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Subcellular imaging of liquid silicone coated-intestinal epithelial cells
Nirmalraj, Peter; Lehner, Roman; Thompson, Damien; Rothen-Rutishauser, Barbara; Mayer, Michael
Surface contamination and the formation of water bridge at the nanoscopic contact between an atomic force microscope tip and cell surface limits the maximum achievable spatial resolution on cells under ambient conditions. Structural information from fixed intestinal epithelial cell membrane is enhanced by fabricating a silicone liquid membrane that prevents ambient contaminants and accumulation of water at the interface between the cell membrane and the tip of an atomic force microscope. The clean and stable experimental platform permits the visualisation of the structure and orientation of microvilli present at the apical cell membrane under standard laboratory conditions together with registering topographical features within a microvillus. The method developed here can be implemented for preserving and imaging contaminant-free morphology of fixed cells which is central for both fundamental studies in cell biology and in the emerging field of digital pathology.
Keyword(s): surface contamination; cell surface; spatial resolution; silicone liquid
Publication Date:
2018
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Institution: University of Limerick
Citation(s): Scientific Reports ;8 :10763
Publisher(s): Nature Publishing Group
First Indexed: 2018-08-01 06:27:40 Last Updated: 2018-10-11 06:44:36