogdanska, Anna; Volkov, Yuri; Prina-Mello, Adriele
Comparing the Effects of Intracellular and Extracellular Magnetic Hyperthermia on the Viability of BxPC-3 Cells
(2020)
HANNON, GARY; Bogdanska, Anna; Volkov, Yuri; Prina-Mello, Adriele
Abstract:
Magnetic hyperthermia involves the use of iron oxide nanoparticles to generate heat in tumours following stimulation with alternating magnetic fields. In recent times, this treatment has undergone numerous clinical trials in various solid malignancies and subsequently achieved clinical approval to treat glioblastoma and prostate cancer in 2011 and 2018, respectively. However, despite recent clinical advances, many questions remain with regard to the underlying mechanisms involved in this therapy. One such query is whether intracellular or extracellular nanoparticles are necessary for treatment efficacy. Herein, we compare the effects of intracellular and extracellular magnetic hyperthermia in BxPC-3 cells to determine the differences in efficacy between both. Extracellular magnetic hyperthermia at temperatures between 40?42.5 ?C could induce significant levels of necrosis in these cells, whereas intracellular magnetic hyperthermia resulted in no change in viability. This led to a di...
http://hdl.handle.net/2262/92368
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Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad
(2011)
KELLY, DANIEL; BUCKLEY, CONOR; VINARDELL, TATIANA
Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad
(2011)
KELLY, DANIEL; BUCKLEY, CONOR; VINARDELL, TATIANA
Abstract:
The objective of this study was to determine the functional properties of cartilaginous tissues generated by porcine MSCs isolated from different tissue sources, and to compare these properties to those derived from chondrocytes (CC). MSCs were isolated from bone marrow (BM) and infrapatellar fat pad (FP), while CC were harvested from the articular surface of the femoro-patellar joint. Culture-expanded CC and MSCs were encapsulated in agarose hydrogels and cultured in the presence of TGF-?3. Samples were analysed biomechanically, biochemically and histologically at day 0, day 21 and day 42. After 42 days in free swelling culture, mean GAG content was 1.50 % w/w in CC seeded constructs, compared to 0.95 % w/w in FP and 0.43 % w/w in BM seeded constructs. Total collagen accumulation was highest in FP constructs. DNA content increased with time for all the groups. The mechanical functionality of cartilaginous tissues engineered using CCs was superior to that generated from either sourc...
http://hdl.handle.net/2262/60816
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Computational simulation methodologies for mechanobiological modelling: a cell-centred approach to neointima development in stents.
(2010)
PRENDERGAST, PATRICK; LENNON, ALEXANDER; KELLY, DANIEL; BOYLE, COLIN; EARLY, MICHAEL; L...
Computational simulation methodologies for mechanobiological modelling: a cell-centred approach to neointima development in stents.
(2010)
PRENDERGAST, PATRICK; LENNON, ALEXANDER; KELLY, DANIEL; BOYLE, COLIN; EARLY, MICHAEL; LALLY, CAITRIONA
Abstract:
The design of medical devices could be very much improved if robust tools were available for computational simulation of tissue response to the presence of the implant. Such tools require algorithms to simulate the response of tissues to mechanical and chemical stimuli. Available methodologies include those based on the principle of mechanical homeostasis, those which use continuum models to simulate biological constituents, and the cell-centred approach, which models cells as autonomous agents. In the latter approach, cell behaviour is governed by rules based on the state of the local environment around the cell; and informed by experiment. Tissue growth and differentiation requires simulating many of these cells together. In this paper, the methodology and applications of cell-centred techniques?with particular application to mechanobiology?are reviewed, and a cell-centred model of tissue formation in the lumen of an artery in response to the deployment of a stent is presented. Th...
http://hdl.handle.net/2262/39846
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Computer simulating a clinical trial of a load-bearing implant: example of an intramedullary prosthesis
(2011)
PRENDERGAST, PATRICK
Computer simulating a clinical trial of a load-bearing implant: example of an intramedullary prosthesis
(2011)
PRENDERGAST, PATRICK
Abstract:
Computational modelling is becoming ever more important for obtaining regulatory approval for new medical devices. An accepted approach is to infer performance in a population from an analysis conducted in an idealized or `average? patient; we present here a method for predicting the performance of an orthopaedic implant when released into a population?effectively simulating a clinical trial. Specifically we hypothesise an analysis based on a method for predicting the performance in a population will lead to different conclusions than an analysis based on an idealised or `average? patient. To test this hypothesis we use a finite element model of an intramedullary implant in a bone whose size and remodelling activity is different for each individual in the population. We compare the performance of a low Young?s modulus implant () to one with a higher Young?s modulus (200 GPa). Cyclic loading is applied and failure is assumed when the migration of the implant relative to the bone exce...
http://hdl.handle.net/2262/61633
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Conformational Re-engineering of Porphyrins as Receptors with Switchable NH???X-Type Binding Modes
(2019)
Senge, Mathias; Norvai?a, Karolis; Flanagan, Keith J.; Gibbons, D?ire
Conformational Re-engineering of Porphyrins as Receptors with Switchable NH???X-Type Binding Modes
(2019)
Senge, Mathias; Norvai?a, Karolis; Flanagan, Keith J.; Gibbons, D?ire
Abstract:
The selectivity and functional variability of porphyrin cofactors are typically based on substrate binding of metalloporphyrins wherein the pyrrole nitrogen units only serve to chelate the metal ions. Yet, using the porphyrin inner core system for other functions is possible through conformational engineering. As a first step towards porphyrin ?enzyme?like? active centers, a structural and spectroscopic study of substrate binding to the inner core porphyrin system shows that a highly saddle?distorted porphyrin with peripheral amino receptor groups (1, 2,3,7,8,12,13,17,18?octaethyl?5,10,15,20?tetrakis(2?aminophenyl)porphyrin) coordinates analytes in a switchable manner dependent on the acidity of the solution. The supramolecular ensemble exhibits exceptionally high affinity to and selectivity for the pyrophosphate anion (2.26?0.021)?109?m?1. 1H?NMR spectroscopic studies provided insight into the likely mode of binding and the characterization of atropisomers, all four of which were a...
http://hdl.handle.net/2262/91291
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Controlled release of transforming growth factor-?3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells.
(2014)
KELLY, DANIEL; O'BRIEN, FERGAL; CUNNIFFE, GR?INNE
Controlled release of transforming growth factor-?3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells.
(2014)
KELLY, DANIEL; O'BRIEN, FERGAL; CUNNIFFE, GR?INNE
Abstract:
Abstract: The objective of this study was to develop a scaffold derived from cartilaginous extracellular matrix (ECM) that could be used as a growth factor delivery system to promote chondrogenesis of stem cells. Dehyd rothermal crosslinked scaffolds were fabricated using a slurry of homogenized porcine articular cartilage, which were then seeded with human infrapatellar fat pad derived stem cells (FPSCs). It was found that these ECM derived scaffolds promoted superior c hondrogenesis of FPSCs when the constructs were additionally stimulated with transforming growth factor (TGF) - ?3. Cell mediated contraction of the scaffold was observed, which could be limited by the additional use of 1 - Ethyl - 3 - 3dimethyl aminopropyl carbod iimide (EDAC) crosslinking without suppressing cartilage specific matrix accumulation within the construct. To further validate the utility of the ECM derived scaffold, we next compared its chondro - permissive properties to a biomimetic collagen - hyaluro...
http://hdl.handle.net/2262/72871
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Coupling freshly isolated CD44+ infrapatellar fat pad derived stromal cells with a tgf-β3 eluting cartilage ecm-derived scaffold as a single stage strategy for promoting chondrogenesis.
(2015)
KELLY, DANIEL; VINARDELL, TATIANA; BUCKLEY, CONOR
Coupling freshly isolated CD44+ infrapatellar fat pad derived stromal cells with a tgf-β3 eluting cartilage ecm-derived scaffold as a single stage strategy for promoting chondrogenesis.
(2015)
KELLY, DANIEL; VINARDELL, TATIANA; BUCKLEY, CONOR
http://hdl.handle.net/2262/82240
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Crosslinking and mechanical properties significantly influence cell attachment, proliferation, and migration within collagen glycosaminoglycan scaffolds.
(2011)
O'BRIEN, FERGAL
Crosslinking and mechanical properties significantly influence cell attachment, proliferation, and migration within collagen glycosaminoglycan scaffolds.
(2011)
O'BRIEN, FERGAL
Abstract:
Crosslinking and the resultant changes in mechanical properties have been shown to influence cellular activity within collagen biomaterials. With this in mind, we sought to determine the effects of crosslinking on both the compressive modulus of collagen-glycosaminoglycan scaffolds and the activity of osteoblasts seeded within them. Dehydrothermal, 1-ethyl-3-3-dimethyl aminopropyl carbodiimide and glutaraldehyde crosslinking treatments were first investigated for their effect on the compressive modulus of the scaffolds. After this, the most promising treatments were used to study the effects of crosslinking on cellular attachment, proliferation, and infiltration. Our experiments have demonstrated that a wide range of scaffold compressive moduli can be attained by varying the parameters of the crosslinking treatments. 1-Ethyl-3-3-dimethyl aminopropyl carbodiimide and glutaraldehyde treatments produced the stiffest scaffolds (fourfold increase when compared to dehydrothermal crosslink...
http://hdl.handle.net/2262/55329
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Cyclic Hydrostatic Pressure Promotes A Stable Cartilage Phenotype And Enhances The Functional Development Of Cartilaginous Grafts Engineered Using Multipotent Stromal Cells Isolated From Bone Marrow And Infrapatellar Fat Pad.
(2014)
BUCKLEY, CONOR; KELLY, DANIEL
Cyclic Hydrostatic Pressure Promotes A Stable Cartilage Phenotype And Enhances The Functional Development Of Cartilaginous Grafts Engineered Using Multipotent Stromal Cells Isolated From Bone Marrow And Infrapatellar Fat Pad.
(2014)
BUCKLEY, CONOR; KELLY, DANIEL
Abstract:
The objective of this study was to investigate how joint specific biomechanical loading influences the functional development and phenotypic stability of cartilage grafts engineered in vitro using stem/progenitor cells isolated from different source tissues. Porcine bone marrow derived multipotent stromal cells (BMSCs) and infrapatellar fat pad derived multipotent stromal cells (FPSCs) were seeded in agarose hydrogels and cultured in chondrogenic medium, while simultaneously subjected to 10 MPa of cyclic hydrostatic pressure (HP). To mimic the endochondral phenotype observed in vivo with cartilaginous tissues engineered using BMSCs, the culture media was additionally supplemented with hypertrophic factors, while the loss of phenotype observed in vivo with FPSCs was induced by withdrawing transforming growth factor (TGF)-?3 from the media. The application of HP was found to enhance the functional development of cartilaginous tissues engineered using both BMSCs and FPSCs. In addition,...
http://hdl.handle.net/2262/68228
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Decellularized grafts with axially aligned channels for peripheral nerve regeneration.
(2015)
Reilly, Richard; Buckley, Conor
Decellularized grafts with axially aligned channels for peripheral nerve regeneration.
(2015)
Reilly, Richard; Buckley, Conor
http://hdl.handle.net/2262/80185
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Decoding of attentional selection in a cocktail party environment from single-trial EEG is robust to task.
(2014)
REILLY, RICHARD; LALOR, EDMUND
Decoding of attentional selection in a cocktail party environment from single-trial EEG is robust to task.
(2014)
REILLY, RICHARD; LALOR, EDMUND
http://hdl.handle.net/2262/75638
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Defining the impaction frequency and threshold force required for femoral impaction grafting in revision hip arthroplasty. A human cadaveric mechanical study.
(2011)
O'REILLY, PETER; KELLY, DANIEL
Defining the impaction frequency and threshold force required for femoral impaction grafting in revision hip arthroplasty. A human cadaveric mechanical study.
(2011)
O'REILLY, PETER; KELLY, DANIEL
Abstract:
BACKGROUND AND PURPOSE: The two most common complications of femoral impaction bone grafting are femoral fracture and massive implant subsidence. We investigated fracture forces and implant subsidence rates in embalmed human femurs undergoing impaction grafting. The study consisted of two arms, the first examining the force at which femoral fracture occurs in the embalmed human femur, and the second examining whether significant graft implant/subsidence occurs following impaction at a set force at two different impaction frequencies. METHODS: Using a standardized impaction grafting technique with modifications, an initial group of 17 femurs underwent complete destructive impaction testing, allowing sequentially increased, controlled impaction forces to be applied until femoral fracture occurred. A second group of 8 femurs underwent impaction bone grafting at constant force, at an impaction frequency of 1 Hz or 10 Hz. An Exeter stem was cemented into the neomedullary canals. These co...
http://hdl.handle.net/2262/60181
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Determination of spiropyran cytotoxicity by high content screening and analysis for safe application in bionanosensing
(2010)
GIORDANI, SILVIA; MOVIA, DANIA; VOLKOV, YURI; PRINA MELLO, ADRIELE
Determination of spiropyran cytotoxicity by high content screening and analysis for safe application in bionanosensing
(2010)
GIORDANI, SILVIA; MOVIA, DANIA; VOLKOV, YURI; PRINA MELLO, ADRIELE
Abstract:
The in vitro toxic response of spiropyrans in cellular models has not been previously addressed, despite the fact that such photoswitchable molecules have shown great potential as versatile and tunable components for bionanodevices and imaging agents. In this study, we examine the cytotoxic effects of a spiropyran, namely, 8-methoxy-6-nitro-BIPS (1?,3?-dihydro-1?-ethanol-3?,3?-dimethyl-8-methoxy-6-nitro-spiro(2H-1-benzopyran-2,2?-(2H)-indole) [1], in three cultured cellular models (THP-1, AGS, and A549 cell lines) by High Content Screening and Analysis (HCSA) and by enzyme-linked immunosorbent (ELISA) assays (Interleukin-6 and Tumor Necrosis Factor-alpha). The HCSA results show that low concentrations of 8-methoxy-6-nitro-BIPS (10?6, 10?8, and 10?9 M) do not induce any cytotoxic response after 24 and 72 h exposure time, while at the highest concentrations (10?3and 10?4 M) the exposure time becomes a critical parameter of the toxic response. The cell viability is reduced by 60% for T...
http://hdl.handle.net/2262/93088
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Dextran Preserves Native Corneal Structure During Decellularization.
(2016)
Ahearne, Mark
Dextran Preserves Native Corneal Structure During Decellularization.
(2016)
Ahearne, Mark
Abstract:
Corneal decellularization has become an increasingly popular technique for generating scaffolds for corneal regeneration. Most decellularization procedures result in tissue swelling, thus limiting their application. Here, the use of a polysaccharide, dextran, to reduce swelling and conserve the native corneal structure during decellularization was investigated. Corneas were treated with 1% Triton X-100, 0.5% sodium dodecyl sulfate, and nucleases under constant rotation followed by extensive washing. To reduce swelling, decellularization solutions were supplemented with 5% dextran either throughout the whole decellularization process or during the washing cycles only. Quantitative analysis of DNA content showed a 96% reduction after decellularization regardless of the addition of dextran. Dextran resulted in a significant reduction in swelling from 3.85 ? 0.43 nm without to 1.94 ? 0.29-2.01 ? 0.37 nm (p < 0.05) remaining at similar dimensions to the native tissue (1.73 ? 0.23 nm)....
http://hdl.handle.net/2262/95727
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Differential response of encapsulated nucleus pulposus and bone marrow stem cells in isolation and coculture in alginate and chitosan hydrogels
(2015)
Buckley, Conor
Differential response of encapsulated nucleus pulposus and bone marrow stem cells in isolation and coculture in alginate and chitosan hydrogels
(2015)
Buckley, Conor
http://hdl.handle.net/2262/80190
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