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?-adrenoceptor-mediated regulation of matrix metalloproteinase-9 in fibroblasts
Rietz, Anne
THESIS 9260 Adrenoceptor signalling in fibroblasts: Intracellular cAMP levels were measured by a direct cAMP enzyme immunoassay. Isoprenaline (ISO, 1 ?M) increased intracellular cAMP concentration by 1.3 and 4.8 fold, P<0.05, after 10 min in the NIH3T3 fibroblasts and human cardiac ventricular fibroblasts (HCVF) respectively. These effects were abolished by pre-treatment with propranolol (PROP, 10 ?M; P<0.05). Nuclear translocation of phospho-CREB was increased by isoprenaline (0.1 ?M) and phenylephrine (10 ?M) increased in NIH3T3 fibroblasts (P<0.05). Western blot analysis indicated that isoprenaline (1 ?M) and phenylephrine (10 ?M) caused a biphasic increase in p38 MAPK and a sustained increase in ERK1/2 MAPK activation over a 30 min period in NIH3T3 fibroblasts. However, in HCVF cells, isoprenaline (1 ?M) decreased ERKl/2 MAPK phosphorylation by 70% after 30 min, an effect abolished by propranolol (10 ?M; ISO vs. ISO/PROP: 0.30?0.06 vs. 1.05?0.10 AU; P<0.05).
Keyword(s): Pharmacology and Therapeutics, Ph.D.; Ph.D. Trinity College Dublin
Publication Date:
2011
Type: Doctoral thesis
Peer-Reviewed: Unknown
Language(s): English
Institution: Trinity College Dublin
Citation(s): Anne Rietz, '?-adrenoceptor-mediated regulation of matrix metalloproteinase-9 in fibroblasts', [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Pharmacology & Therapeutics, 2011, pp 256
Publisher(s): Trinity College (Dublin, Ireland). School of Medicine. Discipline of Pharmacology & Therapeutics
Supervisor(s): Spiers, Paul
First Indexed: 2017-02-13 06:59:28 Last Updated: 2018-08-24 06:53:31