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Coiled coil type neoglycoproteins presenting three lactose residues
Sweeney, Sinclair M.; Bullen, Gemma A.; Gillis, Richard B.; Adams, Gary G.; Rowe, Arthur J.; Harding, Stephen E.; Tucker, James H.R.; Peacock, Anna F.A.; Murphy, Paul V.
Scaffold design, synthesis and application are relevant for biomedical research. For example, multivalent interactions, such as those between cell surface glycoproteins and lectins can influence the potency and duration of signalling. The spacing between carbohydrates on their native protein scaffold could be important. Herein, the coiled coil design principle is used to generate synthetic coiled coil type glycoproteins, where three lactose residues are grafted to the coil via N-linkages to asparagine. Molecular modelling indicates that the distance between the galactose anomeric carbon atoms on the neoglycoproteins is similar to 30 angstrom. The inclusion of lactose was accommodated in both the final heptad towards the N-terminus, or more centrally in the penultimate heptad. In either case, neither the helicity nor the assembly to the trimeric form was unduly altered by the presence of the disaccharide. (C) 2016 Elsevier Ltd. All rights reserved. The material described herein was funded by the Irish Research Council (scholarship to S.S.), COST CM1102 (short term scientific mission for S.S. to Birmingham) and the University of Birmingham and EPSRC (studentships for G.A.B.) 2018-02-06
Keyword(s): Scaffold; Glycosylated; Coiled coil; Glycocluster; Building blocks; Lectin binding; Sugar code; Peptides; Design; Glycopeptides; Glycoclusters; Coordination; Scaffolds; Proteins; Chemistry
Publication Date:
2016
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Contributor(s): |~|
Institution: NUI Galway
Publisher(s): Elsevier
File Format(s): application/pdf
First Indexed: 2018-02-09 06:31:09 Last Updated: 2018-02-09 06:31:09