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Complete Chloroplast Genome Sequence of a Major Allogamous Forage Species, Perennial Ryegrass (Lolium perenne L.)
Diekmann, Kerstin; Hodkinson, Trevor R.; Wolfe, Kenneth H.; van den Bekerom, Rob Jozef Matheus; Dix, Philip; Barth, Susanne
Lolium perenne L. (perennial ryegrass) is globally one of the most important forage and grassland crops. We sequenced the chloroplast (cp) genome of Lolium perenne cultivar Cashel. The L. perenne cp genome is 135 282 bp with a typical quadripartite structure. It contains genes for 76 unique proteins, 30 tRNAs and four rRNAs. As in other grasses, the genes accD, ycf1 and ycf2 are absent. The genome is of average size within its subfamily Pooideae and of medium size within the Poaceae. Genome size differences are mainly due to length variations in non-coding regions. However, considerable length differences of 1–27 codons in comparison of L. perenne to other Poaceae and 1–68 codons among all Poaceae were also detected. Within the cp genome of this outcrossing cultivar, 10 insertion/deletion polymorphisms and 40 single nucleotide polymorphisms were detected. Two of the polymorphisms involve tiny inversions within hairpin structures. By comparing the genome sequence with RT–PCR products of transcripts for 33 genes, 31 mRNA editing sites were identified, five of them unique to Lolium. The cp genome sequence of L. perenne is available under Accession number AM777385 at the European Molecular Biology Laboratory, National Center for Biotechnology Information and DNA DataBank of Japan.
Keyword(s): Biology; chloroplast genome; Lolium perenne; Poaceae; chloroplast DNA variation; RNA editing
Publication Date:
Type: Journal article
Peer-Reviewed: Yes
Institution: Maynooth University
Funder(s): Teagasc
Citation(s): Diekmann, Kerstin and Hodkinson, Trevor R. and Wolfe, Kenneth H. and van den Bekerom, Rob Jozef Matheus and Dix, Philip and Barth, Susanne (2009) Complete Chloroplast Genome Sequence of a Major Allogamous Forage Species, Perennial Ryegrass (Lolium perenne L.). DNA Research, 16. pp. 165-176. ISSN 1340-2838
Publisher(s): Oxford University Press
File Format(s): other
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First Indexed: 2015-07-17 05:12:38 Last Updated: 2019-02-05 07:08:26