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Stable Poly(methacrylic acid) Brush Decorated Silica Nano-Particles by ARGET ATRP for Bioconjugation
Iacono, Marcello; Heise, Andreas
<p>The original article is available at <a href="http://www.mdpi.com/2073-4360/7/8/1427">http://www.mdpi.com</a></p> <p>The synthesis of polymer brush decorated silica nano-particles is demonstrated by activator regeneration by electron transfer atom transfer radical polymerization (ARGET ATRP) grafting of poly(tert-butyl methacrylate). ATRP initiator decorated silica nano-particles were obtained using a novel trimethylsiloxane derivatised ATRP initiator obtained by click chemistry. Comparison of de-grafted polymers with polymer obtained from a sacrificial initiator demonstrated good agreement up to 55% monomer conversion. Subsequent mild deprotection of the tert-butyl ester groups using phosphoric acid yielded highly colloidal and pH stable hydrophilic nano-particles comprising approximately 50% methacrylic acid groups. The successful bio-conjugation was achieved by immobilization of Horseradish Peroxidase to the polymer brush decorated nano-particles and the enzyme activity demonstrated in a conversion of o-phenylene diamine dihydrochloride assay.</p>
Keyword(s): Silica Nano-Particles; Polymer Brushes; ARGET ATRP; Poly(methacrylic acid); Bio-Conjugation; Enzyme Immobilization; Chemistry; Medicinal-Pharmaceutical Chemistry; Medicine and Health Sciences
Publication Date:
2015
Type: Journal article
Peer-Reviewed: Yes
Institution: Royal College of Surgeons in Ireland
Citation(s): Iacono M, Heise A. Stable Poly(methacrylic acid) Brush Decorated Silica Nano-Particles by ARGET ATRP for Bioconjugation. Polymers. 2015;7:1427-1443.
Publisher(s): MDPI
File Format(s): application/pdf
Related Link(s): https://epubs.rcsi.ie/pmcart/28
First Indexed: 2019-01-05 07:29:56 Last Updated: 2019-01-05 07:29:56