Institutions | About Us | Help | Gaeilge
rian logo


Mark
Go Back
A peptide corresponding to the neuropilin-1-binding site on VEGF(165) induces apoptosis of neuropilin-1-expressing breast tumour cells.
Barr, Martin P; Byrne, Anne Marie; Duffy, Angela M; Condron, Claire M; Devocelle, Marc; Harriott, Patrick; Bouchier-Hayes, David J; Harmey, Judith H
<p>The original article is available at <a href="http://www.nature.com">www.nature.com</a></p> <p>There is increasing evidence that vascular endothelial growth factor (VEGF) has autocrine as well as paracrine functions in tumour biology. Vascular endothelial growth factor-mediated cell survival signalling occurs via the classical tyrosine kinase receptors Flt-1, KDR/Flk-1 and the more novel neuropilin (NP) receptors, NP-1 and NP-2. A 24-mer peptide, which binds to neuropilin-1, induced apoptosis of murine and human breast carcinoma cells, whereas a peptide directed against KDR had no effect. Both anti-NP1 and anti-KDR peptides induced endothelial cell apoptosis. Confocal microscopy using 5-(6)-carboxyfluorescein-labelled peptides showed that anti-NP1 bound to both tumour and endothelial cells, whereas anti-KDR bound endothelial cells only. This study demonstrates that NP-1 plays an essential role in autocrine antiapoptotic signalling by VEGF in tumour cells and that NP1-blockade induces tumour cell and endothelial cell apoptosis. Specific peptides can therefore be used to target both autocrine (tumour cells) and paracrine (endothelial cells) signalling by VEGF.</p>
Keyword(s): Adenocarcinoma; Animals; Apoptosis; Blotting; Western; Breast Neoplasms; Cell Line; Tumor; Endothelial Cells; Flow Cytometry; Humans; Mice; Microscopy; Confocal; Neuropilin-1; Peptides; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-2; Medicine and Health Sciences; Surgery
Publication Date:
2005
Type: Journal article
Peer-Reviewed: Yes
Institution: Royal College of Surgeons in Ireland
Citation(s): Barr MP, Byrne AM, Duffy AM, Condron CM, Devocelle M, Harriott P, Bouchier-Hayes DJ, Harmey JH. A peptide corresponding to the neuropilin-1-binding site on VEGF(165) induces apoptosis of neuropilin-1-expressing breast tumour cells. British Journal Cancer. 2005;92(2):328-33.
Publisher(s): Nature Publishing Group
File Format(s): application/pdf
First Indexed: 2015-07-31 15:34:54 Last Updated: 2018-02-13 07:22:35