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Evaluation of cartilage repair by mesenchymal stem cells seeded on a PEOT/PBT scaffold in an osteochondral defect
Barron, Valerie; Merghani, Khalid; Shaw, Georgina; Coleman, Cynthia; Hayes, Jessica; Ansboro, Sharon; Manian, Abi; O'Malley, Grace; Connolly, Emma; Nandakumar, Ananda; Van Blitterswijk, Clemen; Habibovic, Pamela; Moroni, Lorenzo; Shannon, Fintan; Barry, Frank; Murphy, Mary
The main objective of this study was to evaluate the effectiveness of a mesenchymal stem cell (MSC)-seeded polyethylene-oxide-terephthalate/polybutylene-terephthalate (PEOT/PBT) scaffold for cartilage tissue repair in an osteochondral defect using a rabbit model. Material characterisation using scanning electron microscopy indicated that the scaffold had a 3D architecture characteristic of the additive manufacturing fabrication method, with a strut diameter of 296 +/- A 52 mu m and a pore size of 512 +/- A 22 mu m x 476 +/- A 25 mu m x 180 +/- A 30 mu m. In vitro optimisation revealed that the scaffold did not generate an adverse cell response, optimal cell loading conditions were achieved using 50 mu g/ml fibronectin and a cell seeding density of 25 x 10(6) cells/ml and glycosaminoglycan (GAG) accumulation after 28 days culture in the presence of TGF beta 3 indicated positive chondrogenesis. Cell-seeded scaffolds were implanted in osteochondral defects for 12 weeks, with cell-free scaffolds and empty defects employed as controls. On examination of toluidine blue staining for chondrogenesis and GAG accumulation, both the empty defect and the cell-seeded scaffold appeared to promote repair. However, the empty defect and the cell-free scaffold stained positive for collagen type I or fibrocartilage, while the cell-seeded scaffold stained positive for collagen type II indicative of hyaline cartilage and was statistically better than the cell-free scaffold in the blinded histological evaluation. In summary, MSCs in combination with a 3D PEOT/PBT scaffold created a reparative environment for cartilage repair. Science Foundation Ireland (SFI) Strategic Research Cluster (SRC), Grant No. SFI: 09/SRC B1794, Wellcome Trust Biomedical Vacation Scholarships Grant Number WTD004448, the European Union’s 7th Framework Programme under Grant Agreement No. HEALTH-2007-B- 223298 (PurStem)
Keyword(s): Additive manufacturing; 3D scaffold; PEOT/PBT; Mesenchymal stem cells; Cartilage repair; Dynamic mechanical properties; Animal models; Articular cartilage; Tissue; RGD; Differentiation; Regeneration; Society
Publication Date:
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Contributor(s): |~|
Institution: NUI Galway
Publisher(s): Springer Verlag
File Format(s): application/pdf
First Indexed: 2016-06-10 09:34:19 Last Updated: 2019-09-20 06:34:11