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Coupling Freshly Isolated CD44(+) Infrapatellar Fat Pad-Derived Stromal Cells with a TGF-β3 Eluting Cartilage ECM-Derived Scaffold as a Single-Stage Strategy for Promoting Chondrogenesis.
Almeida, Henrique V; Cunniffe, Gráinne M; Vinardell, Tatiana; Buckley, Conor T; O'Brien, Fergal; Kelly, Daniel J
<p>This article is also available at <a href="http://onlinelibrary.wiley.com/maintenance/?DOI=10.1002/adhm.201400687">http://onlinelibrary.wiley.com/maintenance/?DOI=10.1002/adhm.201400687</a></p> <p>An alternative strategy to the use of in vitro expanded cells in regenerative medicine is the use of freshly isolated stromal cells, where a bioactive scaffold is used to provide an environment conducive to proliferation and tissue-specific differentiation in vivo. The objective of this study is to develop a cartilage extracellular matrix (ECM)-derived scaffold that could facilitate the rapid proliferation and chondrogenic differentiation of freshly isolated stromal cells. By freeze-drying cryomilled cartilage ECM of differing concentrations, it is possible to produce scaffolds with a range of pore sizes. The migration, proliferation, and chondrogenic differentiation of infrapatellar fat pad-derived stem cells (FPSCs) depend on the concentration/porosity of these scaffolds, with greater sulphated glycosaminoglycan (sGAG) accumulation observed in scaffolds with larger-sized pores. It is then sought to determine if freshly isolated fat pad-derived stromal cells, seeded onto a transforming growth factor (TGF)-β3 eluting ECM-derived scaffold, could promote chondrogenesis in vivo. While a more cartilage-like tissue could be generated using culture expanded FPSCs compared to nonenriched freshly isolated cells, fresh CD44(+) stromal cells are capable of producing a tissue in vivo that stained strongly for sGAGs and type II collagen. These findings open up new possibilities for in-theatre cell-based therapies for joint regeneration.</p>
Keyword(s): Articular Cartilage; Extracellular Matrix; Single-Stage Therapy; Scaffold; Anatomy
Publication Date:
2015
Type: Journal article
Peer-Reviewed: No
Institution: Royal College of Surgeons in Ireland
Citation(s): Almeida HV, Cunniffe GM, Vinardell T, Buckley CT, O'Brien FJ, Kelly DJ. Coupling Freshly Isolated CD44(+) Infrapatellar Fat Pad-Derived Stromal Cells with a TGF-β3 Eluting Cartilage ECM-Derived Scaffold as a Single-Stage Strategy for Promoting Chondrogenesis. Advanced Healthcare Materials. 2015;4(7):1043-53.
Publisher(s): John Wiley & Sons, Inc.
File Format(s): application/pdf
Related Link(s): http://epubs.rcsi.ie/anat/74/
First Indexed: 2015-09-16 06:15:02 Last Updated: 2018-02-13 07:18:42