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Subject = lactic acid bacteria;
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Displaying Results 1 - 25 of 34 on page 1 of 2
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“Green preservatives” – combating fungi in the food industry by applying antifungal lactic acid bacteria
(2013)
Pawlowska, Agata
“Green preservatives” – combating fungi in the food industry by applying antifungal lactic acid bacteria
(2013)
Pawlowska, Agata
Abstract:
Fungal spoilage is the most common type of microbial spoilage in food leading to significant economical and health problems throughout the world. Fermentation by lactic acid bacteria (LAB) is one of the oldest and most economical methods of producing and preserving food. Thus, LAB can be seen as an interesting tool in the development of novel bio-preservatives for food industry. The overall objective of this study was to demonstrate, that LAB can be used as a natural way to improve the shelf-life and safety of a wide range of food products. In the first part of the thesis, 116 LAB isolates were screened for their antifungal activity against four Aspergillus and Penicillium spp. commonly found in food. Approximately 83% of them showed antifungal activity, but only 1% showed a broad range antifungal activity against all tested fungi. The second approach was to apply LAB antifungal strains in production of food products with extended shelf-life. L. reuteri R29 strain was identified as ...
http://hdl.handle.net/10468/1510
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A plasmid-encoded putative glycosyltransferase is involved in hop tolerance and beer spoilage in Lactobacillus brevis
(2020)
Feyereisen, Marine; Mahony, Jennifer; O'Sullivan, Tadhg; Boer, Viktor; van Sindere...
A plasmid-encoded putative glycosyltransferase is involved in hop tolerance and beer spoilage in Lactobacillus brevis
(2020)
Feyereisen, Marine; Mahony, Jennifer; O'Sullivan, Tadhg; Boer, Viktor; van Sinderen, Douwe
Abstract:
Lactobacillus brevis beer-spoiling strains harbor plasmids that contain genes such as horA, horC, and hitA which are known to confer hop tolerance. The L. brevis beer-spoiling strain UCCLBBS124, which possesses four plasmids, was treated with novobiocin, resulting in the isolation of UCCLBBS124 derivatives exhibiting hop sensitivity and an inability to grow in beer. One selected derivative was shown to have lost a single plasmid, here designated UCCLBBS124_D, which harbors the UCCLBBS124_pD0015 gene, predicted to encode a glycosyltransferase. Hop tolerance and growth in beer were restored when UCCLBBS124_pD0015 was introduced in one of these hop-sensitive derivatives on a plasmid. We hypothesize that this gene modifies the surface composition of the polysaccharide cell wall, conferring protection against hop compounds. Furthermore, the introduction of this gene in trans in L. brevis UCCLB521, a strain that cannot grow in and spoil beer, was shown to furnish the resulting strain with...
http://hdl.handle.net/10468/9955
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Antimicrobial antagonists against food pathogens; a bacteriocin perspective
(2015)
O'Connor, Paula M.; Ross, R Paul; Hill, Colin; Cotter, Paul D.
Antimicrobial antagonists against food pathogens; a bacteriocin perspective
(2015)
O'Connor, Paula M.; Ross, R Paul; Hill, Colin; Cotter, Paul D.
Abstract:
Efforts are continuing to find novel bacteriocins with enhanced specificity and potency. Traditional plating techniques are still being used for bacteriocin screening studies, however, the availability of ever more bacterial genome sequences and the use of in silico gene mining tools have revealed novel bacteriocin gene clusters that would otherwise have been overlooked. Furthermore, synthetic biology and bioengineering-based approaches are allowing scientists to harness existing and novel bacteriocin gene clusters through expression in different hosts and by enhancing functionalities. The same principles apply to bacteriocin producing probiotic cultures and their application to control pathogens in the gut. We can expect that the recent developments on bacteriocins from Lactic Acid Bacteria (LAB) described here will contribute greatly to increased commercialisation of bacteriocins in food systems.
This work was funded by the Alimentary Pharmabiotic Centre, a research centre fun...
http://hdl.handle.net/11019/784
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Bacteriocin Gene-Trait matching across the complete Lactobacillus Pan-genome
(2017)
Collins, Fergus W. J.; O'Connor, Paula M.; O'Sullivan, Orla; Gómez-Sala, Beat...
Bacteriocin Gene-Trait matching across the complete Lactobacillus Pan-genome
(2017)
Collins, Fergus W. J.; O'Connor, Paula M.; O'Sullivan, Orla; Gómez-Sala, Beatriz; Rea, Mary C.; Hill, Colin; Ross, R. Paul
Abstract:
Lactobacilli constitute a large genus of Gram-positive lactic acid bacteria which have widespread roles ranging from gut commensals to starters in fermented foods. A combination of in silico and laboratory-based screening allowed us to determine the overall bacteriocin producing potential of representative strains of each species of the genus. The genomes of 175 lactobacilli and 38 associated species were screened for the presence of antimicrobial producing genes and combined with screening for antimicrobial activity against a range of indicators. There also appears to be a link between the strains’ environment and bacteriocin production, with those from the animal and human microbiota encoding over twice as many bacteriocins as those from other sources. Five novel bacteriocins were identified belonging to differing bacteriocin classes, including two-peptide bacteriocins (muricidin and acidocin X) and circular bacteriocins (paracyclicin). In addition, there was a clear clustering of...
http://hdl.handle.net/10468/4181
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Biodiversity and classification of phages infecting Lactobacillus brevis
(2019)
Feyereisen, Marine; Mahony, Jennifer; Neve, Horst; Franz, Charles M. A. P.; Noben, Jean...
Biodiversity and classification of phages infecting Lactobacillus brevis
(2019)
Feyereisen, Marine; Mahony, Jennifer; Neve, Horst; Franz, Charles M. A. P.; Noben, Jean-Paul; O'Sullivan, Tadhg; Boer, Viktor; van Sinderen, Douwe
Abstract:
Lactobacillus brevis is a lactic acid bacterium that is known as a food and beverage spoilage organism, and more specifically as a beer-spoiler. Phages of L. brevis have been described, but very limited data is available regarding temperate phages of L. brevis. Temperate phages may exert benefits to the host, while they may also be employed to combat beer spoilage. The current study reports on the incidence of prophage sequences present in nineteen distinct L. brevis genomes. Prophage induction was evaluated using mitomycin C exposure followed by genome targeted-PCR, electron microscopy and structural proteome analysis. The morphological and genome sequence analyses revealed significant diversity among L. brevis prophages, which appear to be dominated by members of the Myoviridae phage family. Based on this analysis, we propose a classification of L. brevis phages into five groups.
http://hdl.handle.net/10468/8836
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Characterization of beer-spoiling Lactobacillus brevis and their associated bacteriophages
(2019)
Feyereisen, Marine
Characterization of beer-spoiling Lactobacillus brevis and their associated bacteriophages
(2019)
Feyereisen, Marine
Abstract:
Certain bacteria have acquired the ability to survive and even grow in beer, despite the antimicrobial nature of this harsh liquid environment. Bacterial growth in beer is often attributed to lactic acid bacteria (LAB) that are found in many environments, yet are commonly associated with plant material. While most LAB species bestow positive attributes to foods and food fermentations, others have the potential to survive and even thrive in inhospitable environments, including beer. Lactobacillus brevis strains are among the most frequently encountered LAB isolates in spoiled beer. In finished beer products, the presence of Lb. brevis is considered undesirable as they typically impart undesirable organoleptic properties on the product including off-flavors, odors and changes in the physical appearance and viscosity. The goal of the current study was to identify and characterize the molecular players that allow certain Lb. brevis to grow in and spoil in beer. It was also aimed to isol...
http://hdl.handle.net/10468/10437
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Comparative genomics of lactic acid bacteria reveals a niche-specific gene set
(2012)
O'Sullivan, Orla; O'Callaghan, John; Sangrador-Vegas, Amaia; McAuliffe, Olivi...
Comparative genomics of lactic acid bacteria reveals a niche-specific gene set
(2012)
O'Sullivan, Orla; O'Callaghan, John; Sangrador-Vegas, Amaia; McAuliffe, Olivia; Slattery, Lydia; Kaleta, Pawel; Callanan, Michael J.; Fitzgerald, Gerald F; Ross, R Paul; Beresford, Tom
Abstract:
Background: The recently sequenced genome of Lactobacillus helveticus DPC4571 1 revealed a dairy organism with significant homology (75% of genes are homologous) to a probiotic bacteria Lb. acidophilus NCFM 2. This led us to hypothesise that a group of genes could be determined which could define an organism's niche. Results: Taking 11 fully sequenced lactic acid bacteria (LAB) as our target, (3 dairy LAB, 5 gut LAB and 3 multi-niche LAB), we demonstrated that the presence or absence of certain genes involved in sugar metabolism, the proteolytic system, and restriction modification enzymes were pivotal in suggesting the niche of a strain. We identified 9 niche specific genes, of which 6 are dairy specific and 3 are gut specific. The dairy specific genes identified in Lactobacillus helveticus DPC4571 were lhv_1161 and lhv_1171, encoding components of the proteolytic system, lhv_1031 lhv_1152, lhv_1978 and lhv_0028 encoding restriction endonuclease genes, while bile salt hydrolas...
http://hdl.handle.net/11019/247
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Conjugated linoleic acid production and probiotic assessment of Lactobacillus plantarum isolated from Pico cheese
(2019)
Ribeiro, Susana C.; STANTON, CATHERINE; Yang, Bo; Ross, R Paul; Silva, Célia C.G.
Conjugated linoleic acid production and probiotic assessment of Lactobacillus plantarum isolated from Pico cheese
(2019)
Ribeiro, Susana C.; STANTON, CATHERINE; Yang, Bo; Ross, R Paul; Silva, Célia C.G.
Abstract:
Lactic acid bacteria isolated from a traditional Azorean cheese were screened for their ability to convert free linoleic acid to conjugated linoleic acid (CLA). Two strains of Lactobacillus plantarum were recognized as potential CLA producers. GC analysis identified cis-9, trans-11 C18:2 as the predominant isomer (10–14 μg/mL), followed by trans-9, trans-11 C18:2 (4–6 μg/mL). The CLA producing strains demonstrated strong biofilm capacity, high cell surface hydrophobicity and good auto-aggregation ability. These strains were capable of surviving in the presence of bile salts (0.3%) and pancreatin (0.1%), but only the highest CLA producer (L3C1E8) was able to resist low pH (2.5). Moreover, the CLA-producers showed good adhesion capacity to intestinal human cells (Caco-2 and HT-29) and were able to prevent colonization of Escherichia coli. Of the two strains, Lactobacillus plantarum L3C1E8 revealed superior probiotic properties and great potential for producing food products enriched i...
http://hdl.handle.net/11019/1747
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Developing bacteriocins of lactic acid bacteria into next generation biopreservatives
(2018)
Field, Des; Ross, R. Paul; Hill, Colin
Developing bacteriocins of lactic acid bacteria into next generation biopreservatives
(2018)
Field, Des; Ross, R. Paul; Hill, Colin
Abstract:
Bacteriocins are ribosomally synthesized peptides produced by bacteria which can kill other bacteria. Those produced by lactic acid bacteria (LAB) are of great interest as they are often employed in food processing and food fermentations as natural biopreservatives. In this review, we discuss the implementation of bioengineering to enhance the antimicrobial activity, antibacterial spectrum and physico-chemical properties of LAB bacteriocins. Additionally, we discuss the potential applications of bacteriocin derivatives for use as promising food preservatives alone or in combination with other naturally derived antimicrobials as a form of hurdle technology and the regulatory status of strains engineered through food-grade approaches.
http://hdl.handle.net/10468/5545
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Development and Application of Strategies to Generate Bacteriophage Resistant Strains for Use in Milk Fermentation Processes
(2017)
Ross, R Paul; Fitzgerald, Gerald F; Coffey, Aidan; Coakley, M.; O'Sullivan, Daniel
Development and Application of Strategies to Generate Bacteriophage Resistant Strains for Use in Milk Fermentation Processes
(2017)
Ross, R Paul; Fitzgerald, Gerald F; Coffey, Aidan; Coakley, M.; O'Sullivan, Daniel
Abstract:
End of Project Report
The objectives of this project were firstly, the identification of natural phage resistance systems for exploitation, secondly, the development of methodologies to utilise these systems to improve the bacteriophage resistance of starter strains for use in milk fermentation processes, and thirdly, the actual application of these methodologies to improving starter strains. The main conclusions were as follows: Three new natural plasmid (DNA)-associated bacteriophage resistance systems were identified at Moorepark. The detailed genetic makeup of the phage resistance plasmid (pMRC01) was elucidated. Bacteriophages currently evolving in the industrial cheese-making environment were monitored to facilitate the judicious choice of phage resistance systems for use in commercial starter cultures which can more effectively target the documented problematic phage types. Two highly virulent phages targeting important cheese starters were identified in the industria...
http://hdl.handle.net/11019/1310
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Evaluation of microbial adjuncts and their effect on the ripening of cheddar cheese
(2013)
Ristagno, Diletta
Evaluation of microbial adjuncts and their effect on the ripening of cheddar cheese
(2013)
Ristagno, Diletta
Abstract:
A bacteriocin-producing strain of Lactobacillus paracasei DPC 4715 was used as an adjunct culture in Cheddar cheese in order to control the growth of “wild” nonstarter lactic acid bacteria. No suppression of growth of the indicator strain was observed in the experimental cheese. The bacteriocin produced by Lactobacillus paracasei DPC 4715 was sensitive to chymosin and cathepsin D and it may have been cleaved by the rennet used for the cheese manufactured or by indigenous milk proteases. A series of studies were performed using various microbial adjuncts to influence cheese ripening. Microbacterium casei DPC 5281, Corynebacterium casei DPC 5293 and Corynebacterium variabile DPC 5305 were added to the cheesemilk at level of 109 cfu/ml resulting in a final concentration of 108 cfu/g in Cheddar cheese. The strains significantly increased the level of pH 4.6-soluble nitrogen, total free amino acids after 60 and 180 d of ripening and some individual free amino acids after 180 d. Yarrowia ...
http://hdl.handle.net/10468/1390
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Functional application of lactic acid bacteria exopolysaccharide in complex food systems
(2015)
Zannini, Emanuele
Functional application of lactic acid bacteria exopolysaccharide in complex food systems
(2015)
Zannini, Emanuele
Abstract:
Lactic acid bacteria expolysaccharides (LAB-EPS), in particular those formed from sucrose have the potential to improve food and beverage rheology and enhance their sensory properties potentially replacing or reducing expensive hydrocolloids currently used as improvers in food and beverage industries. Addition of sucrose not only enables EPS formation but also affects organic acid formation, thus influencing the sensory properties of the resulting food/beverage products. The first part of the study the organoleptic modulation of barley malt derived wort fermented using in situ produced bacterial polysaccharides has been investigated. Weisella cibaria MG1 was capable to produce exopolysaccharides during sucrosesupplemented barley malt derived wort fermentation. Even though the strain dominated the (sucrose-supplemented) wort fermentation, it was found to produce EPS (14.4 g l-1) with lower efficiency than in SucMRS (34.6 g l-1). Higher maltose concentration in wort led to the increas...
http://hdl.handle.net/10468/2249
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Genetic tools for investigating the biology of commensal lactobacilli
(2009)
Fang, Fang; O'Toole, Paul W.
Genetic tools for investigating the biology of commensal lactobacilli
(2009)
Fang, Fang; O'Toole, Paul W.
Abstract:
Lactobacilli belong to the genus Lactobacillus, the largest genus among the lactic acid bacteria (LAB). They are abundant in plant material and food resources, or they may inhabit niches in or on the bodies of humans and animals, as commensals. Lactobacilli of food origin are commercially important in the production of dairy products, fermented meats, vegetables, and sourdough, and many of their properties have been well studied. Commensal lactobacilli are good candidates for development as probiotics. In recent years, the general biology and host interaction mechanisms of commensal lactobacilli have attracted great interest. Although the metabolic pathways, predicted gene functions, and some phenotypic traits, of commensal lactobacilli can be inferred or deduced to an extent by the growing number of Lactobacillus genome sequencing project, various genetic tools are still required to confirm their phenotypic properties and biological traits. The current state of the art with respect...
http://hdl.handle.net/10468/112
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Identification of dual receptor binding protein systems in Lactococcal 936 group phages
(2018)
Hayes, Stephen; Duhoo, Yoan; Neve, Horst; Murphy, James; Noben, Jean-Paul; Franz, Charl...
Identification of dual receptor binding protein systems in Lactococcal 936 group phages
(2018)
Hayes, Stephen; Duhoo, Yoan; Neve, Horst; Murphy, James; Noben, Jean-Paul; Franz, Charles M. A. P.; Cambillau, Christian; Mahony, Jennifer; Nauta, Arjen; van Sinderen, Douwe
Abstract:
Siphoviridae of the lactococcal 936 group are the most commonly encountered bacteriophages in the dairy processing environment. The 936 group phages possess a discrete baseplate at the tip of their tail—a complex harbouring the Receptor Binding Protein (RBP) which is responsible for host recognition and attachment. The baseplate-encoding region is highly conserved amongst 936 phages, with 112 of 115 publicly available phages exhibiting complete synteny. Here, we detail the three exceptions (Phi4.2, Phi4R15L, and Phi4R16L), which differ from this genomic architecture in possessing an apparent second RBP-encoding gene upstream of the “classical” rbp gene. The newly identified RBP possesses an elongated neck region relative to currently defined 936 phage RBPs and is genetically distinct from defined 936 group RBPs. Through detailed characterisation of the representative phage Phi4.2 using a wide range of complementary techniques, we demonstrated that the above-mentioned three phages po...
http://hdl.handle.net/10468/8106
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Isolation and characterisation of antifungal compounds from lactic acid bacteria and their application in wheat and gluten-free bread
(2015)
Axel, Claudia
Isolation and characterisation of antifungal compounds from lactic acid bacteria and their application in wheat and gluten-free bread
(2015)
Axel, Claudia
Abstract:
As part of the “free-from” trend, biopreservation for bread products has increasingly become important to prevent spoilage since artificial preservatives are more and more rejected by consumers. A literature review conducted as part of this thesis revealed that the evaluation of more suitable antifungal strains of lactic acid bacteria (LAB) is important. Moreover, increasing the knowledge about the origin of the antifungal effect is fundamental for further enhancement of biopreservation. This thesis addresses the investigation of Lactobacillus amylovorus DSM19280, Lb. brevis R2: and Lb. reuteri R29 for biopreservation using in vitro trials and in situ sourdough fermentations of quinoa, rice and wheat flours as biopreservatives in breads. Their contribution to quality and shelf life extension on bread was compared and related to their metabolic activity and substrate features. Moreover, the quantity of antifungal carboxylic acids produced during sourdough fermentation was analysed. O...
http://hdl.handle.net/10468/2127
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Lactic Acid Bacteria and Bifidobacteria with Potential to Design Natural Biofunctional Health-Promoting Dairy Foods
(2019)
Linares, Daniel M.; Gomez, Carolina; Renes, Erica; Fresno, José M.; Tornadijo, María E....
Lactic Acid Bacteria and Bifidobacteria with Potential to Design Natural Biofunctional Health-Promoting Dairy Foods
(2019)
Linares, Daniel M.; Gomez, Carolina; Renes, Erica; Fresno, José M.; Tornadijo, María E.; Ross, R Paul; STANTON, CATHERINE
Abstract:
Consumer interest in healthy lifestyle and health-promoting natural products is a major driving force for the increasing global demand of biofunctional dairy foods. A number of commercial sources sell synthetic formulations of bioactive substances for use as dietary supplements. However, the bioactive-enrichment of health-oriented foods by naturally occurring microorganisms during dairy fermentation is in increased demand. While participating in milk fermentation, lactic acid bacteria can be exploited in situ as microbial sources for naturally enriching dairy products with a broad range of bioactive components that may cover different health aspects. Several of these bioactive metabolites are industrially and economically important, as they are claimed to exert diverse health-promoting activities on the consumer, such as anti-hypertensive, anti-inflammatory, and anti-diabetic, anti-oxidative, immune-modulatory, anti-cholesterolemic, or microbiome modulation. This review aims at disc...
http://hdl.handle.net/11019/1678
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Lactic Acid Bacteria and Bifidobacteria with Potential to Design Natural Biofunctional Health-Promoting Dairy Foods
(2018)
Linares, Daniel M.; Gomez, Carolina; Renes, Erica; Fresno, José M.; Tornadijo, María E....
Lactic Acid Bacteria and Bifidobacteria with Potential to Design Natural Biofunctional Health-Promoting Dairy Foods
(2018)
Linares, Daniel M.; Gomez, Carolina; Renes, Erica; Fresno, José M.; Tornadijo, María E.; Ross, R Paul; STANTON, CATHERINE
Abstract:
Consumer interest in healthy lifestyle and health-promoting natural products is a major driving force for the increasing global demand of biofunctional dairy foods. A number of commercial sources sell synthetic formulations of bioactive substances for use as dietary supplements. However, the bioactive-enrichment of health-oriented foods by naturally occurring microorganisms during dairy fermentation is in increased demand. While participating in milk fermentation, lactic acid bacteria can be exploited in situ as microbial sources for naturally enriching dairy products with a broad range of bioactive components that may cover different health aspects. Several of these bioactive metabolites are industrially and economically important, as they are claimed to exert diverse health-promoting activities on the consumer, such as anti-hypertensive, anti-inflammatory, and anti-diabetic, anti-oxidative, immune-modulatory, anti-cholesterolemic, or microbiome modulation. This review aims at disc...
http://hdl.handle.net/11019/1645
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Lactic acid bacteria fermentation of wort as a tool to add functionality in malting, brewing and novel beverages
(2017)
Peyer, Lorenzo
Lactic acid bacteria fermentation of wort as a tool to add functionality in malting, brewing and novel beverages
(2017)
Peyer, Lorenzo
Abstract:
This thesis addresses the investigation of several strains of LAB for biopreservation using in vitro and in situ studies, as well as for acidification of wort to be applied during sour brewing and as novel beverages. The impact of bacterial carboxylic acids, with emphasis on phenolic antifungal compounds, was assessed against Fusarium culmorum, a common fungal spoilage organism in malt. The contribution of phenolic acids to the overall fungal inhibition was found to be limited unless combined with lactate and acetate in a low pH environment, suggesting their contribution in a complex, synergistic mechanism. An antifungal ingredient was produced by fermenting wort with Lactobacillus brevis R2Δ. When this substrate was applied on barley during steeping and germination, it significantly reduced the amount of fusaria infection, detoxified the substrate and increased the extract yields. To enrich even more bacterial metabolites in wort, an extension of acidification could be achieved by ...
http://hdl.handle.net/10468/4694
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Lactobacillus rossiae, a vitamin B12 producer, represents a metabolically versatile species within the genus Lactobacillus
(2014)
De Angelis, Maria; Bottacini, Francesca; Fosso, Bruno; Kelleher, Philip; Calasso, Maria...
Lactobacillus rossiae, a vitamin B12 producer, represents a metabolically versatile species within the genus Lactobacillus
(2014)
De Angelis, Maria; Bottacini, Francesca; Fosso, Bruno; Kelleher, Philip; Calasso, Maria; Di Cagno, Raffaella; Ventura, Marco; Picardi, Ernesto; van Sinderen, Douwe; Gobbetti, Marco
Abstract:
Lactobacillus rossiae is an obligately hetero-fermentative lactic acid bacterium, which can be isolated from a broad range of environments including sourdoughs, vegetables, fermented meat and flour, as well as the gastrointestinal tract of both humans and animals. In order to unravel distinctive genomic features of this particular species and investigate the phylogenetic positioning within the genus Lactobacillus, comparative genomics and phylogenomic approaches, followed by functional analyses were performed on L. rossiae DSM 15814(T), showing how this type strain not only occupies an independent phylogenetic branch, but also possesses genomic features underscoring its biotechnological potential. This strain in fact represents one of a small number of bacteria known to encode a complete de novo biosynthetic pathway of vitamin B-12 (in addition to other B vitamins such as folate and riboflavin). In addition, it possesses the capacity to utilize an extensive set of carbon sources, a ...
http://hdl.handle.net/10468/2323
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Lactococcus lactis is capable of improving the riboflavin status in deficient rats
(2005)
LeBlanc, Jean Guy; Burgess, Catherine M.; Sesma, Fernando; de Giori, Graciela Savoy; va...
Lactococcus lactis is capable of improving the riboflavin status in deficient rats
(2005)
LeBlanc, Jean Guy; Burgess, Catherine M.; Sesma, Fernando; de Giori, Graciela Savoy; van Sinderen, Douwe
Abstract:
Lactococcus lactis is a commonly used starter strain that can be converted from a vitamin B2 consumer into a vitamin B2 'factory' by over-expressing its riboflavin biosynthesis genes. The present study was conducted to assess in a rat bioassay the response of riboflavin produced by GM or native lactic acid bacteria (LAB). The riboflavin-producing strains were able to eliminate most physiological manifestations of ariboflavinosis such as stunted growth, elevated erythrocyte glutathione reductase activation coefficient values and hepatomegalia that were observed using a riboflavin depletion–repletion model. Riboflavin status and growth rates were greatly improved when the depleted rats were fed with cultures of L. lactis that overproduced this vitamin whereas the native strain did not show the same effect. The present study is the first animal trial with food containing living bacteria that were engineered to overproduce riboflavin. These results pave the way for analysing t...
http://hdl.handle.net/10468/843
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Optimization of Lactic Acid Fermentation of York Cabbage for the Development of Potential Probiotic Products
(2012)
Jaiswal, Amit; Gupta, Shilpi; Abu-Ghannam, Nissreen
Optimization of Lactic Acid Fermentation of York Cabbage for the Development of Potential Probiotic Products
(2012)
Jaiswal, Amit; Gupta, Shilpi; Abu-Ghannam, Nissreen
Abstract:
In the present study, York cabbage was used as the sole substrate for the lactic acid (LA) fermentation with Lactobacillus plantarum. York cabbage was blanched at 95°C for 12min to inactivate surface microflora prior to fermentation. To achieve an optimal fermentation condition which would result in higher release of phytochemicals and antioxidant (AO) capacity in the broth, Box-Behnken design integrating a desirability approach was used. A second-order polynomial equation was developed indicating the effect of solute/liquid ratio, agitation rate and fermentation time on desired parameters. The optimized factors were used for fermenting York cabbage to obtain maximum bacterial growth, LA, polyphenols and AO capacity. There was ≈5 log cfu/ml increment in bacterial growth after fermentation, whereas LA production reached up to 4.97mg/ml. Results showed that fermentation retains 95-98% and 90-95% of TPC and AOC, respectively. During refrigerated storage (4°C), the cell numbers, bioacti...
https://arrow.dit.ie/schfsehart/92
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Osmotic stress tolerance mechanisms in Lactococcus lactis
(1998)
O'Callaghan, John
Osmotic stress tolerance mechanisms in Lactococcus lactis
(1998)
O'Callaghan, John
Abstract:
The response of Lactococcus lactis subsp. cremoris NCDO 712 to low water activity (aw) was investigated, both in relation to growth following moderate reductions in the aw and in terms of survival following substantial reduction of the aw with NaCI. Lc.lactis NCDO 712 was capable of growth in the presence of ≤ 4% w/v NaCI and concentrations in excess of 4% w/v were lethal to the cells. The presence of magnesium ions significantly increased the resistance of NCDO 712 to challenge with NaCI and also to challenge with high temperature or low pH. Survival of Lc.lactis NCDO 712 exposed to high NaCI concentrations was growth phase dependent and cells were most sensitive in the early exponential phase of growth. Pre-exposure to 3% w/v NaCI induced limited protection against subsequent challenge with higher NaCI concentrations. The induction was inhibited by chloramphenicol and even when induced, the response did not protect against NaCI concentrations> 10% w/v. When growing at low aw, p...
http://hdl.handle.net/10468/1620
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Oxidation-reduction potential and its influence on Cheddar cheese quality
(2015)
Caldeo, Veronica
Oxidation-reduction potential and its influence on Cheddar cheese quality
(2015)
Caldeo, Veronica
Abstract:
Oxidation-reduction (redox) potential is a fundamental physicochemical parameter that affects the growth of microorganisms in dairy products and contributes to a balanced flavour development in cheese. Even though redox potential has an important impact on the quality of dairy products, it is not usually monitored in dairy industry. The aims of this thesis were to develop practical methods for measuring redox potential in cheese, to provide detailed information on changes in redox potential during the cheesemaking and cheese ripening and how this parameter is influenced by starter systems and to understand the relationship between redox potential and cheese quality. Methods were developed for monitoring redox potential during cheesemaking and early in ripening. Changes in redox potential during laboratory scale manufacture of Cheddar, Gouda, Emmental, and Camembert cheeses were determined. Distinctive kinetics of reduction in redox potential during cheesemakings were observed, and d...
http://hdl.handle.net/10468/2125
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Polyol-producing lactic acid bacteria isolated from sourdough and their application to reduce sugar in a quinoa-based milk substitute
(2018)
Jeske, Stephanie; Zannini, Emanuele; Lynch, Kieran M.; Coffey, Aidan; Arendt, Elke K.
Polyol-producing lactic acid bacteria isolated from sourdough and their application to reduce sugar in a quinoa-based milk substitute
(2018)
Jeske, Stephanie; Zannini, Emanuele; Lynch, Kieran M.; Coffey, Aidan; Arendt, Elke K.
Abstract:
The interest for plant-based dairy substitutes is expanding rapidly and consumers are opting for nutritious and healthy dairy alternatives. The reduction of sugar using different exogenous enzymes in combination with lactic acid fermentation in a quinoa-based milk substitute was explored in this study. Different amylolytic enzymes were used to release sugar from the raw material, which were further metabolised to mannitol, due to fermentation with two heterofermentative lactic acid bacteria. Using these two biotechnological techniques enables the reduction of sugar, while also preserving some of the sweetness. Leuconostoc citreum TR116, and Lactobacillus brevis TR055 were isolated from sourdough. Both strains showed high viable cell counts with L citreum TR116 > 8.4 and L. brevis TR055 > 9.3 log cfu/mL, and a reduction in pH to 3.7 and 3.5 respectively. When fructose was available, mannitol was produced in conjunction with acetic acid in addition to lactic acid. Due to these p...
http://hdl.handle.net/10468/7833
Marked
Mark
Reduction of salt in yeasted wheat bread: impact on bread quality and solutions using sourdough fermented by functional lactic acid bacteria strains
(2016)
Belz, Markus C. E.
Reduction of salt in yeasted wheat bread: impact on bread quality and solutions using sourdough fermented by functional lactic acid bacteria strains
(2016)
Belz, Markus C. E.
Abstract:
The dietary intake of sodium chloride has increased considerably over the last few decades due to changes in the human diet. This higher intake has been linked to a number of diseases including hypertension and other cardiovascular diseases. Numerous international health agencies, as well as the food industry, have now recommended a salt intake level of 5-6 g daily, approximately half of the average current daily intake level. Cereal products, and in particular bread, are a major source of salt in the Western diet. Therefore, any reduction in the level of salt in bread could have a major impact on global health. However, salt is a critical ingredient in bread production, and its reduction can have a deleterious effect on the production process as well as on the final bread quality characteristics such as shelf-life, bread volume and sensory characteristics, all deviating from the bakers’ and consumers’ expectations. This work addresses the feasibility of NaCl reduction in wheat brea...
http://hdl.handle.net/10468/2738
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