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Previous studies in this laboratory have demonstrated that exposure to 5-Bromo-2- deoxyuridine (BrdU) induces differentiation in the lung epithelial cancer cell lines DLKP and A549. This induction o f differentiation was accompanied by increased protein expression of cytokeratins-8 and -18, a 2Pi integrin, cell-adhesion protein Ep- CAM, eukaryotic initiation factor eIF-4E and the transcription factors c-myc and YY1. Following exposure to BrdU, RT-PCR analysis identified large increases in the mRNA levels of MRP1, MRP3, BCRP, BAXa, MRIT, COX-2 and eIF-2a, together with smaller gene expression increases for MRP2, a-catenin and E-cadherin, in the DLKP cell line. Survivin gene and protein expression was decreased. Increased gene expression of MRP 1 and BCRP, as well as decreased MRP2, MRP4 and mdr-1 gene expression was observed in A549. DNA microarray analysis of BrdU-treated DLKP detected significantly increased gene expression of ETR103, p55CDC, Inil, NSEP, EB1, RPS19 and FNRB, and si...
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