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Current Search:
'Biochemistry' in all fields;
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Displaying Results 226 - 250 of 301 on page 10 of 13
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Investigation of response and resistance to dasatinib in melanoma cell lines
(2010)
Eustace, Alexander J
Investigation of response and resistance to dasatinib in melanoma cell lines
(2010)
Eustace, Alexander J
http://doras.dcu.ie/15432/
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Configurable and Up-Scalable Microfluidic Life Science Platform for Cell Based Assays by Gravity Driven Sequential Perfusion and Diffusion
(2010)
Dimov, Ivan Krastev
Configurable and Up-Scalable Microfluidic Life Science Platform for Cell Based Assays by Gravity Driven Sequential Perfusion and Diffusion
(2010)
Dimov, Ivan Krastev
Abstract:
Microfluidics has the potential to significantly change the way modern biology is performed, but for this potential to be realized several on-chip integration and operation challenges have to be addressed. Critical issues are addressed in this work by first demonstrating an integrated microfluidic tmRNA purification and real time nucleic acid sequence based amplification (NASBA) device. The device is manufactured using soft lithography and a unique silica bead immobilization method for the nucleic acid micro purification column. The integrated device produced a pathogen-specific response in < 3 min from the chip-purified RNA. Further enhancements in the device design and operation that allow the on-chip integration of mammalian cell handling and culturing produced a novel integrated NASBA array. This system demonstrated for the first time that it is possible to combine on a single micro-device cell culture and real time NASBA. In order to expand the cell based assay capabilities ...
http://doras.dcu.ie/15334/
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Development and application of novel analytical methods for molecularly targeted cancer therapeutics
(2011)
Roche, Sandra
Development and application of novel analytical methods for molecularly targeted cancer therapeutics
(2011)
Roche, Sandra
Abstract:
A detailed understanding of correlations of drug levels with drug action is an important aspect of the pre-clinical, clinical development and routine use of anti-cancer drugs. In furtherance of this important aim, the research in this thesis sought to develop and apply novel, highly sensitive, analytical methods to examine and understand correlations of drug levels with drug resistance mechanisms, therapeutic efficacy and side effects. LC-MS/MS is a state of the art analytical technique used to characterise and quantify both bio-molecules and pharmaceutical agents. Based on LC-MS/MS techniques, novel sensitive analytical methods were developed for the quantification of 1) tyrosine kinase inhibitor anti-cancer drugs to examine the interaction of these agents with drug resistance mechanisms, and 2) the multiple myeloma drug thalidomide as a pilot study to identify potential correlations between serum drug levels and toxicity/efficacy. Acquired resistance to chemotherapeutics throu...
http://doras.dcu.ie/16073/
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Synthesis of novel mast cell-stabilising and anti-allergic 1,2,3,4-tetrahydro-1-naphthalenols and related compounds
(2011)
WALSH, JOHN JARLATH
Synthesis of novel mast cell-stabilising and anti-allergic 1,2,3,4-tetrahydro-1-naphthalenols and related compounds
(2011)
WALSH, JOHN JARLATH
Abstract:
In a continuation of our studies into indan and tetralin systems as novel modulators of allergic and inflammatory phenomena, we have extended our work to include dimers linked via C-C bonds. Of these compounds, 2-Benzyl-2-[2-phenyl-1-ethenyl]-1,2,3,4-tetrahydro-1-naphthalenol 12 exhibited most promising activity both in vitro and in vivo.
http://hdl.handle.net/2262/54916
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Purification and Properties of Amycolatopsis Mediterranei DSM 43304 Lipase and Its Potential in Flavour Ester Synthesis
(2010)
Dheeman, Dharmendra Singh; Henehan, Gary T.M.; Frias, Jesus Maria
Purification and Properties of Amycolatopsis Mediterranei DSM 43304 Lipase and Its Potential in Flavour Ester Synthesis
(2010)
Dheeman, Dharmendra Singh; Henehan, Gary T.M.; Frias, Jesus Maria
Abstract:
<p>An extracellular thermostable lipase from <em>Amycolatopsis mediterranei</em> DSM 43304 has been purified to homogeneity using ammonium sulphate precipitation followed by anion exchange chromatography and hydrophobic interaction chromatography. This protocol resulted in 398 fold purification with 36% final recovery. The purified <em>A. mediterranei</em> DSM 43304 lipase (AML) has an apparent molecular mass of 33 kDa. The N-terminal sequence, AANPYERGPDPTTASIEATR, showed highest similarity to a lipase from <em>Streptomyces exfoliatus</em>. The values of and for <em>p</em>-nitrophenyl palmitate (<em>p</em>-NPP) under optimal temperature (60°C) and pH (8.0) conditions were 0.10 ± 0.01 mM and 2.53 ± 0.06 mmol/min<sup> </sup>mg, respectively. The purified AML displayed significant activity towards a range of short and long chain triglycerides. It was most active on triolein and a wide range of <em>p...
http://arrow.dit.ie/schfsehart/64
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The Preservation of in vivo Phosphorylated and Activated Uncoupling Protein 3 (UCP3) in Isolated Skeletal Muscle Mitochondria following Administration of 3,4-Methylenedioxymethamphetamine (MDMA aka Ecstasy) to Rats/Mice
(2012)
MEEGAN, MARY JANE; PORTER, RICHARD KEVIN; MCNAMARA, YVONNE; KELLY, ORLAGH
The Preservation of in vivo Phosphorylated and Activated Uncoupling Protein 3 (UCP3) in Isolated Skeletal Muscle Mitochondria following Administration of 3,4-Methylenedioxymethamphetamine (MDMA aka Ecstasy) to Rats/Mice
(2012)
MEEGAN, MARY JANE; PORTER, RICHARD KEVIN; MCNAMARA, YVONNE; KELLY, ORLAGH
Abstract:
Previous researchers have demonstrated that 3,4-methylenedioxymethamphetamine (MDMA) induced hyperthermia, in skeletal muscle of animals, is uncoupling protein 3 (UCP3) dependent. In light of our investigations that in vivo phosphorylation of UCP1 is augmented under conditions of cold-acclimation, we set out to investigate whether (a) UCP3 was phosphorylated in vivo and (b) whether in vivo phosphorylation of UCP3 resulted in increased proton leak following MDMA administration to animals. Our data demonstrate that MDMA treatment (but not PBS treatment) of animals results in both in vivo serine and tyrosine phosphorylation of UCP3 in skeletal muscle mitochondria, isolated in the presence of phosphatase inhibitors to preserve in vivo phosphorylation. In addition, proton leak is only increased in skeletal muscle mitochondria isolated from MDMA treated animals (in the presence of phosphatase inhibitors) and the increased proton leak is due to phosphorylated UCP3. UCP3 abundance in skelet...
http://hdl.handle.net/2262/55878
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Identification of Plasmepsin Inhibitors as Selective Antimalarial Agents using Ligand Based Drug Design
(2011)
DEMPSEY, ENDA; LLOYD, DAVID G; CARTA, GIORGIO; FAYNE, DARREN; BELL, ANGUS
Identification of Plasmepsin Inhibitors as Selective Antimalarial Agents using Ligand Based Drug Design
(2011)
DEMPSEY, ENDA; LLOYD, DAVID G; CARTA, GIORGIO; FAYNE, DARREN; BELL, ANGUS
Abstract:
We describe the application of Ligand Based Virtual Screening technologies towards the discovery of novel Plasmepsin (PM) inhibitors, a family of malarial parasitic aspartyl proteases. Pharmacophore queries were used to screen vendor libraries in search of active and selective compounds. The virtual hits were biologically assessed for activity and selectivity using whole cell Plasmodium falciparum parasites and on target in PM II, PM IV and the closely related human homologue, Cathepsin D assays. Here we report the virtual screening highlights, structures of the hits and their demonstrated biological activity.
http://hdl.handle.net/2262/55880
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Structural insights into dissimilatory sulfite reductases: structure of desulforubidin from Desulfomicrobium norvegicum
(2011)
KHAN, AMIR RAFIQ; FRANKLIN, EDWARD
Structural insights into dissimilatory sulfite reductases: structure of desulforubidin from Desulfomicrobium norvegicum
(2011)
KHAN, AMIR RAFIQ; FRANKLIN, EDWARD
Abstract:
Dissimilatory sulfite reductases (dSiRs) are crucial enzymes in bacterial sulfur-based energy metabolism, which are likely to have been present in some of the earliest life forms on Earth. Several classes of dSiRs have been proposed on the basis of different biochemical and spectroscopic properties, but it is not clear whether this corresponds to actual physiological or structural differences. Here, we describe the first structure of a dSiR from the desulforubidin class isolated from Desulfomicrobium norvegicum. The desulforubidin (Drub) structure is assembled as α2β2γ2, in which two DsrC proteins are bound to the core [DsrA]2[DsrB]2 unit, as reported for the desulfoviridin (Dvir) structure from Desulfovibrio vulgaris. Unlike Dvir, four sirohemes and eight [4Fe–4S] clusters are present in Drub. However, the structure indicates that only two of the Drub coupled siroheme-[4Fe–4S] cofactors are catalytically active. Mass spectrometry studies of purified Drub and Dvir show that both pro...
http://hdl.handle.net/2262/55973
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Rapid and efficient cancer cell killing mediated by high-affinity death receptor homotrimerizing TRAIL variants
(2010)
Natoni, Alessandro; Szegezdi, Eva; Samali, Afshin
Rapid and efficient cancer cell killing mediated by high-affinity death receptor homotrimerizing TRAIL variants
(2010)
Natoni, Alessandro; Szegezdi, Eva; Samali, Afshin
Abstract:
The tumour necrosis factor family member TNF-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in a variety of cancer cells through the activation of death receptors 4 (DR4) and 5 (DR5) and is considered a promising anticancer therapeutic agent. As apoptosis seems to occur primarily via only one of the two death receptors in many cancer cells, the introduction of DR selectivity is thought to create more potent TRAIL agonists with superior therapeutic properties. By use of a computer-aided structure-based design followed by rational combination of mutations, we obtained variants that signal exclusively via DR4. Besides an enhanced selectivity, these TRAIL-DR4 agonists show superior affinity to DR4, and a high apoptosis-inducing activity against several TRAIL-sensitive and -resistant cancer cell lines in vitro. Intriguingly, combined treatment of the DR4-selective variant and a DR5-selective TRAIL variant in cancer cell lines signalling by both death receptors le...
http://hdl.handle.net/10379/1930
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Crystallization of an engineered RUN domain of Rab6-interacting protein 1/DENND5
(2011)
KHAN, AMIR RAFIQ; FERNANDES, HUMBERTO; FRANKLIN, EDWARD
Crystallization of an engineered RUN domain of Rab6-interacting protein 1/DENND5
(2011)
KHAN, AMIR RAFIQ; FERNANDES, HUMBERTO; FRANKLIN, EDWARD
Abstract:
Effectors of the Rab small GTPases are large multi-domain proteins which have proved difficult to express in soluble form in Escherichia coli. Generally, effectors are recruited to a distinct subcellular compartment by active (GTP-bound) Rabs, which are linked to membranes by one or two prenylated Cys residues at their C-termini. Following recruitment via their Rab-binding domain (RBD), effectors carry out various aspects of vesicle formation, transport, tethering and fusion through their other domains. Previously, successful purification of the RUN-PLAT tandem domains (residues 683-1061) of the 1263-residue Rab6-interacting protein 1 (R6IP1) required co-expression with Rab6, as attempts to solubly express the effector alone were unsuccessful. R6IP1 is also known as DENN domain-containing protein 5 (DENND5) and is expressed as two isoforms, R6IP1A/B (DENND5A/B), which differ by 24 amino acids at the N-terminus. Here, a deletion in R6IP1 was engineered to enable soluble expression an...
http://hdl.handle.net/2262/56025
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Tunable bromomagnesium thiolate Tishchenko reaction catalysts: efficient intermolecular aldehyde-trifluoromethylketone coupling
(2010)
CONNON, STEPHEN
Tunable bromomagnesium thiolate Tishchenko reaction catalysts: efficient intermolecular aldehyde-trifluoromethylketone coupling
(2010)
CONNON, STEPHEN
Abstract:
A new thiolate-catalyzed Tishchenko reaction inspired by biochemical processes features exceptionally good control over the catalyst's steric/electronic properties. The isolation of reaction intermediates provided valuable insights into the reaction mechanism, which in turn has allowed the development of the first cross-Tishchenko coupling reactions between aldehydes and activated ketones
http://hdl.handle.net/2262/56215
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Pneumolysin activates the NLRP3 inflammasome and promotes proinflammatory cytokines independently of TLR4.
(2010)
MORAN, BARRY; LAVELLE, EDWARD; MC LOUGHLIN, RACHEL MARY; MC NEELA, EDEL
Pneumolysin activates the NLRP3 inflammasome and promotes proinflammatory cytokines independently of TLR4.
(2010)
MORAN, BARRY; LAVELLE, EDWARD; MC LOUGHLIN, RACHEL MARY; MC NEELA, EDEL
Abstract:
Pneumolysin (PLY) is a key Streptococcus pneumoniae virulence factor and potential candidate for inclusion in pneumococcal subunit vaccines. Dendritic cells (DC) play a key role in the initiation and instruction of adaptive immunity, but the effects of PLY on DC have not been widely investigated. Endotoxin-free PLY enhanced costimulatory molecule expression on DC but did not induce cytokine secretion. These effects have functional significance as adoptive transfer of DC exposed to PLY and antigen resulted in stronger antigen-specific T cell proliferation than transfer of DC exposed to antigen alone. PLY synergized with TLR agonists to enhance secretion of the proinflammatory cytokines IL-12, IL-23, IL-6, IL-1β, IL-1α and TNF-α by DC and enhanced cytokines including IL-17A and IFN-γ by splenocytes. PLY-induced DC maturation and cytokine secretion by DC and splenocytes was TLR4-independent. Both IL-17A and IFN-γ are required for protective immunity to pneumococcal infection and intran...
http://hdl.handle.net/2262/56345
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Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis.
(2001)
MARTIN, SEAMUS JOSEPH
Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis.
(2001)
MARTIN, SEAMUS JOSEPH
Abstract:
Members of the caspase family of cysteine proteases play central roles in coordinating the stereotypical events that occur during apoptosis. Because the major executioner caspases, caspase-3 and caspase-7, exhibit almost indistinguishable activity toward certain synthetic peptide substrates, this has led to the widespread view that these proteases occupy functionally redundant roles within the cell death machinery. However, the distinct phenotypes of mice deficient in either of these caspases, as well as mice deficient in both, is at odds with this view. These distinct phenotypes could be related to differences in the relative expression levels of caspase-3 and caspase-7 in vivo, or due to more fundamental differences between these proteases in terms of their ability to cleave natural substrates. Here we show that caspase-3 and caspase-7 exhibit differential activity toward multiple substrate proteins, including Bid, XIAP, gelsolin, caspase-6, and cochaperone p23. Caspase-3 was foun...
http://hdl.handle.net/2262/56334
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Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
(2009)
MARTIN, SEAMUS JOSEPH
Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
(2009)
MARTIN, SEAMUS JOSEPH
Abstract:
Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/βNAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, βNAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also res...
http://hdl.handle.net/2262/56335
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Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes.
(2009)
MARTIN, SEAMUS JOSEPH
Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes.
(2009)
MARTIN, SEAMUS JOSEPH
Abstract:
Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their ...
http://hdl.handle.net/2262/56336
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Synthesis and characterization of biomimetic hydroxyapatite/sepiolite nanocomposites
(2011)
CHEN, BIQIONG; WAN, CHAOYING
Synthesis and characterization of biomimetic hydroxyapatite/sepiolite nanocomposites
(2011)
CHEN, BIQIONG; WAN, CHAOYING
Abstract:
Natural fibrous sepiolite with a high surface area, negative surface charge and porous structure is promising for hydroxyapatite (HAp) mineralization since the clay is naturally abundant and biocompatible. In this paper, the use of fibrous sepiolite as a template for growth of HAp nanocrystals was reported for the first time. Carbonated HAp nanorods with dimensions of 20–60 nm in length and 10–20 nm in diameter were successfully grown on the sepiolite surface with a preferred orientation to the c-axis. The critical nucleus radius of HAp in the presence of natural sepiolite was estimated as 0.296–0.312 nm. Strong acid-activation increased the specific surface area of the sepiolite by 205% and also transformed the sepiolite to silica fiber with an elastic modulus being 395% of the original value. The novel HAp/acid-activated sepiolite biocomposite has a specific surface area of 182 m2 g−1 and an elastic modulus of over 20 GPa, considerably higher than those of the HAp synthesized with...
http://hdl.handle.net/2262/56344
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Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system
(2002)
Daly, Stephen J.; Dillon, Paul P.; Manning, Bernadette M.; Dunne, Lynsey; Killard, Anth...
Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system
(2002)
Daly, Stephen J.; Dillon, Paul P.; Manning, Bernadette M.; Dunne, Lynsey; Killard, Anthony J.; O'Kennedy, Richard
Abstract:
The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB[SUB1], in this format, with the assays being sensitive and reproducible.
http://doras.dcu.ie/47/
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Chemical microscopy and nanoscopy of bio-materials and living cells, IRMMW-THz
(2010)
KOPF, ILONA
Chemical microscopy and nanoscopy of bio-materials and living cells, IRMMW-THz
(2010)
KOPF, ILONA
Abstract:
Raman microspectroscopy of human sperm cells and nearfield infrared microscopy of nanoscale biomaterials like nanografted DNA have been performed. A newly developed station for the ANKA-IR2 beamline combines several techniques to study the same sample at the same position.
http://hdl.handle.net/2262/56559
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Recommendations for terminology and databases for biochemical thermodynamics
(2011)
TIPTON, KEITH
Recommendations for terminology and databases for biochemical thermodynamics
(2011)
TIPTON, KEITH
Abstract:
Chemical equations are normally written in terms of specific ionic and elemental species and balance atoms of elements and electric charge. However, in a biochemical context it is usually better to write them with ionic reactants expressed as totals of species in equilibrium with each other. This implies that atoms of elements assumed to be at fixed concentrations, such as hydrogen at a specified pH, should not be balanced in a biochemical equation used for thermodynamic analysis. However, both kinds of equations are needed in biochemistry. The apparent equilibrium constant K′ for a biochemical reaction is written in terms of such sums of species and can be used to calculate standard transformed Gibbs energies of reaction ΔrG′o. This property for a biochemical reaction can be calculated from the standard transformed Gibbs energies of formation ΔfGi′° of reactants, which can be calculated from the standard Gibbs energies of formation of species ΔfGj° and measured apparent equilibrium...
http://hdl.handle.net/2262/56818
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Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap.
(2011)
WRIDE, MICHAEL; FARRAR, GWYNETH JANE; BAZOU, DESPINA; MANSERGH, FIONA
Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap.
(2011)
WRIDE, MICHAEL; FARRAR, GWYNETH JANE; BAZOU, DESPINA; MANSERGH, FIONA
Abstract:
In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08–0.85 MPa) and times (5–60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine.
http://hdl.handle.net/2262/56905
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Excited state behaviour of substituted dipyridophenazine Cr(III) complexes in the presence of nucleic acids.
(2010)
KELLY, JOHN MOFFAT; QUINN, SUSAN
Excited state behaviour of substituted dipyridophenazine Cr(III) complexes in the presence of nucleic acids.
(2010)
KELLY, JOHN MOFFAT; QUINN, SUSAN
Abstract:
The photophysics and photochemistry of [Cr(phen)2(dppz)]3+ and its 11,12-substituted derivatives [Cr(phen)2(X2dppz)]3+ {X = Me or F} have been studied in the presence of purine nucleotides or DNA using steady state and time-resolved absorption and luminescence spectroscopy . 5′-Adenosine monophosphate (5′-AMP) shows only a weak interaction with the excited states of each complex. By contrast they are efficiently quenched by 5′-guanosine monophosphate (5′-GMP), consistent with photo-induced electron transfer . Laser flash photolysis spectroscopy in the presence of 5′-GMP suggests that both forward and back electron-transfers are rapid. All complexes also display a strong affinity for DNA and evidence for both static and dynamic quenching mechanisms is provided.
http://hdl.handle.net/2262/57287
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Maternal Health Behaviours During Pregnancy and Associations with Infant Feeding Factors
(2010)
Tarrant, Roslyn C; Younger, Katherine M; Sheridan-Pereira, Margaret; Kearney, John M
Maternal Health Behaviours During Pregnancy and Associations with Infant Feeding Factors
(2010)
Tarrant, Roslyn C; Younger, Katherine M; Sheridan-Pereira, Margaret; Kearney, John M
Abstract:
<p><strong>Maternal health behaviours during pregnancy and associations with postnatal infant feeding practices</strong></p> <p><em>Objectives:</em> To examine modifiable maternal health behaviours during pregnancy, including folic acid use peri-conceptually, smoking and alcohol consumption patterns, and relate these factors to maternal postnatal infant feeding practices.</p> <p><em>Materials and Methods:</em> A prospective observational study involved the recruitment of 539 pregnant women from a Dublin maternity hospital. Following application of exclusion criteria, 450 mothers were followed up at 6 weeks and 6 months postpartum. Quantitative data on folic acid use, as well as mothers’ smoking and alcohol consumption patterns during pregnancy were collected from the antenatal questionnaire. Maternal postnatal infant feeding practices (breastfeeding/weaning data) were recorded from the 6 week and 6 month interviews.&l...
http://arrow.dit.ie/scschbioth/3
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Temperature Dependent Electrochemistry - a Versatile Tool for Investigations of Biology Related Topics
(2009)
SCHULZKE, CAROLA
Temperature Dependent Electrochemistry - a Versatile Tool for Investigations of Biology Related Topics
(2009)
SCHULZKE, CAROLA
Abstract:
Temperature dependent electrochemistry can be efficiently used to determine very different properties of the investigated system, such as thermodynamic parameters of redox processes (especially the entropy), the degeneration temperature of a protein or kinetic parameters, for instance activation energy. It can even be used in biotechnology for improved catalysis and detection of substances. This perspective describes a selection of different experiments that used temperature dependent electrochemistry in order to determine these different values or achieve an enhancement of biotechnological applications, respectively, and hence gives an overview of its versatile use in studies aimed at biological issues.
http://hdl.handle.net/2262/57490
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Organic Functionalisation and Characterisation of Single-walled Carbon Nanotubes
(2009)
GIORDANI, SILVIA
Organic Functionalisation and Characterisation of Single-walled Carbon Nanotubes
(2009)
GIORDANI, SILVIA
Abstract:
Since carbon nanotubes (CNTs) display unique structures and remarkable physical properties, a variety of applications have emerged in both materials and life sciences. In terms of applications, the functionalisation of nanotubes is extremely important, as it increases their solubility and processability, and combines the unique properties of single-walled carbon nanotubes (SWCNTs) with those of other classes of materials. A number of methods have been developed, which can be divided into two major approaches: (1) non-covalent supramolecular modifications, and (2) covalent functionalisation. In this tutorial review, we survey the covalent modification of SWCNTs with organic moieties, and illustrate the major analytical techniques routinely used to characterise the functionalised materials.
http://hdl.handle.net/2262/57488
Marked
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Modulating topologies and magnetic properties of coordination polymers using 2,2-bipyridine and 5-aminodiacetic isophthalic acid as ligands
(2009)
SCHMITT, WOLFGANG
Modulating topologies and magnetic properties of coordination polymers using 2,2-bipyridine and 5-aminodiacetic isophthalic acid as ligands
(2009)
SCHMITT, WOLFGANG
Abstract:
We describe a synthetic methodology to prepare 1D coordination polymers using 5-aminodiacetic isophthalic acid (H4adip) as chelating agent and 2,2′-bipyridine (bpy) as an auxiliary ligand. The function of the latter is to block two coordination sites on the metal(II) centres thus avoiding the formation of 3D networks and directing the assembly into low dimensional networks. The compounds [CoII2(adip)(bpy)(H2O)4]·3H2O (1), [NiII2(adip)(bpy)(H2O)4]·3H2O (2) and [CuII(H2adip)(bpy)] (3) were synthesised under hydrothermal reaction conditions with the motivation to investigate spin interactions in these 1D chain structures. We report the structures of the compounds and investigate and model their magnetic properties. The supramolecular structures of 1–3 are characterised by inter- and intra-chain π–π interactions and hydrogen bonding.
http://hdl.handle.net/2262/57489
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