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Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds.
Raftery, Rosanne M; Tierney, Erica G; Curtin, Caroline M; Cryan, Sally-Ann; O'Brien, Fergal
<p>This article is also available at <a href="http://ac.els-cdn.com/S016836591500303X/1-s2.0-S016836591500303X-main.pdf?_tid=c7dc6fd6-cff4-11e5-a499-00000aab0f01&acdnat=1455108773_e5d608115e63a4787b4221e5f3a8bec8">http://ac.els-cdn.com/S016836591500303X/1-s2.0-S016836591500303X-main.pdf?_tid=c7dc6fd6-cff4-11e5-a499-00000aab0f01&acdnat=1455108773_e5d608115e63a4787b4221e5f3a8bec8</a></p> <p>Biomaterial scaffolds that support cell infiltration and tissue formation can also function as platforms for the delivery of therapeutics such as drugs, proteins, and genes. As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications. The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs). A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects. When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28days and interestingly the composition of the scaffold had an effect on transfection efficiency. These results demonstrate that by simply varying the scaffold composition and the gene (or combinations thereof) chosen; the system has potential for a myriad of therapeutic applications.</p>
Keyword(s): Chitosan Nanoparticles; Gene Delivery; Mesenchymal Stem Cells (MSCs); Gene-Activated Scaffold; Tissue Engineering; Anatomy
Publication Date:
2015
Type: Journal article
Peer-Reviewed: Yes
Institution: Royal College of Surgeons in Ireland
Citation(s): Rafferty RM, Tierney EG, Curtin CM, Cryan SA, O'Brien FJ. Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds. Journal of Control Release. 2015;210:84-94.
Publisher(s): Elsevier Science Publishers
File Format(s): application/pdf
Related Link(s): http://epubs.rcsi.ie/anatart/87/
First Indexed: 2016-02-11 06:15:02 Last Updated: 2018-02-13 07:18:08