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Cartilage repair in a rabbit model: Development of a novel subchondral defect and assessment of early cartilage repair using a rabbit mesenchymal stem cell seeded scaffold
Neary, Martin
Purpose: To develop a subchondral defect in a rabbit model and assess cartilage repair using a mesenchymal stem cell-seeded scaffold. Materials and Methods: To achieve this goal, a bovine explant model and two pilot rabbit studies were conducted. Initial work using chondral explant tissue harvested from stifle joints of 12-month-old cattle sourced from a local abattoir, followed by a pilot rabbit study demonstrated that a chondral defect was an unsuitable model for the scaffold being studied. Assessment of a novel reproducible technique for subchondral defect creation was conducted using a rabbit model in accordance with University guidelines and ethical approval with 4 test groups, empty 3mm x1mm defect (n=6), empty 3mm x3mm defect (n=6), press-fit scaffold in 3mm x1mm defect (n=6), press-fit cell-seeded scaffold in 3mm x1mm defect (n=6). l. In brief, skeletally mature rabbits, weighing more than 3kg were anesthetized and bilateral defects were created on the medial femoral condyle using an electrical drill with a previously sterilized 2.8mm drill bit covered with a sterile depth stop. The walls of the defect were finished with a dental curette, and the scaffold press-fit into place. Defects was assessed histologically after 28 days implantation. Results: The subchondral defects could be created with the same reproducibility as osteochondral defects and tissue repair was achieved in both cell free and cell-seeded scaffolds. Conclusion: A reproducible subchondral defect was successfully created in a rabbit model to assess cartilage repair using a cell-seeded scaffold. Early cartilage repair was achieved in both cell free and cell-seeded scaffolds, with improved repair seen in the cell free scaffold group
Keyword(s): Cartilage Repair; Stem cell; Chondrogenesis; Scaffold; Chondral defect; Rabbit model; Subchondral defect; Arthritis; Orthopaedics; Medicine; Surgery
Publication Date:
2018
Type: Doctoral thesis
Peer-Reviewed: Unknown
Institution: NUI Galway
Supervisor(s): Murphy, Mary
Shannon, Fintan
Science Foundation Ireland
European Union’s 7th Framework Programme
Wellcome Trust Biomedical Vacation Scholarships
First Indexed: 2018-02-17 06:20:03 Last Updated: 2019-09-20 07:08:00