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Displaying Results 1 - 22 of 22 on page 1 of 1
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A longitudinal study of amoebic gill disease on a marine atlantic salmon farm utilising a real-time pcr assay for the detection of neoparamoeba perurans
(2018)
Downes, JK; Henshilwood, K; Collins, EM; Ryan, A; O’Connor, I; Rodger, HD; MacCarthy, E...
A longitudinal study of amoebic gill disease on a marine atlantic salmon farm utilising a real-time pcr assay for the detection of neoparamoeba perurans
(2018)
Downes, JK; Henshilwood, K; Collins, EM; Ryan, A; O’Connor, I; Rodger, HD; MacCarthy, E; Ruane, NM
Abstract:
Amoebic gill disease (AGD) is a proliferative gill disease of marine cultured Atlantic salmon Salmo salar, with the free-living protozoan Neoparamoeba perurans being the primary aetiological agent. The increased incidence of AGD in recent years presents a significant challenge to the Atlantic salmon farming industry in Europe. In this study, a real-time TaqMan (R) PCR assay was developed and validated to detect Neoparamoeba perurans on Atlantic salmon gills and further used to monitor disease progression on a marine Atlantic salmon farm in Ireland in conjunction with gross gill pathology and histopathology. The assay proved specific for N. perurans, with no cross-reactivity with the related species N. pemaquidensis, N. branchiphila or N. aestuarina, and was capable of detecting 2.68 copies of N. perurans DNA mu l(-1). Although the parasite was detected throughout the 18 mo period of this study, mortality peaks associated with clinical AGD were only recorded during the first 12 mo of...
http://hdl.handle.net/10379/11247
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A novel hybrid cfh/cfhr3 gene generated by a microhomology-mediated deletion in familial atypical hemolytic uremic syndrome
(2018)
Francis, N. J.; McNicholas, B.; Awan, A.; Waldron, M.; Reddan, D.; Sadlier, D.; Kavanag...
A novel hybrid cfh/cfhr3 gene generated by a microhomology-mediated deletion in familial atypical hemolytic uremic syndrome
(2018)
Francis, N. J.; McNicholas, B.; Awan, A.; Waldron, M.; Reddan, D.; Sadlier, D.; Kavanagh, D.; Strain, L.; Marchbank, K. J.; Harris, C. L.; Goodship, T. H. J.
Abstract:
Genomic disorders affecting the genes encoding factor H (fH) and the 5 factor H related proteins have been described in association with atypical hemolytic uremic syndrome. These include deletions of CFHR3, CFHR1, and CFHR4 in association with fH autoantibodies and the formation of a hybrid CFH/CFHR1 gene. These occur through nonallelic homologous recombination secondary to the presence of large segmental duplications (macrohomology) in this region. Using multiplex ligation-dependent probe amplification to screen for such genomic disorders, we have identified a large atypical hemolytic uremic syndrome family where a deletion has occurred through microhomology mediated end joining rather than nonallelic homologous recombination. In the 3 affected persons of this family, we have shown that the deletion results in formation of a CFH/CFHR3 gene. We have shown that the protein product of this is a 24 SCR protein that is secreted with nor-mal fluid-phase activity but marked loss of comple...
http://hdl.handle.net/10379/11547
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Assessing the zoantharian diversity of the tropical eastern pacific through an integrative approach
(2018)
Jaramillo, Karla B.; Reverter, Miriam; Guillen, Paul O.; McCormack, Grace; Rodriguez, J...
Assessing the zoantharian diversity of the tropical eastern pacific through an integrative approach
(2018)
Jaramillo, Karla B.; Reverter, Miriam; Guillen, Paul O.; McCormack, Grace; Rodriguez, Jenny; Sinniger, Frédéric; Thomas, Olivier P.
Abstract:
Zoantharians represent a group of marine invertebrates widely distributed from shallow waters to the deep sea. Despite a high diversity and abundance in the rocky reefs of the Pacific Ocean, very few studies have been reported on the diversity of this group in the Tropical Eastern Pacific coasts. While molecular techniques recently clarified some taxonomic relationships within the order, the taxonomy of zoantharians is still highly challenging due to a lack of clear morphological characters and confusing use of different data in previous studies. Our first insight into the zoantharian diversity at El Pelado Marine Protected Area - Ecuador led to the identification of six species: Terrazoanthus patagonichus; Terrazoanthus sp.; Antipathozoanthus hickmani; Parazoanthus darwini; Zoanthus cf. pulchellus; and Zoanthus cf. sociatus. A metabolomic approach using UHPLC-HRMS was proven to be very efficient as a complementary tool in the systematics of these species and specialized metabolites...
http://hdl.handle.net/10379/12075
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Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation in E. coli
(2017)
Liang, Mingzhi; Frank, Stefanie; Lünsdorf, Heinrich; Warren, Martin J.; Prentice, Micha...
Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation in E. coli
(2017)
Liang, Mingzhi; Frank, Stefanie; Lünsdorf, Heinrich; Warren, Martin J.; Prentice, Michael B.
Abstract:
Processes for the biological removal of phosphate from wastewater rely on temporary manipulation of bacterial polyphosphate levels by phased environmental stimuli. In E. coli polyphosphate levels are controlled via the polyphosphate-synthesizing enzyme polyphosphate kinase (PPK1) and exopolyphosphatases (PPX and GPPA), and are temporarily enhanced by PPK1 overexpression and reduced by PPX overexpression. We hypothesised that partitioning PPK1 from cytoplasmic exopolyphosphatases would increase and stabilise E. coli polyphosphate levels. Partitioning was achieved by co-expression of E. coli PPK1 fused with a microcompartment-targeting sequence and an artificial operon of Citrobacter freundii bacterial microcompartment genes. Encapsulation of targeted PPK1 resulted in persistent phosphate uptake and stably increased cellular polyphosphate levels throughout cell growth and into the stationary phase, while PPK1 overexpression alone produced temporary polyphosphate increase and phosphate...
http://hdl.handle.net/10468/4211
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Biodistribution and retention of locally administered human mesenchymal stromal cells: Quantitative polymerase chain reaction–based detection of human DNA in murine organs
(2019)
Creane, Michael; Howard, Linda; O'Brien, Timothy; Coleman, Cynthia M.
Biodistribution and retention of locally administered human mesenchymal stromal cells: Quantitative polymerase chain reaction–based detection of human DNA in murine organs
(2019)
Creane, Michael; Howard, Linda; O'Brien, Timothy; Coleman, Cynthia M.
Abstract:
Background. Determining the distributive fate and retention of a cell therapy product after administration is an essential part of characterizing it's biosafety profile. Therefore, regulatory guidelines stipulate that biodistribution assays are a requirement prior to advancing a cell therapy to the clinic. Here the development of a highly sensitive quantitative polymerase chain reaction (qPCR)-based method of tracking the biodistribution and retention of human mesenchymal stromal cells (hMSCs) in mice, rats or rabbits is described. Methods. A primer-probe based qPCR assay was developed to detect and quantify human Alu sequences in a heterogeneous sample of human DNA (hDNA) and murine DNA from whole organ genomic DNA extracts. The assay measures the amount of genomic hDNA by amplifying a 31 base pair sequence of the human Alu (hAlu) repeat sequence, thus enabling the detection of 0.1 human cells in 1.5 x 10(6) heterogeneous cells. Results. Using this assay we investigated the bi...
http://hdl.handle.net/10379/15327
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Biohackathon series in 2011 and 2012: penetration of ontology and linked data in life science domains
(2018)
Katayama, Toshiaki; Wilkinson, Mark D; Aoki-Kinoshita, Kiyoko F; Kawashima, Shuichi; Ya...
Biohackathon series in 2011 and 2012: penetration of ontology and linked data in life science domains
(2018)
Katayama, Toshiaki; Wilkinson, Mark D; Aoki-Kinoshita, Kiyoko F; Kawashima, Shuichi; Yamamoto, Yasunori; Yamaguchi, Atsuko; Okamoto, Shinobu; Kawano, Shin; Kim, Jin-Dong; Wang, Yue; Wu, Hongyan; Kano, Yoshinobu; Ono, Hiromasa; Bono, Hidemasa; Kocbek, Simon; Aerts, Jan; Akune, Yukie; Antezana, Erick; Arakawa, Kazuharu; Aranda, Bruno
Abstract:
The application of semantic technologies to the integration of biological data and the interoperability of bioinformatics analysis and visualization tools has been the common theme of a series of annual BioHackathons hosted in Japan for the past five years. Here we provide a review of the activities and outcomes from the BioHackathons held in 2011 in Kyoto and 2012 in Toyama. In order to efficiently implement semantic technologies in the life sciences, participants formed various sub-groups and worked on the following topics: Resource Description Framework (RDF) models for specific domains, text mining of the literature, ontology development, essential metadata for biological databases, platforms to enable efficient Semantic Web technology development and interoperability, and the development of applications for Semantic Web data. In this review, we briefly introduce the themes covered by these sub-groups. The observations made, conclusions drawn, and software development projects t...
http://hdl.handle.net/10379/12159
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Bioreactor scalability: laboratory-scale bioreactor design influences performance, ecology, and community physiology in expanded granular sludge bed bioreactors
(2017)
Connelly, Stephanie; Shin, Seung Gu; Dillon, Robert J.; Ijaz, Umer Zeeshan; Quince, Chr...
Bioreactor scalability: laboratory-scale bioreactor design influences performance, ecology, and community physiology in expanded granular sludge bed bioreactors
(2017)
Connelly, Stephanie; Shin, Seung Gu; Dillon, Robert J.; Ijaz, Umer Zeeshan; Quince, Christopher; Sloan, William; Collins, Gavin
Abstract:
Studies investigating the feasibility of new, or improved, biotechnologies, such as wastewater treatment digesters, inevitably start with laboratory-scale trials. However, it is rarely determined whether laboratory-scale results reflect full-scale performance or microbial ecology. The Expanded Granular Sludge Bed (EGSB) bioreactor, which is a high-rate anaerobic digester configuration, was used as a model to address that knowledge gap in this study. Two laboratory-scale idealizations of the EGSB-a one-dimensional and a three-dimensional scale-down of a full-scale design-were built and operated in triplicate under near-identical conditions to a full-scale EGSB. The laboratory-scale bioreactors were seeded using biomass obtained from the full-scale bioreactor, and, spent water from the distillation of whisky from maize was applied as substrate at both scales. Over 70 days, bioreactor performance, microbial ecology, and microbial community physiology were monitored at various depths in...
http://hdl.handle.net/10379/6827
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Bioreactor scalability: laboratory-scale bioreactor design influences performance, ecology, and community physiology in expanded granular sludge bed bioreactors
(2018)
Connelly, Stephanie; Shin, Seung G.; Dillon, Robert J.; Ijaz, Umer Z.; Quince, Christop...
Bioreactor scalability: laboratory-scale bioreactor design influences performance, ecology, and community physiology in expanded granular sludge bed bioreactors
(2018)
Connelly, Stephanie; Shin, Seung G.; Dillon, Robert J.; Ijaz, Umer Z.; Quince, Christopher; Sloan, William T.; Collins, Gavin
Abstract:
Studies investigating the feasibility of new, or improved, biotechnologies, such as wastewater treatment digesters, inevitably start with laboratory-scale trials. However, it is rarely determined whether laboratory-scale results reflect full-scale performance or microbial ecology. The Expanded Granular Sludge Bed (EGSB) bioreactor, which is a high-rate anaerobic digester configuration, was used as a model to address that knowledge gap in this study. Two laboratory-scale idealizations of the EGSB-a one-dimensional and a three-dimensional scale-down of a full-scale design-were built and operated in triplicate under near-identical conditions to a full-scale EGSB. The laboratory-scale bioreactors were seeded using biomass obtained from the full-scale bioreactor, and, spent water from the distillation of whisky from maize was applied as substrate at both scales. Over 70 days, bioreactor performance, microbial ecology, and microbial community physiology were monitored at various depths in...
http://hdl.handle.net/10379/10873
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Comparison of approaches for rational siRNA design leading to a new efficient and transparent method
(2007)
Matveeva, Olga; Nechipurenko, Yury; Rossi, Leo; Moore, Barry; Saetrom, Pal; Ogurtsov, A...
Comparison of approaches for rational siRNA design leading to a new efficient and transparent method
(2007)
Matveeva, Olga; Nechipurenko, Yury; Rossi, Leo; Moore, Barry; Saetrom, Pal; Ogurtsov, Aleksey Y.; Atkins, John F.; Shabalina, Svetlana A.
Abstract:
Current literature describes several methods for the design of efficient siRNAs with 19 perfectly matched base pairs and 2 nt overhangs. Using four independent databases totaling 3336 experimentally verified siRNAs, we compared how well several of these methods predict siRNA cleavage efficiency. According to receiver operating characteristics (ROC) and correlation analyses, the best programs were BioPredsi, ThermoComposition and DSIR. We also studied individual parameters that significantly and consistently correlated with siRNA efficacy in different databases. As a result of this work we developed a new method which utilizes linear regression fitting with local duplex stability, nucleotide position-dependent preferences and total G/C content of siRNA duplexes as input parameters. The new method's discrimination ability of efficient and inefficient siRNAs is comparable with that of the best methods identified, but its parameters are more obviously related to the mechanisms of s...
http://hdl.handle.net/10468/5032
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Computational approach for calculating the probability of eukaryotic translation initiation from ribo-seq data that takes into account leaky scanning
(2014)
Michel, Audrey M.; Andreev, Dmitry E.; Baranov, Pavel V.
Computational approach for calculating the probability of eukaryotic translation initiation from ribo-seq data that takes into account leaky scanning
(2014)
Michel, Audrey M.; Andreev, Dmitry E.; Baranov, Pavel V.
Abstract:
BACKGROUND: Ribosome profiling (ribo-seq) provides experimental data on the density of elongating or initiating ribosomes at the whole transcriptome level that can be potentially used for estimating absolute levels of translation initiation at individual Translation Initiation Sites (TISs). These absolute levels depend on the mutual organisation of TISs within individual mRNAs. For example, according to the leaky scanning model of translation initiation in eukaryotes, a strong TIS downstream of another strong TIS is unlikely to be productive, since only a few scanning ribosomes would be able to reach the downstream TIS. In order to understand the dependence of translation initiation efficiency on the surrounding nucleotide context, it is important to estimate the strength of TISs independently of their mutual organisation, i.e. to estimate with what probability a ribosome would initiate at a particular TIS. RESULTS: We designed a simple computational approach for estimating the prob...
http://hdl.handle.net/10468/2200
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Differences in EPG contact dynamics between voiced and voiceless lingual fricatives
(2013)
Liker, Marko; Gibbon, Fiona E.
Differences in EPG contact dynamics between voiced and voiceless lingual fricatives
(2013)
Liker, Marko; Gibbon, Fiona E.
Abstract:
Achieving voicing during fricatives is complex because voicing and frication require opposite production strategies that must be managed effectively at the supralaryngeal level. Previous research has suggested that there are differences in tongue-to-palate contact patterns that are conditioned by voicing. However, findings have been restricted to a single time point and have been generally inconclusive. This study used electropalatography (EPG) to investigate differences in the dynamics of contact in voiced and voiceless lingual fricatives. Participants were six typically speaking Croatian adults. The speech material consisted of symmetrical VCV sequences, where C was /s z ʃ ʒ/. EPG measures were taken throughout the fricatives and indices were used to quantify place of articulation (CoG), groove width and target configuration onset. The EPG measures showed similar results for voiced and voiceless fricatives during their central portions. However, there were notable differences at t...
http://hdl.handle.net/10468/5703
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Gut microbiota composition correlates with diet and health in the elderly
(2012)
Claesson, Marcus J.; Jeffery, Ian B.; Conde, Susana; Power, Susan E.; O'Connor, Ei...
Gut microbiota composition correlates with diet and health in the elderly
(2012)
Claesson, Marcus J.; Jeffery, Ian B.; Conde, Susana; Power, Susan E.; O'Connor, Eibhlís M.; Cusack, Siobhán; Harris, Hugh M. B.; Coakley, Mairead; Lakshminarayanan, Bhuvaneswari; O'Sullivan, Orla; Fitzgerald, Gerald F.; Deane, Jennifer; O'Connor, Michael; Harnedy, Norma; O'Connor, Kieran; O'Mahony, Denis; van Sinderen, Douwe; Wallace, Martina; Brennan, Lorraine; Stanton, Catherine
Abstract:
Alterations in intestinal microbiota composition are associated with several chronic conditions, including obesity and inflammatory diseases. The microbiota of older people displays greater inter-individual variation than that of younger adults. Here we show that the faecal microbiota composition from 178 elderly subjects formed groups, correlating with residence location in the community, day-hospital, rehabilitation or in long-term residential care. However, clustering of subjects by diet separated them by the same residence location and microbiota groupings. The separation of microbiota composition significantly correlated with measures of frailty, co-morbidity, nutritional status, markers of inflammation and with metabolites in faecal water. The individual microbiota of people in long-stay care was significantly less diverse than that of community dwellers. Loss of community-associated microbiota correlated with increased frailty. Collectively, the data support a relationship be...
http://hdl.handle.net/10468/10653
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How useful are the recommended counts and indices in the systematics of the octopodidae (mollusca: cephalopoda)
(2018)
Allcock, A. Louise; STRUGNELL, JAN M.; JOHNSON, MARK P.
How useful are the recommended counts and indices in the systematics of the octopodidae (mollusca: cephalopoda)
(2018)
Allcock, A. Louise; STRUGNELL, JAN M.; JOHNSON, MARK P.
http://hdl.handle.net/10379/10220
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Phylogenetic position of the coral symbiont ostreobium (ulvophyceae) inferred from chloroplast genome data
(2018)
Verbruggen, Heroen; Marcelino, Vanessa R.; Guiry, Michael D.; Cremen, Ma. Chiela M.; Ja...
Phylogenetic position of the coral symbiont ostreobium (ulvophyceae) inferred from chloroplast genome data
(2018)
Verbruggen, Heroen; Marcelino, Vanessa R.; Guiry, Michael D.; Cremen, Ma. Chiela M.; Jackson, Christopher J.
Abstract:
The green algal genus Ostreobium is an important symbiont of corals, playing roles in reef decalcification and providing photosynthates to the coral during bleaching events. A chloroplast genome of a cultured strain of Ostreobium was available, but low taxon sampling and Ostreobium's early-branching nature left doubt about its phylogenetic position. Here, we generate and describe chloroplast genomes from four Ostreobium strains as well as Avrainvillea mazei and Neomeris sp., strategically sampled earlybranching lineages in the Bryopsidales and Dasycladales respectively. At 80,584 bp, the chloroplast genome of Ostreobium sp. HV05042 is the most compact yet found in the Ulvophyceae. The Avrainvillea chloroplast genome is similar to 94 kbp and contains introns in infA and cysT that have nearly complete sequence identity except for an open reading frame (ORF) in infA that is not present in cysT. In line with other bryopsidalean species, it also contains regions with possibly bacter...
http://hdl.handle.net/10379/14299
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Place of articulation of anterior nasal versus oral stops in Croatian
(2015)
Liker, Marko; Gibbon, Fiona E.
Place of articulation of anterior nasal versus oral stops in Croatian
(2015)
Liker, Marko; Gibbon, Fiona E.
Abstract:
The purpose of this investigation is to analyse the place of articulation of anterior nasal versus oral stops in Croatian. Although there is agreement that placement for /n/ and /t d/ is in the anterior region, there is disagreement among different authors about the precise place of articulation for these sounds. Some authors view these targets as sharing identical placement while others view placement of /n/ as more posterior to /t d/. In this paper we use electropalatography (EPG) to investigate whether placement for these sounds is the same or different. The speech of six participants was recorded for the purposes of this study. The speech material consisted of 972 VCV sequences (V = /i a u/, C = /n t d/). Four EPG indices were analysed: the ACoG measure, the amount of contact at dental and alveolar articulatory zones (dentoalveolar articulation being inferred indirectly), incomplete EPG closures and the lateral contact measure. Coarticulatory effects of vowels on placement were ...
http://hdl.handle.net/10468/5704
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Protein and microsatellite single locus variability in salmo salar l. (atlantic salmon)
(2018)
Sánchez, J A; Clabby, C; Ramos, D; Blanco, G; Flavin, F; Vázquez, E; Powell, R
Protein and microsatellite single locus variability in salmo salar l. (atlantic salmon)
(2018)
Sánchez, J A; Clabby, C; Ramos, D; Blanco, G; Flavin, F; Vázquez, E; Powell, R
Abstract:
We describe genetic variation at three microsatellite single loci and six allozyme loci of seven natural Atlantic salmon populations from Ireland and Spain. A comparison of genetic variability detected at both types of loci is performed. Also, the relative value of microsatellite single locus variability with regard to the identification of individual salmon populations is assessed. Microsatellite loci are shown to display higher levels of variation than allozyme loci. Mean number of alleles (6+/-1.53) and heterozygosity (0.46+/-0.04) at microsatellite loci are greater than those found for allozymes (1.85+/-0.05 and 0.21+/-0.03, respectively), and some microsatellite alleles appear to be specific for a location or geographical area. Allozyme and microsatellite variation show the same pattern of differentiation between populations with Irish and Spanish populations grouped into different clusters. However, greater values of genetic distance were found among microsatellite (D=0.0747+/...
http://hdl.handle.net/10379/9841
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Rhizospheric bacterial community of endemic rhododendron arboreum sm. ssp. delavayi along eastern himalayan slope in tawang
(2018)
Debnath, Rajal; Yadav, Archana; Gupta, Vijai K.; Singh, Bhim P.; Handique, Pratap J.; S...
Rhizospheric bacterial community of endemic rhododendron arboreum sm. ssp. delavayi along eastern himalayan slope in tawang
(2018)
Debnath, Rajal; Yadav, Archana; Gupta, Vijai K.; Singh, Bhim P.; Handique, Pratap J.; Saikia, Ratul
Abstract:
Information on rhizosphere microbiome of endemic plants from high mountain ecosystems against those of cultivated plantations is inadequate. Comparative bacterial profiles of endemic medicinal plant Rhododendron arboreum Sm. subsp. delavayi rhizosphere pertaining to four altitudinal zonation Pankang Thang (PTSO), Nagula, Y-junction and Bum La (Indo-China border; in triplicates each) along cold adapted Eastern slope of Himalayan Tawang region, India is described here. Significant differences in DGGE profile between below ground bulk vs. rhizospheric community profile associated with the plant was identified. Tagged 16S amplicon sequencing from PTSO (3912 m) to Burn La (4509 m), revealed that soil pH, total nitrogen (TN), organic matter (OM) significantly influenced the underlying bacterial community structure at different altitudes. The relative abundance of Acidobacteria was inversely related to pH, as opposed to TN which was positively correlated to Acidobacteria and Proteobacteria...
http://hdl.handle.net/10379/11113
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The Bailey chain and mock theta functions
(2016)
Lovejoy, Jeremy; Osburn, Robert
The Bailey chain and mock theta functions
(2016)
Lovejoy, Jeremy; Osburn, Robert
Abstract:
Standard applications of the Bailey chain preserve mixed mock modularity but not mock modularity. After illustrating this with some examples, we show how to use a change of base in Bailey pairs due to Bressoud, Ismail and Stanton to explicitly construct families of q-hypergeometric multisums which are mock theta functions. We also prove identities involving some of these multisums and certain classical mock theta functions.
Science Foundation Ireland
http://hdl.handle.net/10197/7812
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The ccap knowledgebase: linking protistan and cyanobacterial biological resources with taxonomic and molecular data
(2018)
Gachon, Claire M.M.; Heesch, Svenja; Küpper, Frithjof C.; Achilles-Day, Undine E.M.; Br...
The ccap knowledgebase: linking protistan and cyanobacterial biological resources with taxonomic and molecular data
(2018)
Gachon, Claire M.M.; Heesch, Svenja; Küpper, Frithjof C.; Achilles-Day, Undine E.M.; Brennan, Debra; Campbell, Christine N.; Clarke, Alison; Dorrell, Richard G.; Field, Joanne; Gontarek, Steven; Rad Menendez, Cecilia; Saxon, Rachel J.; Veszelovszki, Andrea; Guiry, Michael D.; Gharbi, Karim; Blaxter, Mark; Day, John G.
Abstract:
The roles of Biological Resource Centres (BRCs), such as the Culture Collection of Algae and Protozoa (CCAP), have extended beyond their traditional maintenance and provision of curated microorganisms to the user community. A major driver for change has been the exponential increase in metagenomics and environmental sequencing data over the last few years. This has underlined a critical requirement for molecular information on reference biological materials, which would allow better taxonomic interpretation, greater biological understanding and additional exploitation of these data. This is especially relevant for the relatively poorly studied protists (algal/autotrophic as well as heterotrophic) and prokaryotic cyanobacteria, which despite their huge biodiversity, reflected in the genomic data that has been generated, are under-represented in BRCs worldwide. Here we describe the functionalities of the Culture Collection of Algae and Protozoa (CCAP) KnowledgeBase (http://www.ccap.ac...
http://hdl.handle.net/10379/11570
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The genome of leishmania adleri from a mammalian host highlights chromosome fission in sauroleishmania
(2018)
Coughlan, Simone; Mulhair, Peter; Sanders, Mandy; Schonian, Gabriele; Cotton, James A.;...
The genome of leishmania adleri from a mammalian host highlights chromosome fission in sauroleishmania
(2018)
Coughlan, Simone; Mulhair, Peter; Sanders, Mandy; Schonian, Gabriele; Cotton, James A.; Downing, Tim
Abstract:
Control of pathogens arising from humans, livestock and wild animals can be enhanced by genomebased investigation. Phylogenetically classifying and optimal construction of these genomes using short sequence reads are key to this process. We examined the mammal-infecting unicellular parasite Leishmania adleri belonging to the lizard-infecting Sauroleishmania subgenus. L. adleri has been associated with cutaneous disease in humans, but can be asymptomatic in wild animals. We sequenced, assembled and investigated the L. adleri genome isolated from an asymptomatic Ethiopian rodent ( MARV/ET/75/HO174) and verified it as L. adleri by comparison with other Sauroleishmania species. Chromosome-level scaffolding was achieved by combining reference-guided with de novo assembly followed by extensive improvement steps to produce a final draft genome with contiguity comparable with other references. L. tarentolae and L. major genome annotation was transferred and these gene models were manually v...
http://hdl.handle.net/10379/10934
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The gut microbiota composition in dichorionic triplet sets suggests a role for host genetic factors
(2015)
Murphy, Kiera; O'Shea, Carol Anne; Ryan, C. Anthony; Dempsey, Eugene M.; O'To...
The gut microbiota composition in dichorionic triplet sets suggests a role for host genetic factors
(2015)
Murphy, Kiera; O'Shea, Carol Anne; Ryan, C. Anthony; Dempsey, Eugene M.; O'Toole, Paul W.; Stanton, Catherine; Ross, R. Paul
Abstract:
Monozygotic and dizygotic twin studies investigating the relative roles of host genetics and environmental factors in shaping gut microbiota composition have produced conflicting results. In this study, we investigated the gut microbiota composition of a healthy dichorionic triplet set. The dichorionic triplet set contained a pair of monozygotic twins and a fraternal sibling, with similar pre- and post-natal environmental conditions including feeding regime. V4 16S rRNA and rpoB amplicon pyrosequencing was employed to investigate microbiota composition, and the species and strain diversity of the culturable bifidobacterial population was also examined. At month 1, the monozygotic pair shared a similar microbiota distinct to the fraternal sibling. By month 12 however, the profile was more uniform between the three infants. Principal coordinate analysis (PCoA) of the microbiota composition revealed strong clustering of the monozygotic pair at month 1 and a separation of the fraternal ...
http://hdl.handle.net/10468/2310
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The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice
(2013)
Zhang, Yuanbao; Wei, Chao; Jiang, Wendi; Wang, Lei; Li, Churui; Wang, Yunyue; Dow, J. M...
The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice
(2013)
Zhang, Yuanbao; Wei, Chao; Jiang, Wendi; Wang, Lei; Li, Churui; Wang, Yunyue; Dow, J. Maxwell; Sun, Wenxian
Abstract:
Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most important diseases in rice. However, little is known about the pathogenicity mechanisms of Xoc. Here we have investigated the function of three HD-GYP domain regulatory proteins in biofilm formation, the synthesis of virulence factors and virulence of Xoc. Deletion of rpfG resulted in altered production of extracellular polysaccharides (EPS), abolished virulence on rice and enhanced biofilm formation, but had little effect on the secretion of proteases and motility. In contrast, mutational analysis showed that the other two HD-GYP domain proteins had no effect on virulence factor synthesis and tested phenotypes. Mutation of rpfG led to up-regulation of the type III secretion system and altered expression of three putative glycosyltransferase genes gumD, pgaC and xagB, which are part of operons directing the synthesis of different extracellular polysaccharides. The pgaABCD and xagABCD operons we...
http://hdl.handle.net/10468/2386
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Institution
NUI Galway (13)
University College Cork (8)
University College Dublin (1)
Peer Review Status
Peer-reviewed (10)
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Year
2019 (1)
2018 (11)
2017 (2)
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