Institutions | About Us | Help | Gaeilge
rian logo


Mark
Go Back
Behaviourally induced changes in ectracellular levels of brain glutamate monitored at 1 s resolution with an implanted biosensor
Lowry, John P.; Ryan, Michael R.; O'Neill, Robert D.
A biosensor for L-glutamic acid (Glu), constructed by immobilisation of L-glutamate oxidase onto lipid-coated 60 ┬Ám radius Pt wire with poly(o-phenylenediamine), was used amperometrically in attempts to detect glutamate in brain extracellular fluid (ECF) in the awake, freely moving rat at 1 s intervals. An implanted carbon paste electrode served to monitor simultaneous changes in brain ascorbate (AA). There were spontaneous, parallel changes in both signals, depending on the behavioural state of the animal, giving rise to a significant positive correlation coefficient between the two variables. The application of a mild stressor (10 s tail pinch) caused an immediate increase in both signals. The effects of inhibiting energy metabolism, together with an existing hypothesis linking Glu and AA in the ECF, were used to conclude that AA does not interfere electrochemically with the biosensor in vivo, that there are parallel changes in extracellular Glu and AA concentrations under normal physiological conditions, and that sub-micromolar physiologically related changes in brain Glu could be detected in the ECF several days after implantation of the electrodes.
Keyword(s): Behaviourally induced changes; ectracellular levels; brain glutamate; 1 s resolution; implanted biosensor; L-glutamic acid (Glu)
Publication Date:
1998
Type: Journal article
Peer-Reviewed: Yes
Institution: Maynooth University
Citation(s): Lowry, John P. and Ryan, Michael R. and O'Neill, Robert D. (1998) Behaviourally induced changes in ectracellular levels of brain glutamate monitored at 1 s resolution with an implanted biosensor. Analytical Communications, 35. pp. 87-89. ISSN 1359-7337
Publisher(s): Royal Society of Chemistry
File Format(s): other
Related Link(s): https://mural.maynoothuniversity.ie/8055/1/JL-Behaviourally-1998.pdf
First Indexed: 2020-04-02 06:32:53 Last Updated: 2021-07-31 08:00:59