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Impact of trichloroethylene exposure on the microbial diversity and protein expression in anaerobic granular biomass at 37°C and 15°C
Siggins, Alma; Enright, Anne-Marie; Abram, Florence; Botting, Catherine; O'Flaherty, Vincent
Granular biomass from a laboratory-scale anaerobic bioreactor trial was analysed to identify changes in microbial community structure and function in response to temperature and trichloroethylene (TCE). Two bioreactors were operated at 37 degrees C, while two were operated at 15 degrees C. At the time of sampling, one of each temperature pair of bioreactors was exposed to process failure-inducing concentrations of TCE (60 mg L-1) while the other served as a TCE-free control. Bacterial community structure was investigated using denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone library analysis. Temperature was identified as an important factor for bacterial community composition, while minor differences were associated with trichloroethylene supplementation. Proteobacteria was the dominant phylum in all bioreactors, while clone library analysis revealed a higher proportion of Bacteroidetes-, Chloroflexi-, and Firmicutes-like clones at 15 degrees C than at 37 degrees C. Comparative metaproteomics in the presence and absence of TCE was carried out by two-dimensional gel electrophoresis (2-DGE), and 28 protein spots were identified, with putative functions related to cellular processes, including methanogenesis, glycolysis, the glyoxylate cycle, and the methyl malonyl pathway. A good agreement between metaproteomic species assignment and phylogenetic information was observed, with 10 of the identified proteins associated with members of the phylum Proteobacteria. This work was supported by Enterprise Ireland, The Irish Environmental Protection Agency, and Science Foundation Ireland.
Keyword(s): WASTE-WATER TREATMENT; 16S RIBOSOMAL-RNA; DEGREES-C; METHANOGENIC ACTIVITY; SLUDGE; COMMUNITY; DIGESTION; PROPIONATE; BACTERIUM; ARCHAEA
Publication Date:
2019
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Contributor(s): Enterprise Ireland; Science Foundation Ireland; Environmental Protection Agency, Ireland
Institution: NUI Galway
Publisher(s): Hindawi Publishing Corporation
File Format(s): application/pdf
First Indexed: 2019-09-20 06:39:12 Last Updated: 2019-09-20 06:39:12