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Reversal of a Single Base-Pair Step Controls Guanine Photo-Oxidation by an Intercalating Ruthenium(II) Dipyridophenazine Complex
Keane, Páraic M.; Poynton, Fergus E.; Hall, James P.; Quinn, Susan J.
Small changes in DNA sequence can often have major biological effects. Here the rates and yields of guanine photo-oxidation by Λ-[Ru(TAP)2(dppz)]2+ have been compared in 5′-{CCGGATCCGG}2 and 5′-{CCGGTACCGG}2 using pico/nanosecond transient visible and time-resolved IR (TRIR) spectroscopy. The inefficiency of electron transfer in the TA sequence is consistent with the 5′-TA-3′ versus 5′-AT-3′ binding preference predicted by X-ray crystallography. The TRIR spectra also reveal the differences in binding sites in the two oligonucleotides. Irish Research Council Science Foundation Ireland Royal Irish Academy/Royal Society International Exchange Scheme UK Biotechnology and Biological Sciences Research Council College of Science, UCD UK Science and Technology Facilities Council
Keyword(s): Ruthenium; Guanine; Phenazines; DNA; Intercalating agents; Crystallography, X-ray; Base sequence; Base pairing; Oxidation-reduction; Light; Models, molecular; Coordination complexes
Publication Date:
Type: Journal article
Peer-Reviewed: Unknown
Language(s): English
Institution: University College Dublin
Publisher(s): Wiley
File Format(s): other
First Indexed: 2020-10-01 06:22:19 Last Updated: 2020-10-01 06:22:19