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Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens
Collins, E.; Glennon, M.; Hanley, S.; Murray, A.-M.; Cormican, M.; Smith, T.; Maher, M.
DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.
Keyword(s): linked immunosorbent-assay; fecal samples; pcr; jejuni; coli
Publication Date:
2018
Type: Journal article
Peer-Reviewed: Unknown
Institution: NUI Galway
Publisher(s): American Society for Microbiology
First Indexed: 2019-03-23 06:55:35 Last Updated: 2019-03-23 06:55:35