|
?-adrenoceptor-mediated regulation of matrix metalloproteinase-9 in fibroblasts |
Rietz, Anne
|
|
|
|
THESIS 9260 Adrenoceptor signalling in fibroblasts: Intracellular cAMP levels were measured by a direct cAMP enzyme immunoassay. Isoprenaline (ISO, 1 ?M) increased intracellular cAMP concentration by 1.3 and 4.8 fold, P<0.05, after 10 min in the NIH3T3 fibroblasts and human cardiac ventricular fibroblasts (HCVF) respectively. These effects were abolished by pre-treatment with propranolol (PROP, 10 ?M; P<0.05). Nuclear translocation of phospho-CREB was increased by isoprenaline (0.1 ?M) and phenylephrine (10 ?M) increased in NIH3T3 fibroblasts (P<0.05). Western blot analysis indicated that isoprenaline (1 ?M) and phenylephrine (10 ?M) caused a biphasic increase in p38 MAPK and a sustained increase in ERK1/2 MAPK activation over a 30 min period in NIH3T3 fibroblasts. However, in HCVF cells, isoprenaline (1 ?M) decreased ERKl/2 MAPK phosphorylation by 70% after 30 min, an effect abolished by propranolol (10 ?M; ISO vs. ISO/PROP: 0.30?0.06 vs. 1.05?0.10 AU; P<0.05).
|
|
Keyword(s):
|
Pharmacology and Therapeutics, Ph.D.; Ph.D. Trinity College Dublin |
Publication Date:
|
2011 |
|
Type:
|
Doctoral thesis |
|
Peer-Reviewed:
|
Unknown |
|
Language(s):
|
English |
|
Institution:
|
Trinity College Dublin |
|
Citation(s):
|
Anne Rietz, '?-adrenoceptor-mediated regulation of matrix metalloproteinase-9 in fibroblasts', [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Pharmacology & Therapeutics, 2011, pp 256 |
|
Publisher(s):
|
Trinity College (Dublin, Ireland). School of Medicine. Discipline of Pharmacology & Therapeutics |
|
Supervisor(s):
|
Spiers, Paul |
|
First Indexed:
2017-02-13 06:59:28 Last Updated:
2017-07-05 05:28:01 |
