Institutions | About Us | Help | Gaeilge
rian logo


Mark
Go Back
Quantification of fluid shear stress in bone tissue engineering scaffolds with spherical and cubical pore architectures
Zhao, Feihu; Vaughan, Ted J.; McNamara, Laoise M.
Recent studies have shown that mechanical stimulation, in the form of fluid perfusion and mechanical compression, can enhance osteogenic differentiation of mesenchymal stem cells and bone cells within tissue engineering scaffolds in vitro. The precise nature of mechanical stimulation within tissue engineering scaffolds is not only dictated by the exogenously applied loading regime, but also depends on the geometric features of the scaffold, in particular architecture, pore size and porosity. However, the precise contribution of each geometric feature towards the resulting mechanical stimulation within a scaffold is difficult to characterise due to the wide range of interacting parameters. In this study, we have applied a fluid-structure interaction model to investigate the role of scaffold geometry (architecture, pore size and porosity) on pore wall shear stress (WSS) under a range of different loading scenarios: fluid perfusion, mechanical compression and a combination of perfusion and compression. It is found that scaffold geometry (spherical and cubical pores), in particular the pore size, has a significant influence on the stimulation within scaffolds. Furthermore, we observed an amplified WSS within scaffolds under a combination of fluid perfusion and mechanical compression, which exceeded that caused by individual fluid perfusion or mechanical compression approximately threefold. By conducting this comprehensive parametric variation study, an expression was generated to allow the design and optimisation of 3D TE scaffolds and inform experimental loading regimes so that a desired level of mechanical stimulation, in terms of WSS is generated within the scaffold. The authors would like to acknowledge the research grant from European Research Council (ERC) under grant number 258992 (BONEMECHBIO). 2017-06-01
Keyword(s): Bone tissue-engineered scaffold; Scaffold geometry; Mechanical stimulation; Wall shear stress; Computational modelling; Biomedical engineering; Collagen glycosaminoglycan scaffolds; Stem cell differentiation; Marrow stromal cells; Osteogenic differentiation; 3 dimensional scaffolds; Perfusion bioreactor; Substrate stiffness; Porous scaffold; Flow perfusion
Publication Date:
2016
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Contributor(s): |~|
Institution: NUI Galway
Publisher(s): Springer Verlag
File Format(s): application/pdf
First Indexed: 2017-07-07 05:30:08 Last Updated: 2017-07-07 05:30:08