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Reversal of a Single Base-Pair Step Controls Guanine Photo-Oxidation by an Intercalating Ruthenium(II) Dipyridophenazine Complex |
Keane, Páraic M.; Poynton, Fergus E.; Hall, James P.; Quinn, Susan J.
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Small changes in DNA sequence can often have major biological effects. Here the rates and yields of guanine photo-oxidation by Λ-[Ru(TAP)2(dppz)]2+ have been compared in 5′-{CCGGATCCGG}2 and 5′-{CCGGTACCGG}2 using pico/nanosecond transient visible and time-resolved IR (TRIR) spectroscopy. The inefficiency of electron transfer in the TA sequence is consistent with the 5′-TA-3′ versus 5′-AT-3′ binding preference predicted by X-ray crystallography. The TRIR spectra also reveal the differences in binding sites in the two oligonucleotides. Irish Research Council Science Foundation Ireland Royal Irish Academy/Royal Society International Exchange Scheme UK Biotechnology and Biological Sciences Research Council College of Science, UCD UK Science and Technology Facilities Council
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Keyword(s):
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Ruthenium; Guanine; Phenazines; DNA; Intercalating agents; Crystallography, X-ray; Base sequence; Base pairing; Oxidation-reduction; Light; Models, molecular; Coordination complexes |
Publication Date:
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2020 |
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Type:
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Journal article |
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Peer-Reviewed:
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Unknown |
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Language(s):
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English |
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Institution:
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University College Dublin |
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Publisher(s):
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Wiley |
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File Format(s):
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other |
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First Indexed:
2020-10-01 06:22:19 Last Updated:
2020-10-01 06:22:19 |
