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Evaluation of the early in vivo response of a functionally graded macroporous scaffold in an osteochondral defect in a rabbit model
Barron, Valerie; Neary, Martin; Mohamed, Khalid Merghani Salid; Ansboro, Sharon; Shaw, Georgina; O'Malley, Grace; Rooney, Niall; Barry, Frank; Murphy, Mary
Cartilage tissue engineering is a multifactorial problem requiring a wide range of material property requirements from provision of biological cues to facilitation of mechanical support in load-bearing diarthrodial joints. The study aim was to design, fabricate and characterize a template to promote endogenous cell recruitment for enhanced cartilage repair. A polylactic acid poly-epsilon-caprolactone (PLCL) support structure was fabricated using laser micromachining technology and thermal crimping to create a functionally-graded open pore network scaffold with a compressive modulus of 9.98 +/- A 1.41 MPa and a compressive stress at 50% strain of 8.59 +/- A 1.35 MPa. In parallel, rabbit mesenchymal stem cells were isolated and their growth characteristics, morphology and multipotency confirmed. Sterilization had no effect on construct chemical structure and cellular compatibility was confirmed. After four weeks implantation in an osteochondral defect in a rabbit model to assess biocompatibility, there was no evidence of inflammation or giant cells. Moreover, acellular constructs performed better than cell-seeded constructs with endogenous progenitor cells homing through microtunnels, differentiating to form neo-cartilage and strengthening integration with native tissue. These results suggest, albeit at an early stage of repair, that by modulating the architecture of a macroporous scaffold, pre-seeding with MSCs is not necessary for hyaline cartilage repair. European Union’s 7th Framework Programme under Grant Agreement No. HEALTH-2007-B-223298 (PurStem), Science Foundation Ireland (Grant Number 09/SRC/B1794), Wellcome Trust Biomedical Vacation Scholarships Grant Number WTD004448 and the Irish Government’s Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007–2013 2016-10-05
Keyword(s): Functionally graded; Polylactic acid-e-polycaprolactone; Cartilage repair; Integration; Neotissue formation; Mesenchymal stem cells; cart; Articular cartilage; Mechanical properties; Tissue formation; Regeneration; Therapy; Carrier; Chondrogenesis; Regenerative medicine
Publication Date:
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Contributor(s): |~|
Institution: NUI Galway
Publisher(s): Springer Verlag
File Format(s): application/pdf
First Indexed: 2016-10-06 09:34:55 Last Updated: 2019-09-20 06:34:11