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Continuous monitoring of extracellular glucose concentrations in the striatum of freely moving rats with an implanted glucose biosensor
Lowry, John P.; O'Neill, Robert D.; Boutelle, M.G.; Fillenz, Marianne
We have used a glucose oxidase-based sensor implanted in the striatum of freely moving rats to determine the concentration of extracellular glucose in two distinct ways. With a modification of the zero net flux method, in which different concentrations of glucose are infused through a dialysis probe glued to the biosensor, we calculated the concentration at which there was no change in glucose current by regression analysis; this gave a concentration of 0.351 ± 0.016 mM. Calculating the concentration from the basal current and the in vitro calibration of the biosensor was not significantly different from this. The basal extracellular glucose concentration determined by either method remained constant over a period of several days. Infusion of 50 µM veratridine through the adjacent dialysis probe caused a steep decrease in glucose current as soon as the drug reached the brain in contrast to the delayed fall (7.5 min) seen with microdialysis in previous experiments from this laboratory. These results demonstrate that this biosensor provides a direct, real-time measure of the extracellular concentration of glucose.
Keyword(s): Glucose biosensor; Brain glucose concentration; Veratridine; Rat striatum
Publication Date:
1998
Type: Journal article
Peer-Reviewed: Yes
Institution: Maynooth University
Citation(s): Lowry, John P. and O'Neill, Robert D. and Boutelle, M.G. and Fillenz, Marianne (1998) Continuous monitoring of extracellular glucose concentrations in the striatum of freely moving rats with an implanted glucose biosensor. Journal of Neurochemistry, 70 (1). pp. 391-396. ISSN 0022-3042
Publisher(s): Blackwell Publishing Ltd.
File Format(s): other
Related Link(s): https://mural.maynoothuniversity.ie/8057/1/JL-Continuous_1998.pdf
First Indexed: 2020-04-02 06:32:55 Last Updated: 2021-07-31 08:00:58