Institutions | About Us | Help | Gaeilge
rian logo

Go Back
Perspectives for the microbial production of methyl propionate integrated with product recovery
Pereira, Joana P.C.; van der Wielen, Luuk A.M.; Straathof, Adrie J.J.
A new approach was studied for bio-based production of methyl propionate, a precursor of methyl methacrylate. Recombinant E. coli cells were used to perform a cascade reaction in which 2-butanol is reduced to butanone using alcohol dehydrogenase, and butanone is oxidized to methyl propionate and ethyl acetate using a Baeyer- Villiger monooxygenase (BVMO). Product was removed by in situ stripping. The conversion was in line with a model comprising product formation and stripping kinetics. The maximum conversion rates were 1.14 g-butanone/( L h), 0.11 g-ethyl acetate/(L h), and 0.09 g-methyl propionate/(L h). The enzyme regioselectivity towards methyl propionate was 43% of total ester. Starting from biomass-based production of 2-butanol, full-scale ester production with conventional product purification was calculated to be competitive with petrochemical production if the monooxygenase activity and regioselectivity are enhanced, and the costs of bio-based 2-butanol are minimized.
Keyword(s): biomaterials; bioprocess engineering; integrated product recovery; modeling
Publication Date:
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Institution: University of Limerick
Citation(s): Bioresouce Technology;256, pp. 187-194
Publisher(s): Elsevier
First Indexed: 2018-12-08 06:25:42 Last Updated: 2018-12-08 06:25:42