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Milk protein-derived peptides induce 5-HT2C-mediated satiety,in vivo
Schellekens, Harriët; Nongonierma, Alice B.; Clarke, Gerard; van Oeffelen, Wesley E.P.A.; Fitzgerald, Richard J.; Dinan, Timothy G.; Cryan, John F.
This study investigates the ability of milk protein-derived peptides to specifically activate the serotonin 2C (5-HT2C) receptor, a key receptor in central regulation of food intake. A dose dependent 5-HT2C receptor activation by the 1 kDa ultrafiltration permeates of a sodium caseinate (NaCNH-1 kDa permeate) and a whey protein hydrolysate (WPH-1 kDa permeate) was demonstrated using an intracellular calcium mobilization assay in human embryonic kidney (Hek) cells expressing the 5-HT2C receptor. Both samples activated the 5-HT2C but not the 5-HT2A and 5-HT2B receptors. NaCNH-1 kDa permeate significantly (p < 0.01) reduced cumulative food intake when administered to male mice (C57Bl/6) by intraperitoneal injection at 500 mg kg-1 body weight. In contrast, no effect of WPH-1 kDa permeate could be seen on food intake in vivo. These results demonstrate the promising appetite-suppressing potential of NaCN-derived peptides, targeting the 5-HT2C receptor. ACCEPTED peer-reviewed
Keyword(s): 5-HT2C serotonin receptors; amino-acids profiles; food intake; alpha-lactalbumin
Publication Date:
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Institution: University of Limerick
Funder(s): Enterprise Ireland
Citation(s): International Dairy Journal;38, 1, pp. 55-64
Publisher(s): Elsevier
First Indexed: 2017-04-15 06:31:46 Last Updated: 2018-08-09 06:42:01