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In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue
Chisholm, K. I.; Ida, K. K.; Davies, A. L.; Papkovsky, Dmitri B.; Singer, M.; Dyson, A.; Tachtsidis, I.; Duchen, M. R.; Smith, K. J.
Live imaging of mitochondrial function is crucial to understand the important role played by these organelles in a wide range of diseases. The mitochondrial redox potential is a particularly informative measure of mitochondrial function, and can be monitored using the endogenous green fluorescence of oxidized mitochondrial flavoproteins. Here, we have observed flavoprotein fluorescence in the exposed murine cerebral cortex in vivo using confocal imaging; the mitochondrial origin of the signal was confirmed using agents known to manipulate mitochondrial redox potential. The effects of cerebral oxygenation on flavoprotein fluorescence were determined by manipulating the inspired oxygen concentration. We report that flavoprotein fluorescence is sensitive to reductions in cortical oxygenation, such that reductions in inspired oxygen resulted in loss of flavoprotein fluorescence with the exception of a preserved ‘halo’ of signal in periarterial regions. The findings are consistent with reports that arteries play an important role in supplying oxygen directly to tissue in the cerebral cortex, maintaining mitochondrial function.
Keyword(s): Brain cortex; Mitochondria; Capillaries; Tension; Pressure; Venules; Surface; Disease; Oxygen; Brain; Vasculature; Confocal microscope
Publication Date:
2016
Type: Conference item
Peer-Reviewed: Yes
Language(s): English
Institution: University College Cork
Citation(s): Chisholm, K. I., Ida, K. K., Davies, A. L., Papkovsky, D. B., Singer, M., Dyson, A., Tachtsidis, I., Duchen, M. R. and Smith, K. J. (2016) 'In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue', in Elwell, C. E., Leung, T. S. and Harrison, D. K. (eds) Oxygen Transport to Tissue XXXVII. Advances in Experimental Medicine and Biology, vol 876. Springer, New York, NY, pp. 233-239. doi: 10.1007/978-1-4939-3023-4_29
Publisher(s): Springer Nature Switzerland AG
File Format(s): application/pdf
Related Link(s): http://www.isott.org/index.php/news-reader/items/isott-2014-london-uk.html,
https://doi.org/10.1007/978-1-4939-3023-4_66
First Indexed: 2019-10-26 07:22:48 Last Updated: 2019-10-26 07:22:48