Institutions | About Us | Help | Gaeilge
rian logo


Mark
Go Back
Development of a microfluidic device and imaging system for the monitoring of anti-platelet therapy
McCluskey, Peter M.
This work details the development of two distinct prototype point-of-care diagnostic systems to be used to determine the ecacy of anti-platelet ther- apies. Novel micro uidic chips were designed for each system, to enable the on-chip testing of multiple anti-platelet therapies. The prototypes work with whole blood and integrate with a new detection method used to de- termine the percentage of platelets adhered to an array of brinogen spots. The system uses customised image analysis software to objectively quantify single platelet binding events from a single blood sample, without the need to add any form of labelling to the blood. The system was validated by comparing the percentage of platelets adhered from `normal' blood samples compared to samples treated with an established platelet therapy. These treatments inhibit a specic receptor on the platelet surface, thus inter- fering with stable adhesion to brinogen, rendering platelets \less sticky". This prototype point-of-care device could allow for the automated testing of large numbers of patient samples, potentially diagnosing individuals at risk of suering a cardiovascular event or undiagnosed bleeding disorder.
Keyword(s): Biomedical engineering; Platelets; MEMS
Publication Date:
2015
Type: Doctoral thesis
Peer-Reviewed: No
Language(s): English
Institution: Dublin City University
Funder(s): Science Foundation Ireland
Citation(s): McCluskey, Peter M. (2015) Development of a microfluidic device and imaging system for the monitoring of anti-platelet therapy. PhD thesis, Dublin City University.
Publisher(s): Dublin City University. School of Mechanical and Manufacturing Engineering
File Format(s): application/pdf
Supervisor(s): Corcoran, Brian
Kent, Nigel
Related Link(s): http://doras.dcu.ie/20791/1/PhD_peter_McCluskey.pdf
First Indexed: 2015-11-26 05:05:11 Last Updated: 2018-05-15 06:07:27