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Limited By:	Subject = Reducing agent;

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Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle (2018)

Adnane, Mounir; Kelly, Paul M; Chapwanya, Aspinas; Meade, Kieran G; O'Farrelly, Cl...

Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle (2018)

Adnane, Mounir; Kelly, Paul M; Chapwanya, Aspinas; Meade, Kieran G; O'Farrelly, Cliona

Abstract:

Background In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved method for processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and α1-acid glycoprotein (AGP) were measured by ELISA. Results We found that use of reducing agents to ...

http://hdl.handle.net/11019/1603

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Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle (2018)

O'Farrelly, Cliona; Adnane, Mounir; Kelly, Paul; Chapwanya, Aspinas; Meade, Kieran

Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle (2018)

O'Farrelly, Cliona; Adnane, Mounir; Kelly, Paul; Chapwanya, Aspinas; Meade, Kieran

Abstract:

Background: In the postpartum cow, early diagnosis of uterine disease is currently problematic due to the lack of reliable, non-invasive diagnostic methods. Cervico-vaginal mucus (CVM) is an easy to collect potentially informative source of biomarkers for the diagnosis and prognosis of uterine disease in cows. Here, we report an improved methodfor processing CVM from postpartum dairy cows for the measurement of immune biomarkers. CVM samples were collected from the vagina using gloved hand during the first two weeks postpartum and processed with buffer alone or buffer containing different concentrations of the reducing agents recommended in standard protocols: Dithiothriotol (DTT) or N-Acetyl-L-Cysteine (NAC). Total protein was measured using the bicinchoninic acid (BCA) assay; interleukin 6 (IL-6), IL-8 and?1-acid glycoprotein (AGP) were measured by ELISA. Results: We found that use of reducing agents to liquefy CVM affects protein yield and the accuracy of biomarker detection. Our...

http://hdl.handle.net/2262/89581

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Kinetics and coverage dependent reaction mechanisms of the copper atomic layer deposition from copper dimethylamino-2-propoxide and diethylzinc (2016)

Maimaiti, Yasheng; Elliott, Simon D.

Kinetics and coverage dependent reaction mechanisms of the copper atomic layer deposition from copper dimethylamino-2-propoxide and diethylzinc (2016)

Maimaiti, Yasheng; Elliott, Simon D.

Abstract:

Atomic layer deposition (ALD) has been recognized as a promising method to deposit conformal and uniform thin film of copper for future electronic devices. However, many aspects of the reaction mechanism and the surface chemistry of copper ALD remain unclear. In this paper, we employ plane wave density functional theory (DFT) to study the transmetalation ALD reaction of copper dimethylamino-2-propoxide [Cu(dmap)2] and diethylzinc [Et2Zn] that was realized experimentally by Lee et al. [ Angew. Chem., Int. Ed. 2009, 48, 4536−4539]. We find that the Cu(dmap)2 molecule adsorbs and dissociates through the scission of one or two Cu–O bonds into surface-bound dmap and Cu(dmap) fragments during the copper pulse. As Et2Zn adsorbs on the surface covered with Cu(dmap) and dmap fragments, butane formation and desorption was found to be facilitated by the surrounding ligands, which leads to one reaction mechanism, while the migration of ethyl groups to the surface leads to another reaction mecha...

http://hdl.handle.net/10468/3236

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Mechanism for the atomic layer deposition of copper using diethylzinc as the reducing agent – a density functional theory study using gas phase molecules as a model (2012)

Dey, Gangotri; Elliott, Simon D.

Mechanism for the atomic layer deposition of copper using diethylzinc as the reducing agent – a density functional theory study using gas phase molecules as a model (2012)

Dey, Gangotri; Elliott, Simon D.

Abstract:

We present theoretical studies based on first-principles density functional theory calculations for the possible gas-phase mechanism of the atomic layer deposition (ALD) of copper by transmetalation from common precursors such as Cu(acac)(2), Cu(hfac)(2), Cu(PyrIm(R))(2) with R = (i)Pr and Et, Cu(dmap)(2), and CuCl(2) where diethylzinc acts as the reducing agent. An effect on the geometry and reactivity of the precursors due to differences in electronegativity, steric hindrance, and conjugation present in the ligands was observed. Three reaction types, namely, disproportionation, ligand exchange, and reductive elimination, were considered that together comprise the mechanism for the formation of copper in its metallic state starting from the precursors. A parallel pathway for the formation of zinc in its metallic form was also considered. The model Cu(I) molecule Cu(2)L(2) was studied, as Cu(I) intermediates at the surface play an important role in copper deposition. Through our stu...

http://hdl.handle.net/10468/1054

Displaying Results 1 - 4 of 4 on page 1 of 1