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Displaying Results 1 - 25 of 29 on page 1 of 2
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A novel process for mutation detection using uracil DNA-glycosylase
(1998)
Vaughan, Patrick; McCarthy, Tommie V.
A novel process for mutation detection using uracil DNA-glycosylase
(1998)
Vaughan, Patrick; McCarthy, Tommie V.
Abstract:
A novel process is presented for the detection of known mutations-and polymorphisms in DNA. This process, termed glycosylase mediated polymorphism detection (GMPD) involves amplification of the target DNA using three normal dNTPs and a fourth modified dNTP, whose base is a substrate for a specific DNA-glycosylase once incorporated into the DNA. The work described here utilises uracil DNA-glycosylase as the specific glycosylase and dUTP as the modified dNTP, Primers are designed so that during extension, the position of the first uracil incorporated into the extended primers differs depending on whether a mutation is present or absent. Subsequent glycosylase excision of the uracil residues followed by cleavage of the apyrimidinic sites allows detection of the mutation in the amplified fragment as a fragment length polymorphism. Variation in the sizes of the fragment length polymorphisms generated, can be readily achieved through the use of inosine bases in place of adenine bases in t...
http://hdl.handle.net/10468/5037
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Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance
(2017)
Pajic, Natasa Z. Bubic; Todosijevic, Marija N.; Vuleta, Gordana M.; Cekic, Nebojsa D.; ...
Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance
(2017)
Pajic, Natasa Z. Bubic; Todosijevic, Marija N.; Vuleta, Gordana M.; Cekic, Nebojsa D.; Dobricic, Vladimir D.; Vucen, Sonja R.; Calija, Bojan R.; Lukic, Milica Z.; Ilic, Tanja M.; Savic, Snezana D.
Abstract:
Two types of biocompatible surfactants were evaluated for their capability to formulate skin-friendly/non-irritant microemulsions as vehicles for two poorly water-soluble model drugs differing in properties and concentrations: alkyl polyglucosides (decyl glucoside and caprylyl/capryl glucoside) and ethoxylated surfactants (glycereth-7-caprylate/caprate and polysorbate 80). Phase behavior, structural inversion and microemulsion solubilization potential for sertaconazole nitrate and adapalene were found to be highly dependent on the surfactants structure and HLB value. Performed characterization (polarized light microscopy, pH, electrical conductivity, rheological, FTIR and DSC measurements) indicated a formulation containing glycereth-7-caprylate/caprate as suitable for incorporation of both drugs, whereas alkyl polyglucoside-based systems did not exhibit satisfying solubilization capacity for sertaconazole nitrate. Further, monitored parameters were strongly affected by sertaconazol...
http://hdl.handle.net/10468/5493
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An injectable elastin-based gene delivery platform for dose-dependent modulation of angiogenesis and inflammation for critical limb ischemia
(2016)
Dash, Biraja C.; Thomas, Dilip; Monaghan, Michael; Carroll, Oliver; Chen, Xizhe; Woodho...
An injectable elastin-based gene delivery platform for dose-dependent modulation of angiogenesis and inflammation for critical limb ischemia
(2016)
Dash, Biraja C.; Thomas, Dilip; Monaghan, Michael; Carroll, Oliver; Chen, Xizhe; Woodhouse, Kimberly; O'Brien, Timothy; Pandit, Abhay
Abstract:
Critical limb ischemia is a major clinical problem. Despite rigorous treatment regimes, there has been only modest success in reducing the rate of amputations in affected patients. Reduced level of blood flow and enhanced inflammation are the two major pathophysiological changes that occur in the ischemic tissue. The objective of this study was to develop a controlled dual gene delivery system capable of delivering therapeutic plasmid eNOS and IL-10 in a temporal manner. In order to deliver multiple therapeutic genes, an elastin-like polypeptide (ELP) based injectable system was designed. The injectable system was comprised of hollow spheres and an in situ-forming gel scaffold of elastin-like polypeptide capable of carrying gene complexes, with an extended manner release profile. In addition, the ELP based injectable system was used to deliver human eNOS and IL-10 therapeutic genes in vivo. A subcutaneous dose response study showed enhanced blood vessel density in the treatment grou...
http://hdl.handle.net/10379/5625
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Bitterness in sodium caseinate hydrolysates: role of enzyme preparation and degree of hydrolysis
(2017)
O'Sullivan, Dara; Nongonierma, Alice B.; Fitzgerald, Richard J.
Bitterness in sodium caseinate hydrolysates: role of enzyme preparation and degree of hydrolysis
(2017)
O'Sullivan, Dara; Nongonierma, Alice B.; Fitzgerald, Richard J.
Abstract:
BACKGROUND Enzymatic hydrolysis of sodium caseinate (NaCas) may lead to the development of bitterness. Careful selection of hydrolysis conditions (i.e. enzyme preparation and duration) yielding different degrees of hydrolysis (DH) may aid in the development of low bitterness. RESULTS Eighteen NaCas hydrolysates were generated with four enzyme preparations (Alcalase 2.4L, Prolyve 1000, FlavorPro Whey and pepsin) to different DH values. Hydrolysate bitterness score, assessed using a trained panel (ten assessors), generally increased at higher DH values for Alcalase, Prolyve and pepsin hydrolysates. However, all FlavorPro Whey hydrolysates (DH 0.38–10.62%) displayed low bitterness score values (<26.0%) comparable to that of intact NaCas (13.8 ± 2.0%, P > 0.05). CONCLUSION Enzyme preparation and DH affect the bitterness of NaCas hydrolysates. The results are relevant for the generation of NaCas hydrolysates with reduced bitterness.
ACCEPTED
peer-reviewed
http://hdl.handle.net/10344/5732
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Blue-light inhibition of listeria monocytogenes growth is mediated by reactive oxygen species and is influenced by σband the blue-light sensor lmo0799
(2018)
O'Donoghue, Beth; NicAogáin, Kerrie; Bennett, Claire; Conneely, Alan; Tiensuu, Ter...
Blue-light inhibition of listeria monocytogenes growth is mediated by reactive oxygen species and is influenced by σband the blue-light sensor lmo0799
(2018)
O'Donoghue, Beth; NicAogáin, Kerrie; Bennett, Claire; Conneely, Alan; Tiensuu, Teresa; Johansson, Jörgen; O'Byrne, Conor
Abstract:
Listeria monocytogenes senses blue light via the flavin mononucleotide-containing sensory protein Lmo0799, leading to activation of the general stress response sigma factor SigB (sigma(B)). In this study, we investigated the physiological response of this foodborne pathogen to blue light. We show that blue light (460 to 470 nm) doses of 1.5 to 2 mW cm(-2) cause inhibition of growth on agar-based and liquid culture media. The inhibitory effects are dependent on cell density, with reduced effects evident when high cell numbers are present. The addition of 20 mM dimethylthiourea, a scavenger of reactive oxygen species, or catalase to the medium reverses the inhibitory effects of blue light, suggesting that growth inhibition is mediated by the formation of reactive oxygen species. A mutant strain lacking sigma(B) (Delta sigB) was found to be less inhibited by blue light than the wild type, likely indicating the energetic cost of deploying the general stress response. When a lethal dose ...
http://hdl.handle.net/10379/13222
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Characterisation, solubility and intrinsic dissolution behaviour of benzamide: dibenzyl sulfoxide cocrystal
(2012)
Grossjohann, Christine; Eccles, Kevin S.; Maguire, Anita R.; Lawrence, Simon E.; Tajber...
Characterisation, solubility and intrinsic dissolution behaviour of benzamide: dibenzyl sulfoxide cocrystal
(2012)
Grossjohann, Christine; Eccles, Kevin S.; Maguire, Anita R.; Lawrence, Simon E.; Tajber, Lidia; Corrigan, Owen I.; Healy, Anne Marie
Abstract:
This study examined the 1:1 cocrystal benzamide: dibenzyl sulfoxide, comprising the poorly water soluble dibenzyl sulfoxide (DBSO) and the more soluble benzamide (BA), to establish if this cocrystal shows advantages in terms of solubility and dissolution in comparison to its pure components and to a physical mixture. Solubility studies were performed by measuring DBSO solubility as a function of BA concentration, and a ternary phase diagram was constructed. Dissolution was examined through intrinsic dissolution studies. Solid-state characterisation was carried out by powder X-ray diffraction (PXRD), energy-dispersive X-ray diffraction (EDX), infra-red spectroscopy (ATR-FTIR) and thermal analysis. DBSO solubility was increased by means of complexation with BA. For the cocrystal, the solubility of both components was decreased in comparison to pure components. The cocrystal was identified as metastable and incongruently saturating. Dissolution studies revealed that dissolution of DBSO...
http://hdl.handle.net/10468/921
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Chemical structure of the core region of campylobacter jejuni serotype o:2 lipopolysaccharide
(2018)
ASPINALL, Gerald O.; McDONALD, Armando G.; RAJU, T. Shantha; PANG, Henrianna; KURJANCZY...
Chemical structure of the core region of campylobacter jejuni serotype o:2 lipopolysaccharide
(2018)
ASPINALL, Gerald O.; McDONALD, Armando G.; RAJU, T. Shantha; PANG, Henrianna; KURJANCZYK, Linda A.; PENNER, John L.; MORAN, Anthony P.
Abstract:
The complete structure for the core region of Campylobacter jejuni serotype O:2 lipopolysaccharide (LPS) was assigned through studies on derivatives of the liberated oligosaccharide (OS 2) and the intact LPS. Structure determinations were performed using H-1-NMR spectroscopy, methylation studies supported by fast-atom-bombardment mass spectrometry and linkage analysis by gas chromatography/mass spectrometry, Smith degradation, and oxidation with chromium trioxide. It was concluded that complete oligosaccharide chains had the following structure: [GRAPHICS]
http://hdl.handle.net/10379/8841
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Comparative evaluation of biochemical changes in tomato (lycopersicon esculentum mill.) infected by alternaria alternata and its toxic metabolites (tea, aoh, and ame)
(2018)
Meena, Mukesh; Zehra, Andleeb; Dubey, Manish K.; Aamir, Mohd; Gupta, Vijai K.; Upadhyay...
Comparative evaluation of biochemical changes in tomato (lycopersicon esculentum mill.) infected by alternaria alternata and its toxic metabolites (tea, aoh, and ame)
(2018)
Meena, Mukesh; Zehra, Andleeb; Dubey, Manish K.; Aamir, Mohd; Gupta, Vijai K.; Upadhyay, Ram S.
Abstract:
In the present study, we have evaluated the comparative biochemical defense response generated against Alternaria alternate and its purified toxins viz. alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA). The necrotic lesions developed due to treatment with toxins were almost similar as those produced by the pathogen, indicating the crucial role of these toxins in plant pathogenesis. An oxidative burst reaction characterized by the rapid and transient production of a large amount of reactive oxygen species (ROS) occurs following the pathogen infection/toxin exposure. The maximum concentration of hydrogen peroxide (H2O2) produced was reported in the pathogen infected samples (22.2-fold) at 24 h post inoculation followed by TeA (18.2-fold), AOH (15.9-fold), and AME (14.1-fold) in treated tissues. 3,3'-Diaminobenzidine staining predicted the possible sites of H2O2 accumulation while the extent of cell death was measured by Evans blue dye. The extent o...
http://hdl.handle.net/10379/12866
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Contactless, probeless and non-titrimetric determination of acid-base reactions using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)
(2018)
Ahmed, M. Rizwan; Vos, Bastiaan; McSweeney, Sean; Kruse, Jacob; Fitzpatrick, Dara
Contactless, probeless and non-titrimetric determination of acid-base reactions using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)
(2018)
Ahmed, M. Rizwan; Vos, Bastiaan; McSweeney, Sean; Kruse, Jacob; Fitzpatrick, Dara
Abstract:
pH determination is a routine measurement in scientific laboratories worldwide. Most major advances in pH measurement were made in the 19th and early 20th century. pH measurements are critical for the determination of acid base reactions. This study demonstrates how an acid-base reaction can be monitored without the use of a pH probe, indicator and titres of reagent. The stoichiometric reaction between carbonate and HCl acid yields specific quantities of CO2, which causes reproducible changes to the compressibility of the solvent. This in turn slows down the speed of sound in solution which is induced by a magnetic follower gently tapping the inner wall of the vessel. As a consequence the frequencies of the acoustic resonances in the vessel are reduced. This approach is called Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS) which harnesses this phenomenon for many applications. The acid-carbonate experiments have also been validated using H2SO4 acid and using both pota...
http://hdl.handle.net/10468/5440
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Evaluating a New CEM III/A Cement for Concretes Exposed to Harsh Acid Rich Environments
(2016)
Thompson, David; Holmes, Niall; Reddy, John
Evaluating a New CEM III/A Cement for Concretes Exposed to Harsh Acid Rich Environments
(2016)
Thompson, David; Holmes, Niall; Reddy, John
Abstract:
The Irish Annex to the European specification, performance, production and conformity of concrete, IS EN 206, recommends CEM III/B cement for acid rich environments containing between 66 to 80% GGBS. However, BS 8500, the UK annex to EN 206 and the British Research Establishment (BRE) Special Digest 1, recommends CEM III/A cement with a GGBS range of 36-65%. This project investigated the performance of a new CEM III/A cement produced by Ecocem Ireland in concretes exposed to such environments using an extensive suite of laboratory tests. In Ireland, up to €5.8bn will be invested to provide fresh drinking water and clean wastewater. Concrete deterioration in wastewater treatment systems is mostly caused by sulphates and sulphuric acids found in aggressive chemical additives used. Due to the constant operational nature of these facilities, poor concrete performance leads to shut-downs with serious environmental consequences. The Department of Agriculture requires that all farm based c...
https://arrow.dit.ie/engschcivcon/89
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Evaluation of the efficacy and safety of a marine-derived bacillus strain for use as an in-feed probiotic for newly weaned pigs
(2014)
Prieto, Maria Luz; O'Sullivan, Laurie; Tan, Shiau Pin; McLoughlin, Peter; Hughes, ...
Evaluation of the efficacy and safety of a marine-derived bacillus strain for use as an in-feed probiotic for newly weaned pigs
(2014)
Prieto, Maria Luz; O'Sullivan, Laurie; Tan, Shiau Pin; McLoughlin, Peter; Hughes, Helen; O'Donovan, Orla; Rea, Mary C.; Kent, Robert M.; Cassidy, Joseph P.; Gardiner, Gillian E.; Lawlor, Peadar G.
Abstract:
Forty eight individual pigs (8.7 ± 0.26 kg) weaned at 28 ± 1 d of age were used in a 22-d study to evaluate the effect of oral administration of a Bacillus pumilus spore suspension on growth performance and health indicators. Treatments (n = 16) were: (1) non-medicated diet; (2) medicated diet with apramycin (200 mg/kg) and pharmacological levels of zinc oxide (2,500 mg zinc/kg) and (3) B. pumilus diet (non-medicated diet + 10(10) spores/day B. pumilus). Final body weight and average daily gain tended to be lower (P = 0.07) and feed conversion ratio was worsened (P<0.05) for the medicated treatment compared to the B. pumilus treatment. Ileal E. coli counts were lower for the B. pumilus and medicated treatments compared to the non-medicated treatment (P<0.05), perhaps as a result of increased ileal propionic acid concentrations (P<0.001). However, the medicated treatment reduced fecal (P<0.001) and cecal (P<0.05) Lactobacillus counts and tended to reduce the total ceca...
http://hdl.handle.net/10468/2341
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Exploring the scope of asymmetric synthesis of β-Hydroxy-γ-lactams via Noyori-type reductions
(2016)
Lynch, Denis; Deasy, Rebecca E.; Clarke, Leslie-Ann; Slattery, Catherine N.; Khandavill...
Exploring the scope of asymmetric synthesis of β-Hydroxy-γ-lactams via Noyori-type reductions
(2016)
Lynch, Denis; Deasy, Rebecca E.; Clarke, Leslie-Ann; Slattery, Catherine N.; Khandavilli, Udaya Bhaskara Rao; Lawrence, Simon E.; Maguire, Anita R.; Magnus, Nicholas A.; Moynihan, Humphrey A.
Abstract:
Enantio- and diastereoselective hydrogenation of β-keto-γ-lactams with a ruthenium–BINAP catalyst, involving dynamic kinetic resolution, has been employed to provide a general, asymmetric approach to β-hydroxy-γ-lactams, a structural motif common to several bioactive compounds. Full conversion to the desired β-hydroxy-γ-lactams was achieved with high diastereoselectivity (up to >98% de) by addition of catalytic HCl and LiCl, while β-branching of the ketone substituent demonstrated a pronounced effect on the modest to excellent enantioselectivity (up to 97% ee) obtained.
http://hdl.handle.net/10468/3333
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Functional protein rich extracts from bovine and porcine hearts using acid or alkali solubilisation and isoelectric precipitation
(2018)
Tsermoula, Paraskevi; Virgili, Claudia; Ortega, Rodrigo G.; Mullen, Anne M.; Alvarez, C...
Functional protein rich extracts from bovine and porcine hearts using acid or alkali solubilisation and isoelectric precipitation
(2018)
Tsermoula, Paraskevi; Virgili, Claudia; Ortega, Rodrigo G.; Mullen, Anne M.; Alvarez, Carlos; O'Brien, Nora M.; O'Flaherty, Elisabeth A. A.; O'Neill, Eileen E.
Abstract:
Alkali solubilisation (ALS) was compared with acid solubilisation (ACS) for preparation of protein rich extracts from bovine and porcine hearts. ACS and ALS recovered 51.53%–55.74% of the total protein from bovine and porcine hearts. All extracts were rich in myofibrillar proteins with both treatments resulting in reductions in fat, collagen and cholesterol contents compared with starting materials. At 0% NaCl, ACS and ALS extracts had good gelling properties with the ALS gels having lower % cook loss. While treatments did not affect gel hardness, ACS extracts formed gel networks with higher storage modulus after heating and cooling. At 2% NaCl gel hardness, % cook loss and storage modulus values increased, with greater increases occurring for ACS extracts. The results show that ALS‐ and ACS‐based processes have potential to produce functional ingredients for processed meat products.
http://hdl.handle.net/10468/7322
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Greener synthesis using hydrogen peroxide in ethyl acetate of alicyclic ring-fused benzimidazoles and anti-tumour benzimidazolequinones
(2018)
Sweeney, Martin; Gurry, Michael; Keane, Lee-Ann J.; Aldabbagh, Fawaz
Greener synthesis using hydrogen peroxide in ethyl acetate of alicyclic ring-fused benzimidazoles and anti-tumour benzimidazolequinones
(2018)
Sweeney, Martin; Gurry, Michael; Keane, Lee-Ann J.; Aldabbagh, Fawaz
http://hdl.handle.net/10379/14088
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Investigation of phosphorus desorption from P-saturated alum sludge used as a substrate in constructed wetland
(2011)
Zhao, X.H.; Zhao, Y.Q.
Investigation of phosphorus desorption from P-saturated alum sludge used as a substrate in constructed wetland
(2011)
Zhao, X.H.; Zhao, Y.Q.
Abstract:
Phosphorus (P) desorption from P-saturated alum sludge, which was used as main substrate in a novel constructed wetland for wastewater treatment, was studied. Groups of batch experiments were designed and conducted to explore the efficiencies of P extraction using different acids (HCl, HNO3, H2SO4) and bases (NaOH, KOH). The results showed that either acid or base is efficient for P-extraction and the efficiency relied mainly on the concentrations of H+/OH-, not the type of acid or base. Considering the efficiency, price and safety of the acids and bases tested, H2SO4 was chosen as most suitable reagent for P-extraction. A Box-Behnken experimental design based on the response surface methodology (RSM) was applied to evaluate the optimum of H2SO4 extraction. The optimal condition for the mass of sludge, H2SO4 concentration and volume was 0.8 g, 0.063 M and 142 ml, respectively. At such optimal condition, the maximum P-extraction efficiency of 98.2 % was achieved. Additionally, most o...
http://hdl.handle.net/10197/3134
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Involvement of capsaicin-sensitive afferent nerves and cholecystokinin 2/gastrin receptors in gastroprotection and adaptation of gastric mucosa to helicobacter pylori-lipopolysaccharide
(2018)
Brzozowski, T.
Involvement of capsaicin-sensitive afferent nerves and cholecystokinin 2/gastrin receptors in gastroprotection and adaptation of gastric mucosa to helicobacter pylori-lipopolysaccharide
(2018)
Brzozowski, T.
Abstract:
Lipopolysaccharide (LPS) is one of the virulence factors in the Helicobacter pylori (Hp)-infected stomach, but it remains unknown whether single and prolonged pretreatment with HpLPS can affect the course of gastric damage induced by aspirin (ASA). We compared the effects of Hp-LPS with those induced by LPSs isolated from intestinal Bacteroides fragilis, Yersinia enterocolitica, and Campylobacter jejuni applied for 4 days on acute ASA-induced gastric lesions in rats. The area of ASA-induced gastric lesions, gastric blood flow (GBF), expression of mRNA and protein of leptin and plasma leptin, gastrin, interleukin-1beta, and tumor necrosis factor-alpha levels were examined. Single (once) or repeated (five times) i.p. injections of Hp-LPS (1 mg/kg) or intestinal LPSs failed to produce macroscopic gastric damage and did not affect the GBF when compared with vehicle. Hp-LPS injected repeatedly suppressed the gastric acid secretion, up-regulated leptin mRNA and protein, and increased plas...
http://hdl.handle.net/10379/8913
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Isolation of Lactobacilli with probiotic properties from the human stomach
(2008)
Ryan, Kieran A.; Jayaraman, Thevaraajan; Daly, Paul; Canchaya, Carlos; Curran, Susan; F...
Isolation of Lactobacilli with probiotic properties from the human stomach
(2008)
Ryan, Kieran A.; Jayaraman, Thevaraajan; Daly, Paul; Canchaya, Carlos; Curran, Susan; Fang, Fang; Quigley, Eamonn M.; O'Toole, Paul W.
Abstract:
Aims: Recent evidence suggests that the human gastric microbiota is much more diverse than previously thought. The aim of this study was to assess the potential for isolating lactobacilli from the human stomach.Methods and Results: Lactobacilli were selectively cultured from gastric biopsies from 12 patients undergoing routine endoscopy. Lactobacilli were present in four of 12 biopsies. We isolated, in total 10 different strains representing five species (Lactobacillus gasseri, L. fermentum, L. vaginalis, L. reuteri and L. salivarius). The 10 isolates varied greatly in their ability to inhibit the growth of two Gram-positive bacteria and two Gram-negative bacteria. Furthermore, the acid and bile resistance profiles of the 10 isolates spanned a wide range. Conclusions: Five different Lactobacillus species were cultured from human gastric biopsies for the first time. Significance and Impact of the Study: Diverse Lactobacillus species are more prevalent in the human stomach than previo...
http://hdl.handle.net/10468/116
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Measuring the Micro-Polarity and Hydrogen-Bond Donor/Acceptor Ability of Thermoresponsive N-Isopropylacrylamide/N-tert-Butylacrylamide Copolymer Films Using Solvatochromic Indicators
(2014)
Ryder, Alan G.; Szczupak, Boguslaw; Togashi, Denisio M.; Rochev, Yuri A.; Glynn, Thomas J.
Measuring the Micro-Polarity and Hydrogen-Bond Donor/Acceptor Ability of Thermoresponsive N-Isopropylacrylamide/N-tert-Butylacrylamide Copolymer Films Using Solvatochromic Indicators
(2014)
Ryder, Alan G.; Szczupak, Boguslaw; Togashi, Denisio M.; Rochev, Yuri A.; Glynn, Thomas J.
Abstract:
Thin polymer films are important in many areas of biomaterials research, biomedical devices, and biological sensors. The accurate in situ measurement of multiple physicochemical properties of thin polymer films is critical in understanding biocompatibility. polymer function, and performance. In this work we demonstrate a facile spectroscopic methodology for accurately measuring the micro-polarity and hydrogen-bond donor/acceptor ability for a series of relatively hydrophilic thermoresponsive copolymers. The micro-polarity of the N-isopropylacrylamide (NIPAM) and N-tert-butylacrylamide (NtBA) co-polymers was evaluated by means of the E-T(30), alpha, beta, and pi* empirical solvatochromic polarity parameters. The data shows that increasing the NtBA fraction in the dry copolymer film reduces polarity and hydrogen-bonding ability. Within the Kamlet-Taft polarity, framework, the NIPAM/NtBA copolymer films are strong hydrogen-bond acceptors. strongly dipolar/polarizable and rather moderat...
http://hdl.handle.net/10379/3983
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Multiple sequence alignment with the Clustal series of programs
(2003)
Chenna, Ramu; Sugawara, Hideaki; Koike, Tadashi; Lopez, Rodrigo; Gibson, Toby J.; Higg...
Multiple sequence alignment with the Clustal series of programs
(2003)
Chenna, Ramu; Sugawara, Hideaki; Koike, Tadashi; Lopez, Rodrigo; Gibson, Toby J.; Higgins, Desmond G.; Thompson, Julie D.
Abstract:
The Clustal series of programs are widely used in molecular biology for the multiple alignment of both nucleic acid and protein sequences and for preparing phylogenetic trees. The popularity of the programs depends on a number of factors, including not only the accuracy of the results, but also the robustness, portability and user-friendliness of the programs. New features include NEXUS and FASTA format output, printing range numbers and faster tree calculation. Although, Clustal was originally developed to run on a local computer, numerous Web servers have been set up, notably at the EBI (European Bioinformatics Institute) (http://www.ebi.ac.uk/clustalw/).
http://hdl.handle.net/10468/5035
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Ni nanowire supported 3D flower-like Pd nanostructures as an efficient electrocatalyst for electrooxidation of ethanol in alkaline media
(2012)
Newcomb, Simon B.; Hasan, Maksudul; Rohan, James F.; Razeeb, Kafil M.
Ni nanowire supported 3D flower-like Pd nanostructures as an efficient electrocatalyst for electrooxidation of ethanol in alkaline media
(2012)
Newcomb, Simon B.; Hasan, Maksudul; Rohan, James F.; Razeeb, Kafil M.
Abstract:
A Ni nanowire array (NiNWA) supported three-dimensional flower-like Pd nano-electrocatalyst with high electrocatalytic performance for the electrooxidation of ethanol in alkaline media has been fabricated by borohydride hydrothermal reduction method. This novel hybrid NiNWA/PdNF (nanoflowers) electrocatalyst exhibits large electrochemically active surface area (EASA, 45 m2 g−1(Pd)), excellent electrocatalytic activity (765 mA mg−1(Pd)), and high level of the poisoning tolerance (If/Ib = 1.2) to the carbonaceous oxidative intermediates for the electrooxidation reaction in alkaline media. In addition, the electrochemical stability of NiNWA/PdNF is significantly higher than that of NiNWA/PdNP (nanoparticles) electrocatalyst, as evidenced by chronoamperometry experiments in which the electrooxidation current of nanoflowers is controlled by the diffusion transport of ethanol species rather than the carbonaceous poisoning. This high electrocatalytic activity can be attributed to the more ...
http://hdl.handle.net/10468/7657
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Performance of the eu-harmonised mouse bioassay for lipophilic toxins for the detection of azaspiracids in naturally contaminated mussel (mytilus edulis) hepatopancreas tissue homogenates characterised by liquid chromatography coupled to tandem mass spectrometry
(2018)
Hess, Philipp; Butter, Tim; Petersen, Andrew; Silke, Joe; McMahon, Terry
Performance of the eu-harmonised mouse bioassay for lipophilic toxins for the detection of azaspiracids in naturally contaminated mussel (mytilus edulis) hepatopancreas tissue homogenates characterised by liquid chromatography coupled to tandem mass spectrometry
(2018)
Hess, Philipp; Butter, Tim; Petersen, Andrew; Silke, Joe; McMahon, Terry
http://hdl.handle.net/10379/11919
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Phase variation of campylobacter jejuni 81-176 lipooligosaccharide affects ganglioside mimicry and invasiveness in vitro
(2018)
Guerry, P.
Phase variation of campylobacter jejuni 81-176 lipooligosaccharide affects ganglioside mimicry and invasiveness in vitro
(2018)
Guerry, P.
Abstract:
The outer cores of the lipooligosaccharides (LOS) of many strains of Campylobacter jejuni mimic human gangliosides in structure. A population of cells of C.jejuni strain 81-176 produced a mixture of LOS cores which consisted primarily of structures mimicking GM(2) and GM(3) gangliosides, with minor amounts of structures mimicking GD(11) and GD(2). Genetic analyses of genes involved in the biosynthesis of the outer core of C. Jejuni 81-176 revealed the presence of a homopolymeric tract of G residues within a gene encoding CgtA, an N-acetylgalactosaminyltransferase. Variation in the number of G residues within cgtA affected the length of the open reading frame, and these changes in cgtA corresponded to a change in LOS structure from GM(2) to GM(3) ganglioside mimicry. Site-specific mutation of cgtA in 81-176 resulted in a major LOS core structure that lacked GaINAc and resembled GM(3) ganglioside. Compared to wild-type 81-176, the cgtA mutant showed a significant increase in invasion ...
http://hdl.handle.net/10379/9254
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Physicochemical and sensory characteristics of whey protein hydrolysates generated at different total solids levels
(2018)
Spellman, David; O'Cuinn, Gerard; FitzGerald, Richard J
Physicochemical and sensory characteristics of whey protein hydrolysates generated at different total solids levels
(2018)
Spellman, David; O'Cuinn, Gerard; FitzGerald, Richard J
Abstract:
Whey protein hydrolysates were generated at different total solids (TS) levels (50-300 g/l) using the commercially available proteolytic preparation Debitrase™ HYW20, while enzyme to substrate ratio, pH and temperature were maintained constant. Hydrolysis proceeded at a faster rate at lower TS reaching a degree of hydrolysis (DH) of 16-6% at 300 g TS/l, compared with a DH of 22-7% at 50 g TS/l after 6 h hydrolysis. The slower breakdown of intact whey proteins at high TS was quantified by gel-permeation HPLC. Reversed-phase (RP) HPLC of hydrolysate samples of equivalent DH (&SIM; 15%) generated at different TS levels indicated that certain hydrophobic peptide peaks were present at higher levels in hydrolysates generated at low TS. Sensory evaluation showed that hydrolysates with equivalent DH values were significantly (P< 0(.)0005) less bitter when generated at 300 g TS/l (mean bitterness score=25(.)4%) than hydrolysates generated at 50 g TS/l (mean bitterness sc...
http://hdl.handle.net/10379/9899
Marked
Mark
Protective role of exogenous phytohormones on redox status in pea seedlings under copper stress
(2017)
Ben Massoud, Marouane; Sakouhi, Lamia; Karmous, Inès; Zhu, Yao; El Ferjani, Ezzedine; S...
Protective role of exogenous phytohormones on redox status in pea seedlings under copper stress
(2017)
Ben Massoud, Marouane; Sakouhi, Lamia; Karmous, Inès; Zhu, Yao; El Ferjani, Ezzedine; Sheehan, David; Chaoui, Abdelilah
Abstract:
The present work aims to provide insight on the role of phytohormone application in developing efficient practical defense strategies to improve plants tolerance under heavy metal contamination. For this purpose, pea (Pisum sativum L.) seeds were germinated in an aqueous solution of 200 mu M CuCl2 up to the 3rd day and then continued to germinate in the presence of distilled water (stress cessation) or were subjected to following combinations: Cu + 1 mu M IAA and Cu + 1 mu M GA(3) for 3 additional days. The results showed that copper excess induced oxidative stress in germinating seeds, which resulted in changes of the redox state of glutathione and cysteine, and proteomics revealed Cu-induced modifications of thiols (SH) and carbonyls (CO) (indicators of protein oxidation). However, application of IAA or GA(3) in the germination medium after 3 days of Cu exposure alleviated toxicity on seedlings, despite the persistence of Cu up to 6th day. This improving effect seems to be mediate...
http://hdl.handle.net/10468/5865
Marked
Mark
Purification and characterization of a lysine-p-nitroanilide hydrolase, a broad specificity aminopeptidase, from the cytoplasm of lactococcus lactis subsp. cremoris am2
(2018)
McDONNELL, MAEVE; BOUCHIER, PAUL; FITZGERALD, RICHARD J.; O'CUINN, GERARD
Purification and characterization of a lysine-p-nitroanilide hydrolase, a broad specificity aminopeptidase, from the cytoplasm of lactococcus lactis subsp. cremoris am2
(2018)
McDONNELL, MAEVE; BOUCHIER, PAUL; FITZGERALD, RICHARD J.; O'CUINN, GERARD
Abstract:
a hydrolase activity that cleaves lysyl-p-nitroanilide (Lys-pNA) has been purified from the cytoplasm of Lactococcus lactis subsp. cremoris AM2 by chromatography on DE52, DEAE Affi-Gel Blue Gel, Hydroxyapatite Bio-Gel HTP and Phenyl Sepharose. The purified aminopeptidase was found to have a native M-r of 50 000-55 000 by gel filtration chromatography and by FPLC gel filtration on Superose 12 and to be composed of a single polypeptide chain following SDS-PAGE. Enzyme activity was almost completely inhibited by EDTA, amastatin, puromycin and bestatin, while the sulphydryl-reactive agents p-chloromercuribenzoate and iodoacetamide were inhibitory. The enzyme was found to be very unstable during the purification procedures at 4 degrees C and its stability was greatly improved when 10 ml glycerol/l and 2 mM-dithiothreitol were included in the purification buffers. The purified enzyme was found to hydrolyse a wide range of dipeptides. tripeptides and longer peptides provided that proline w...
http://hdl.handle.net/10379/9495
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