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Conjugation at the oligonucleotide level based on isoxazole phosphoramidites generated by click chemistry
Freeman, Colin; Ni Cheallaigh, Aisling; Heaney, Frances
The versatility of the isoxazole generating nitrile oxide–alkyne Huisgen cycloaddition for provision of chemically modified oligonucleotides has been extended; in a novel approach isoxazole conjugated oligodeoxyribonucleotides have been constructed by phosphoramidite chemistry of isoxazole derivatives previously generated by nitrile oxide–alkyne click chemistry. The conjugation involves manual solid phase synthesis at room temperature in aqueous ethanol and proceeds in high yield.
Keyword(s): Chemistry; Conjugation; oligonucleotide; isoxazole phosphoramidites; click chemistry
Publication Date:
2011
Type: Journal article
Peer-Reviewed: Yes
Institution: Maynooth University
Citation(s): Freeman, Colin and Ni Cheallaigh, Aisling and Heaney, Frances (2011) Conjugation at the oligonucleotide level based on isoxazole phosphoramidites generated by click chemistry. Tetrahedron, 67 (40). pp. 7860-7865. ISSN 0040-4020
Publisher(s): Elsevier
File Format(s): application/pdf
Related Link(s): http://eprints.maynoothuniversity.ie/4292/1/FH_conjugation.pdf
First Indexed: 2014-09-20 05:02:07 Last Updated: 2014-09-20 05:02:07