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Although Duchenne muscular dystrophy is primarily classified as a neuromuscular disease, cardiac complications play an
important role in the course of this X-linked inherited disorder. The pathobiochemical steps causing a progressive decline in
the dystrophic heart are not well understood. We therefore carried out a fluorescence difference in-gel electrophoretic analysis
of 9-month-old dystrophin-deficient versus age-matched normal heart, using the established MDX mouse model of muscular
dystrophy-related cardiomyopathy. Out of 2,509 detectable protein spots, 79 2D-spots showed a drastic differential expression
pattern, with the concentration of 3 proteins being increased, including nucleoside diphosphate kinase and lamin-A/C, and
of 26 protein species being decreased, including ATP synthase, fatty acid binding-protein, isocitrate dehydrogenase, NADH
dehydrogenase, porin, peroxiredoxin, adenylate kinase, tropomyosin, actin, and myosin light chains. Hence, the lack of cardiac
dystrop...
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