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Direct gene transfer in potato: A comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts
Craig, W.; Gargano, Daniela; Scotti, Nunzia; Nguyen, T.T.; Lao, N.T.; Kavanagh, T.A.; Dix, Philip; Cardi, T.
Direct gene transfer methods in potato would facilitate the transfer of multiple genes and the manipulation of metabolic pathways in this species. In this study, up to 1.8 transformation events per shot (=0.5 per bombarded leaf) and 67.2 events per million protoplasts treated were obtained with particle bombardment and PEG-mediated direct DNA uptake, respectively. Limited disassociation of both HPT and GUS genes appeared to occur during the process of integration in only 19% of transformants. A large number of transformed potato plants with transgene expression at levels comparable to Agrobacterium-mediated transformation was obtained. High levels of GUS expression were only obtained in lines derived from PEG treatment. No correlation between the number of gene insertions and gene expression levels was found, suggesting that multiple insertions may have little or no effect on transgene expression.
Keyword(s): Direct gene transfer; Nuclear transformation; Particle bombardment; Potato; Protoplasts
Publication Date:
2005
Type: Journal article
Peer-Reviewed: Yes
Institution: Maynooth University
Citation(s): Craig, W. and Gargano, Daniela and Scotti, Nunzia and Nguyen, T.T. and Lao, N.T. and Kavanagh, T.A. and Dix, Philip and Cardi, T. (2005) Direct gene transfer in potato: A comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts. Plant Cell Reports, 10. pp. 603-611. ISSN 0721-7714
Publisher(s): Springer
File Format(s): other
Related Link(s): http://eprints.maynoothuniversity.ie/7363/1/PD_direct%202005.pdf
First Indexed: 2016-08-25 05:00:45 Last Updated: 2018-09-04 06:11:45