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miR-222 isoforms are differentially regulated by type-I interferon.
Nejad, Charlotte; Pillman, Katherine A; Siddle, Katherine J; Pépin, Geneviève; Änkö, Minna-Liisa; McCoy, Claire E; Beilharz, Traude H; Quintana-Murci, Lluís; Goodall, Gregory J; Bracken, Cameron P; Gantier, Michael P
<p>This article is also available at http://rnajournal.cshlp.org</p> <p>Endogenous microRNAs (miRNAs) often exist as multiple isoforms (known as "isomiRs") with predominant variation around their 3'-end. Increasing evidence suggests that different isomiRs of the same family can have diverse functional roles, as recently demonstrated with the example of miR-222-3p 3'-end variants. While isomiR levels from a same miRNA family can vary between tissues and cell types, change of templated isomiR stoichiometry to stimulation has not been reported to date. Relying on small RNA-sequencing analyses, we demonstrate here that miR-222-3p 3'-end variants >23 nt are specifically decreased upon interferon (IFN) β stimulation of human fibroblasts, while shorter isoforms are spared. This length-dependent dynamic regulation of long miR-222-3p 3'-isoforms and >40 other miRNA families was confirmed in human monocyte-derived dendritic cells following infection with</p>
Keyword(s): Taqman miRNA assays; interferon; isomiR; microRNA isoform; stem–loop RT-qPCR; Life Sciences
Publication Date:
2018
Type: Journal article
Peer-Reviewed: Yes
Institution: Royal College of Surgeons in Ireland
Citation(s): Nejad C, Pillman KA, Siddle KJ, Pepin G. Anko ML, McCoy CE, Beilharz TH, Quintana-Murci L, Goodall GJ, Bracken CP, Gantier MP. miR-222 isoforms are differentially regulated by type-I interferon. RNA. 2018;24(3):332-341
Publisher(s): Cold Spring Harbor Laboratory Press
File Format(s): application/pdf
Related Link(s): https://epubs.rcsi.ie/mctart/104/
First Indexed: 2018-03-26 07:16:13 Last Updated: 2018-03-26 07:16:13