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Biochemical Markers of the Mechanical Quality of Engineered Hyaline Cartilage
peer-reviewed The aim of this study was to determine whether or not biochemical markers can be used as surrogate measures for the mechanical quality of tissue engineered cartilage. The biochemical composition of tissue engineered cartilage constructs were altered by varying either (i) the initial cell seeding density of the scaffold (seeding density protocol) or (ii) the length of time the engineered tissue was cultured (culture period protocol). The aggregate or Young?s moduli of the constructs were measured (by confined or unconfined compression respectively), and compared with the composition of the extracellular matrix by quantitative measurement of the glycosaminoglycan (GAG), hydroxyproline, collagen I and collagen II and collagen cross-links. The aggregate modulus correlated positively with both GAG and collagen II content, but not with collagen I content. Young?s modulus correlated positively with GAG, collagen II and collagen I content, and the ratio of mature to immature cross-links, but not with hydroxyproline content. These results suggested that hydroxyproline may be an unreliable indicator of mechanical quality of tissue engineered cartilage, and that a measure of collagen II and GAG content is required to predict the biomechanical quality of tissue engineered cartilage.
Keyword(s): Administrative Staff Authors
Publication Date:
Type: Journal article
Peer-Reviewed: Yes
Language(s): English
Institution: Trinity College Dublin
Citation(s): D.J. Kelly, A. Crawford, S.C. Dickinson, T.J. Sims, A.P. Hollander, P.J. Prendergast and P.V. Hatton `Biochemical markers of the mechanical quality of engineered hyaline cartilage? in Journal of Materials Science: Materials in Medicine, 18, (2), 2007, pp 273-281
Publisher(s): Springer
File Format(s): application/pdf
First Indexed: 2014-05-13 05:11:20 Last Updated: 2017-04-26 09:30:38