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Atrazine analysis using an amperometric immunosensor based on single-chain antibody fragments and regeneration-free multi-calibrant measurement.
Grennan, Kathleen; Strachan, Gillian; Porter, Andrew J.; Killard, Anthony J.; Smyth, Malcolm R.
This work describes the development of an electrochemical immunosensor for the analysis of atrazine using recombinant single-chain antibody (scAb) fragments. The sensors are based on carbon paste screen-printed electrodes incorporating the conducting polymer polyaniline (PANI)/poly(vinylsulphonic acid) (PVSA), which enables direct mediatorless coupling to take place between the redox centres of antigen-labelled horseradish peroxidase (HRP) and the electrode surface. Competitive immunoassays can be performed in real-time using this separation-free system. Analytical measurements based on the pseudo-linear relationship between the slope of a real-time amperometric signal and the concentration of analyte, yield a novel immunosensor set-up capable of regenerationless amperometric analysis. Multiple, sequential measurements of standards and samples can be performed on a single scAb-modified surface in a matter of minutes. No separation of bound and unbound species was necessary prior to detection. The system is capable of measuring atrazine to a detection limit of 0.1 ppb (0.1 μg l[-1]). This system offers the potential for rapid, cost-effective immunosensing for the analysis of samples of environmental, medical and pharmaceutical significance.
Keyword(s): Electrochemistry; Chemistry; screen-printed electrode; amperometric; scab; multi-calibrant analysis; atrazine;
Publication Date:
2003
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Institution: Dublin City University
Citation(s): Grennan, Kathleen and Strachan, Gillian and Porter, Andrew J. and Killard, Anthony J. and Smyth, Malcolm R. (2003) Atrazine analysis using an amperometric immunosensor based on single-chain antibody fragments and regeneration-free multi-calibrant measurement. Analytica Chimica Acta, 500 (1-2). pp. 287-298. ISSN 0003-2670
Publisher(s): Elsevier
File Format(s): application/pdf
Related Link(s): http://doras.dcu.ie/24/1/ACA3.pdf
First Indexed: 2014-04-17 05:43:19 Last Updated: 2018-05-15 06:19:52