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Oxidative Stress Induced by Pt(IV) Pro-drugs Based on the Cisplatin Scaffold and Indole Carboxylic Acids in Axial Position
Tolan, Dina; Gandin, Valentina; Morrison, Liam; El-Nahas, Ahmed; Marzano, Cristina; Montagner, Diego; Erxleben, Andrea
The use of Pt(IV) complexes as pro-drugs that are activated by intracellular reduction is a widely investigated approach to overcome the limitations of Pt(II) anticancer agents. A series of ten mono- and bis-carboxylated Pt(IV) complexes with axial indole-3-acetic acid (IAA) and indole-3-propionic acid (IPA) ligands were synthesized and characterized by elemental analysis, ESI-MS, FT-IR, 1H and 195Pt NMR spectroscopy. Cellular uptake, DNA platination and cytotoxicity against a panel of human tumor cell lines were evaluated. All the complexes are able to overcome cisplatin-resistance and the most potent complex, cis,cis,trans-[Pt(NH3)2Cl2(IPA)(OH)] was on average three times more active than cisplatin. Mechanistic studies revealed that the trend in cytotoxicity of the Pt(IV) complexes is primarily consistent with their ability to accumulate into cancer cells and to increase intracellular basal reactive oxygen species levels, which in turn results in the loss of mitochondrial membrane potential and apoptosis induction. The role of the indole acid ligand as a redox modulator is discussed.
Keyword(s): Oxidative Stress; Pt(IV) complexes; Pro-drugs; Cisplatin Scaffold; Indole Carboxylic Acids
Publication Date:
2016
Type: Journal article
Peer-Reviewed: Yes
Institution: Maynooth University
Citation(s): Tolan, Dina and Gandin, Valentina and Morrison, Liam and El-Nahas, Ahmed and Marzano, Cristina and Montagner, Diego and Erxleben, Andrea (2016) Oxidative Stress Induced by Pt(IV) Pro-drugs Based on the Cisplatin Scaffold and Indole Carboxylic Acids in Axial Position. Scientific Reports, 6 (29367). ISSN 2045-2322
Publisher(s): Nature Publishing Group
File Format(s): other
Related Link(s): http://mural.maynoothuniversity.ie/7815/1/DM-Oxidative-2016.pdf
First Indexed: 2020-04-02 06:35:01 Last Updated: 2020-04-02 06:35:01