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The effect of mean pore size on cell attachment, proliferation and migration in collagen glycosaminoglycan scaffolds for tissue engineering.
Murphy, Ciara M; Haugh, Matthew G; O'Brien, Fergal J
This article is also available at In the literature there are conflicting reports on the optimal scaffold mean pore size required for successful bone tissue engineering. This study set out to investigate the effect of mean pore size, in a series of collagen-glycosaminoglycan (CG) scaffolds with mean pore sizes ranging from 85 microm to 325 microm, on osteoblast adhesion and early stage proliferation up to 7 days post-seeding. The results show that cell number was highest in scaffolds with the largest pore size of 325 microm. However, an early additional peak in cell number was also seen in scaffolds with a mean pore size of 120 microm at time points up to 48 h post-seeding. This is consistent with previous studies from our laboratory which suggest that scaffold specific surface area plays an important role on initial cell adhesion. This early peak disappears following cell proliferation indicating that while specific surface area may be important for initial cell adhesion, improved cell migration provided by scaffolds with pores above 300 microm overcomes this effect. An added advantage of the larger pores is a reduction in cell aggregations that develop along the edges of the scaffolds. Ultimately scaffolds with a mean pore size of 325 microm were deemed optimal for bone tissue engineering.
Keyword(s): 3T3 Cells; Animals; Biocompatible Materials; Bone and Bones; Cell Adhesion; Cell Movement; Cell Proliferation; Cells; Cultured; Collagen; Glycosaminoglycans; Materials Testing; Mice; Osteoblasts; Porosity; Tissue Engineering; Tissue Scaffolds; Anatomy
Publication Date:
Type: Journal article
Peer-Reviewed: Yes
Institution: Royal College of Surgeons in Ireland
Citation(s): Murphy CM, Haugh MG, O'Brien FJ. The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for tissue engineering. Biomaterials. 2010;31(3):461-6.
Publisher(s): Butterworth-Heinemann In Association With The Biological Engineering Society
File Format(s): application/pdf
Related Link(s):
First Indexed: 2015-07-31 15:34:35 Last Updated: 2018-02-13 07:23:07