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Thin multicomponent films for functional enzyme devices and bioreactor particles
Rusling, James F.; Wasalathanthri, Dhanuka P.; Schenkman, John B.
Complex functional films containing enzymes and other biomolecules are easily fabricated in nm-scale thicknesses by using layer-by-layer (LbL) methodologies first popularized by Lvov and Decher. In this review, we highlight the high level functional capabilities possible with LbL films of biomolecules based on our own research experiences. We first describe the basics of enzyme film fabrication by LbL alternate electrostatic adsorption, then discuss how to make functional enzyme-polyion films of remarkably high stability. Focusing on cytochrome P450s, we discuss films developed to electrochemically activate the natural catalytic cycle of these key metabolic enzymes. We then describe multifunctional, multicomponent DNA/enzyme/polyion films on arrays and particle surfaces for high throughput metabolic toxicity screening using electrochemiluminescence and LC-MS/MS. Using multicomponent LbL films, complex functionality for bioanalytical and biochemical purposes can be achieved that is difficult or impossible using conventional approaches.
Keyword(s): layer-by-layer; quartz-crystal microbalance; ultrathin multilayer films; self-assembly process; polyion films; human cytochrome-p450; biocolloid reactors; toxicity assessment; charged surfaces; drug discovery
Publication Date:
2018
Type: Journal article
Peer-Reviewed: Unknown
Institution: NUI Galway
Publisher(s): Royal Society of Chemistry (RSC)
First Indexed: 2019-03-23 06:20:09 Last Updated: 2019-03-23 06:20:09