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Screening of exosomal microRNAs from colorectal cancer cells
Clancy, Cillian; Khan, Sonja; Glynn, Claire L.; Holian, Emma; Dockery, Peter; Lalor, Pierce; Brown, James A.L.; Joyce, Myles R.; Kerin, Michael J.; Dwyer, Róisín M.
BACKGROUND: Cells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown.OBJECTIVE: To identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRNAs.METHODS: Exosomes were isolated from HCT-116 and HT-29 cell lines. RNA was extracted from exosomes and microRNA array performed. Cells were engineered to express miR-379 (HCT-116-379) or a non-targeting control (HCT-116-NTC) and functional effects were determined. Exosomes secreted by engineered cells were transferred to recipient cells and the impact examined.RESULTS: Microvesicles 40-100 nm in size secreted by cell lines were visualised and confirmed to express exosomal protein CD63. HT-29 exosomes contained 409 miRNAs, HCT-116 exosomes contained 393, and 338 were common to exosomes from both cell lines. Selected targets were validated. HCT-116-379 cells showed decreased proliferation (12-15% decrease, p Funding support was received from Breast Cancer Research and the Irish Cancer Society Collaborative Cancer Research Centre BREAST-PREDICT Grant CCRC13GAL.
Keyword(s): Exosomes; microRNAs; colorectal cancer; BREAST-CANCER; MEDIATED TRANSFER; MESSENGER-RNAS; EXPRESSION; INVASION; MIR-378
Publication Date:
2019
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Contributor(s): Breast Cancer Research; Irish Cancer Society
Institution: NUI Galway
Publisher(s): IOS Press
File Format(s): application/pdf
First Indexed: 2019-09-20 06:39:11 Last Updated: 2019-09-20 06:39:11