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Characterisation of carbon paste electrodes for real-time amperometric monitoring of brain tissue oxygen.
Bolger, Fiachra B.; McHugh, Stephen B.; Bennett, Rachel; Li, J.; Ishiwari, K.; Francois, Jennifer; Conway, Michael W.; Gilmour, Gary; Bannerman, David M.; Fillenz, Marianne; Tricklebank, Mark; Lowry, John P.
Tissue O₂ can be monitored using a variety of electrochemical techniques and electrodes. In vitro and in vivo characterisation studies for O₂ reduction at carbon paste electrodes (CPEs) using constant potential amperometry (CPA) are presented. Cyclic voltammetry indicated that an applied potential of -650 mV is required for O₂ reduction at CPEs. High sensitivity (-1.49 ± 0.01 nA/μM), low detection limit (ca. 0.1 μM) and good linear response characteristics (R² > 0.99) were observed in calibration experiments performed at this potential. There was also no effect of pH, temperature, and ion changes, and no dependence upon flow/fluid convection (stirring). Several compounds (e.g. dopamine and its metabolites) present in brain extracellular fluid were tested at physiological concentrations and shown not to interfere with the CPA O₂ signal. In vivo experiments confirmed a sub-second response time observed in vitro and demonstrated long-term stability extending over twelve weeks, with minimal O₂ consumption (ca. 1 nmol/h). These results indicate that CPEs operating amperometrically at a constant potential of -650 mV (vs. SCE) can be used reliably to continuously monitor brain extracellular tissue O₂.
Keyword(s): Carbon paste electrodes; Constant potential amperometry; Brain tissue oxygen; In vivo electrochemistry; Sensors
Publication Date:
Type: Journal article
Peer-Reviewed: Yes
Institution: Maynooth University
Citation(s): Bolger, Fiachra B. and McHugh, Stephen B. and Bennett, Rachel and Li, J. and Ishiwari, K. and Francois, Jennifer and Conway, Michael W. and Gilmour, Gary and Bannerman, David M. and Fillenz, Marianne and Tricklebank, Mark and Lowry, John P. (2011) Characterisation of carbon paste electrodes for real-time amperometric monitoring of brain tissue oxygen. Journal of Neuroscience Methods, 195 (2). pp. 135-142. ISSN 0165-0270
Publisher(s): Elsevier
File Format(s): other
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First Indexed: 2020-04-02 06:33:11 Last Updated: 2021-07-31 08:01:30